[1]王晨,袁德晓,邵春林.电离辐射诱导的外泌体的生物学效应[J].国际放射医学核医学杂志,2017,41(2):121-125.[doi:10.3760/cma.j.issn.1673-4114.2017.02.008]
 Wang Chen,Yuan Dexiao,Shao Chunlin.Biological effects of radiation-induced exosomes[J].International Journal of Radiation Medicine and Nuclear Medicine,2017,41(2):121-125.[doi:10.3760/cma.j.issn.1673-4114.2017.02.008]
点击复制

电离辐射诱导的外泌体的生物学效应(/HTML)
分享到:

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
41
期数:
2017年第2期
页码:
121-125
栏目:
综述
出版日期:
2017-03-25

文章信息/Info

Title:
Biological effects of radiation-induced exosomes
作者:
王晨 袁德晓 邵春林
200032 上海, 复旦大学放射医学研究所放射生物研究部
Author(s):
Wang Chen Yuan Dexiao Shao Chunlin
Department of Radiation Biology, Institute of Radiation Medicine, Fudan University, Shanghai 200032, China
关键词:
辐射电离肿瘤外泌体生物学效应
Keywords:
Radiation ionizingNeoplasmsExosomesBiological effectiveness
DOI:
10.3760/cma.j.issn.1673-4114.2017.02.008
摘要:
外泌体是一种在细胞内形成并主动释放到胞外的囊泡体,内含有大量蛋白质、脂质和非编码RNA等生物活性分子,可参与细胞间的通讯来调节多种重要的生理病理过程。研究发现辐射诱导的外泌体能够辅助肿瘤与微环境的相互交流。笔者在该文中集中讨论了辐射诱导的外泌体在肿瘤放疗过程中的生物学效应,重点关注其在肿瘤血管新生中的作用,并就这些方面的研究进展进行综述。
Abstract:
Exosomes are small extracellular vesicles released from normal and tumor cells. These vesicles contain proteins, lipids, and noncoding RNAs, and can mediate intercellular communication among different cell types in the body, thus affecting physiological and pathological processes. Emerging evidence indicates that radiation-induced exosomes facilitates the interaction between a tumor and its microenvironment. This article reviewed the biological effects of radiation-induced exosomes on tumor radiotherapy and discussed the role of such exosomes in tumor angiogenesis.

参考文献/References:

[1] Colombo M, Raposo G, Théry C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles[J]. Annu Rev Cell Dev Biol, 2014, 30:255-289. DOI:10. 1146/annurev-cellbio-101512-122326.
[2] Whiteside TL. Tumor-derived exosomes and their role in cancer progression[J/OL]. Adv Clin Chem, 2016, 74:103-141[2016-12-15]. http://www.sciencedirect.com/science/article/pii/S0065242315300056. DOI:10. 1016/bs. acc. 2015. 12. 005.
[3] Yu DD, Wu Y, Shen HY, et al. Exosomes in development, metastasis and drug resistance of breast cancer[J]. Cancer Sci, 2015, 106(8):959-964. DOI:10. 1111/cas. 12715.
[4] Hanson PI, Cashikar A. Multivesicular body morphogenesis[J/OL]. Annu Rev Cell Dev Biol, 2012, 28:337-362[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/22831642. DOI:10. 1146/annurev-cellbio-092910-154152.
[5] Milane L, Singh A, Mattheolabakis G, et al. Exosome mediated communication within the tumor microenvironment[J/OL]. J Control Release, 2015, 219:278-294[2016-12-15]. https://www.ncbi.nlm.nih.gov/pubmed/26143224. DOI:10. 1016/j. jconrel. 2015. 06. 029.
[6] Stuffers S, Sem Wegner C, Stenmark H, et al. Multivesicular endosome biogenesis in the absence of ESCRTs[J]. Traffic, 2009, 10(7):925-937. DOI:10. 1111/j. 1600-0854. 2009. 00920. x.
[7] Raposo G, Stoorvogel W. Extracellular vesicles:exosomes, microvesicles, and friends[J]. J Cell Biol, 2013, 200(4):373-383. DOI:10. 1083/jcb. 201211138.
[8] Savina A, Vidal M, Colombo MI. The exosome pathway in K562 cells is regulated by Rab11[J]. J Cell Sci, 2002, 115(Pt 12):2505-2515.
[9] Hsu C, Morohashi Y, Yoshimura S, et al. Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C[J]. J Cell Biol, 2010, 189(2):223-232. DOI:10. 1083/jcb. 200911018.
[10] Ostrowski M, Carmo NB, Krumeich S, et al. Rab27a and Rab27b control different steps of the exosome secretion pathway[J]. Nat Cell Biol, 2010, 12(1):19-30; suppp 1-13. DOI:10. 1038/ncb2000.
[11] Zylbersztejn K, Galli T. Vesicular traffic in cell navigation[J]. FEBS J, 2011, 278(23):4497-4505. DOI:10. 1111/j. 1742-4658. 2011. 08168. x.
[12] Mulcahy LA, Pink RC, Carter DR. Routes and mechanisms of extracellular vesicle uptake[J/OL]. J Extracell Vesicles, 2014[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/25143819. DOI:10. 3402/jev. v3. 24641.
[13] De Jong OG, Van Balkom BW, Schiffelers RM, et al. Extracellular vesicles:potential roles in regenerative medicine[J/OL]. Front Immunol, 2014, 5:608[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/25520717. DOI:10. 3389/fimmu. 2014. 00608.
[14] Kooijmans SA, Vader P, Van Dommelen SM, et al. Exosome mimetics:a novel class of drug delivery systems[J/OL]. Int J Nanomedicine, 2012, 7:1525-1541[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/22619510. DOI:10. 2147/IJN. S29661.
[15] Pant S, Hilton H, Burczynski ME. The multifaceted exosome:biogenesis, role in normal and aberrant cellular function, and frontiers for pharmacological and biomarker opportunities[J]. Biochem Pharmacol, 2012, 83(11):1484-1494. DOI:10. 1016/j. bcp. 2011. 12. 037.
[16] Khan S, Jutzy JM, Aspe JR, et al. Survivin is released from cancer cells via exosomes[J]. Apoptosis, 2011, 16(1):1-12. DOI:10. 1007/s10495-010-0534-4.
[17] Azzam EI, de Toledo SM, Little JB. Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from alpha-particle irradiated to nonirradiated cells[J]. Proc Natl Acad Sci U S A, 2001, 98(2):473-478. DOI:10. 1073/pnas. 011417098.
[18] Shao C, Folkard M, Prise KM. Role of TGF-β1 and nitric oxide in the bystander response of irradiated glioma cells[J]. Oncogene, 2008, 27(4):434-440. DOI:10. 1038/sj. onc. 1210653.
[19] Wang H, Yu KN, Hou J, et al. Radiation-induced bystander effect:early process and rapid assessment[J]. Cancer Lett, 2015, 356(1):137-144. DOI:10. 1016/j. canlet. 2013. 09. 031.
[20] Tkach M, Théry C. Communication by extracellular vesicles:where we are and where we need to go[J]. Cell, 2016, 164(6):1226-1232. DOI:10. 1016/j. cell. 2016. 01. 043.
[21] Al-Mayah AH, Irons SL, Pink RC, et al. Possible role of exosomes containing RNA in mediating nontargeted effect of ionizing radiation[J]. Radiat Res, 2012, 177(5):539-545. DOI:10. 1667/RR2868. 1.
[22] Jelonek K, Widlak P, Pietrowska M. The influence of ionizing radiation on exosome composition, secretion and intercellular communication[J]. Protein Pept Lett, 2016, 23(7):656-663. DOI:10. 2174/0929866523666160427105138.
[23] Al-Mayah A, Bright S, Chapman K, et al. The non-targeted effects of radiation are perpetuated by exosomes[J/OL]. Mutat Res, 2015, 772:38-45[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/25772109. DOI:10. 1016/j. mrfmmm. 2014. 12. 007.
[24] Jella KK, Rani S, O’Driscoll L, et al. Exosomes are involved in mediating radiation induced bystander signaling in human keratinocyte cells[J]. Radiat Res, 2014, 181(2):138-145. DOI:10. 1667/RR13337. 1.
[25] Jelonek K, Wojakowska A, Marczak L, et al. Ionizing radiation affects protein composition of exosomes secreted in vitro from head and neck squamous cell carcinoma[J]. Acta Biochim Pol, 2015, 62(2):265-272. DOI:10. 18388/abp. 2015_970.
[26] Hazawa M, Tomiyama K, Saotome-Nakamura A, et al. Radiation increases the cellular uptake of exosomes through CD29/CD81 complex formation[J]. Biochem Biophys Res Commun, 2014, 446(4):1165-1171. DOI:10. 1016/j. bbrc. 2014. 03. 067.
[27] Hui L, Chen Y. Tumor microenvironment:Sanctuary of the devil[J]. Cancer Lett, 2015, 368(1):7-13. DOI:10. 1016/j. canlet. 2015. 07. 039.
[28] Krishna Priya S, Nagare RP, Sneha VS, et al. Tumour angiogenesis-Origin of blood vessels[J]. Int J Cancer, 2016, 139(4):729-735. DOI:10. 1002/ijc. 30067.
[29] Xu S, Ding N, Pei H, et al. MiR-21 is involved in radiation-induced bystander effects[J]. RNA Biol, 2014, 11(9):1161-1170. DOI:10. 4161/rna. 34380.
[30] Xu S, Wang J, Ding N, et al. Exosome-mediated microRNA transfer plays a role in radiation-induced bystander effect[J]. RNA Biol, 2015, 12(12):1355-1363. DOI:10.1080/15476286.2015. 1100795.
[31] Liu Y, Luo F, Wang B, et al. STAT3-regulated exosomal miR-21 promotes angiogenesis and is involved in neoplastic processes of transformed human bronchial epithelial cells[J]. Cancer Lett, 2016, 370(1):125-135. DOI:10. 1016/j. canlet. 2015. 10. 011.
[32] Kulshreshtha R, Ferracin M, Wojcik SE, et al. A microRNA signature of hypoxia[J]. Mol Cell Biol, 2007, 27(5):1859-1867. DOI:10. 1128/MCB. 01395-06.
[33] Liu LZ, Li C, Chen Q, et al. MiR-21 induced angiogenesis through AKT and ERK activation and HIF-1α expression[J/OL]. PLoS One, 2011, 6(4):e19139[2016-12-15]. http://www.ncbi.nlm.nih.gov/pubmed/21544242. DOI:10. 1371/journal. pone. 0019139.

相似文献/References:

[1]徐畅,方连英,孔阳阳,等.沉默BLAP75基因对电离辐射诱导DNA损伤的影响[J].国际放射医学核医学杂志,2016,40(4):244.[doi:10.3760/cma.j.issn.1673-4114.2016.04.001]
 Xu Chang,Fang Lianying,Kong Yangyang,et al.Effects of silencing BLAP75 on DNA damage induced by ionizing radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):244.[doi:10.3760/cma.j.issn.1673-4114.2016.04.001]
[2]董娟聪,刘红艳,党旭红,等.12c重离子与γ射线对人外周血淋巴细胞peng-ebv增殖、细胞周期及凋亡影响的比较[J].国际放射医学核医学杂志,2015,39(5):385.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
 dong juancong,liu hongyan,dang xuhong,et al.effects of 12c heavy ion and γ ray irradiation in cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):385.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
[3]李晴,王璐,徐畅,等.姜油树脂对辐射后间充质干细胞nrf2及其靶基因表达的影响[J].国际放射医学核医学杂志,2015,39(5):389.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 008]
 li Qing,wang lu,xu chang,et al.ginger oleoresin induced expression of nrf2 and its target genes in mesenchymal stem cells after radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):389.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 008]
[4]徐金苹,袁德晓,张江虹,等.辐射诱导的外泌体在肿瘤细胞侵袭转移中的作用[J].国际放射医学核医学杂志,2015,39(2):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]
 Xu Jinping,Yuan Dexiao,Zhang Jianghong,et al.The role of radiation-induced exosomes in tumor invasion and metastasis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]
[5]王芹,王敬敏,徐畅,等.沉默XRCC2基因表达联合电离辐射对结肠癌细胞增殖能力的影响[J].国际放射医学核医学杂志,2015,39(4):282.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
 Wang Qin,Wang Jingmin,Xu Chang,et al.The effect of silencing XRCC2 gene combined with ionizing radiation on growth of colorectal cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):282.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
[6]施常备,许建林,袁勇,等.染色体畸变率估算32P的辐射剂量[J].国际放射医学核医学杂志,2012,36(1):35.[doi:10.3760/cma.j.issn.1673-4114.2012.01.009]
 SHI Chang-bei,XU Jian-lin,YUAN Yong,et al.The quantitative radiation dose of 32P by chromosomal aberration[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):35.[doi:10.3760/cma.j.issn.1673-4114.2012.01.009]
[7]赵欣然,江波,傅宝华,等.《过量照射人员的医学检查与处理原则》解读[J].国际放射医学核医学杂志,2012,36(4):207.[doi:10.3760/cma.j.issn.1673-4114.2012.04.003]
 ZHAO Xin-ran,JIANG Bo,FU Bao-hua,et al.Explanation of Medical Examination and Management Principle for the Overexposed Individuals[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):207.[doi:10.3760/cma.j.issn.1673-4114.2012.04.003]
[8]刘玉龙,姜忠.《核事故场内医学应急响应程序》解读[J].国际放射医学核医学杂志,2012,36(4):218.[doi:10.3760/cma.j.issn.1673-4114.2012.04.006]
 LIU Yu-long,JIANG Zhong.Explanation of Procedure on Site Medical Emergency Response for Nuclear Accident[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):218.[doi:10.3760/cma.j.issn.1673-4114.2012.04.006]
[9]刘强,王彦,杜利清,等.《单细胞凝胶电泳用于受照人员剂量估算技术规范》解读[J].国际放射医学核医学杂志,2012,36(4):242.[doi:10.3760/cma.j.issn.1673-4114.2012.04.012]
 LIU Qiang,WANG Yan,DU Li-qing,et al.Explanation for the Specification of Dose Estimation for the Radiation Victims in the Early Stage Using Single Cell Gel Electrophoresis[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):242.[doi:10.3760/cma.j.issn.1673-4114.2012.04.012]
[10]刘强,王彦.ICRP118关于辐射诱导组织反应内容的部分修改建议[J].国际放射医学核医学杂志,2013,37(6):389.[doi:10.3760/cma.j.issn.1673-4114.2013.06.016]
 LIU Qiang,WANG Yan.Revision recommendations of tissue reaction induced by radiation in ICRP118[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(2):389.[doi:10.3760/cma.j.issn.1673-4114.2013.06.016]
[11]张宇睿,徐文清.电离辐射对线粒体损伤的研究进展[J].国际放射医学核医学杂志,2016,40(2):154.[doi:10.3760/cma.j.issn.1673-4114.2016.02.014]
 Zhang Yurui,Xu Wenqing.Damages of ionizing radiation on mitochondria[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):154.[doi:10.3760/cma.j.issn.1673-4114.2016.02.014]
[12]张俊伶,薛晓蕾,李源,等.富氢水对电离辐射引起胸腺细胞损伤的影响[J].国际放射医学核医学杂志,2015,39(5):358.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 001]
 zhang junling,xue xiaolei,li yuan,et al.effects of hydrogen-rich water on radiation-induced thymus injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):358.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 001]
[13]付岳,王彦,杜利清,等.sirt1基因沉默在辐射诱导的nlrp3和il-1β表达中的作用研究[J].国际放射医学核医学杂志,2015,39(5):367.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
 fu yue*,wang yan,du liqing,et al.effects of nlrp3 and il-1β on radiation-induced expression through sirt1 gene silencing[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):367.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
[14]刘建功,党旭红,刘红艳,等.核工业某厂放射工作人员与非放射工作人员的mdm2基因表达调查与分析[J].国际放射医学核医学杂志,2015,39(5):416.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 015]
 liu jiangong,dang xuhong,liu hongyan,et al.investigation and analysis of mdm2 expression level in radiation-exposed workers and non-radiation-exposed workers[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):416.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 015]
[15]王璐,李晴,徐畅,等.sirna干扰sirt1对辐射后il-6表达的影响[J].国际放射医学核医学杂志,2015,39(5):423.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
 wang lu,li Qing,xu chang,et al.effect of sirt1 sirna interference on the expression of il-6 after radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):423.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
[16]张倩如,李海涛,田红旗.小分子辐射防护药物的研究进展[J].国际放射医学核医学杂志,2016,40(5):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
 Zhang Qianru,Li Haitao,Tian Hongqi.Small molecule compounds against radiation:research advances[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
[17]肖林林,金问森,张江虹,等.电离辐射诱发动脉粥样硬化的研究进展[J].国际放射医学核医学杂志,2015,39(6):493.[doi:10.3760/cma.j.issn.1673-4114.2015.06.012]
 Xiao Linlin,Jin Wensen,Zhang Jianghong,et al.Research progress of radiation induced atherosclerosis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):493.[doi:10.3760/cma.j.issn.1673-4114.2015.06.012]
[18]张俊伶,路璐,李德冠,等.基因芯片表达分析4Gyγ射线对小鼠骨髓c-kit阳性细胞影响[J].国际放射医学核医学杂志,2015,39(2):116.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
 Zhang Junling,Lu Lu,Li Deguan,et al.Expression changes of genes in c-kit positive cells after 4Gy γ-ray irradiation: agene chip analysis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):116.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
[19]范美婷,马云,何淑雅.耐辐射奇球菌中多效蛋白PprA功能的研究进展[J].国际放射医学核医学杂志,2015,39(2):149.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
 Fan Meiting,Ma Yun,He Shuya.Research progress and prediction on pleiotropic protein PprA from Deinococcus radiodurans[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(2):149.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
[20]李敏,贺欣,周则卫,等.BTG2作为一种新的电离辐射诱导基因的研究[J].国际放射医学核医学杂志,2014,38(2):71.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]
 Li Min,He Xin,Zhou Zewei,et al.Study on B-cell translocation gene 2 as a novel radiation-induced gene[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(2):71.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]

备注/Memo

备注/Memo:
收稿日期:2016-12-31。
基金项目:国家自然科学基金(31570850、81273001)
通讯作者:邵春林,Email:clshao@shmu.edu.cn
更新日期/Last Update: 2017-04-24