[1]李敏,贺欣,周则卫,等.BTG2作为一种新的电离辐射诱导基因的研究[J].国际放射医学核医学杂志,2014,38(2):71-74.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]
　Li Min,He Xin,Zhou Zewei,et al.Study on B-cell translocation gene 2 as a novel radiation-induced gene[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(2):71-74.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]
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BTG2作为一种新的电离辐射诱导基因的研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
38
期数:
2014年第2期
页码:
71-74
栏目:
出版日期:
2014-03-25

文章信息/Info

Title:
Study on B-cell translocation gene 2 as a novel radiation-induced gene
作者:
李敏1;  贺欣2;  周则卫2;  孟庆慧1;  樊赛军1; 2
1. 苏州大学医学部放射医学与防护学院肿瘤放射生物学实验室, 215123;
2. 中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室, 天津 300192
Author(s):
Li Min1;  He Xin2;  Zhou Zewei2;  Meng Qinghui1;  Fan Saijun1; 2
Laboratory of Tumor Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China
关键词:
肿瘤; 辐射; 电离; B细胞易位基因2
Keywords:
Cancer; Radiation; ionizing; B-cell translocation gene 2
DOI:
10.3760/cma.j.issn.1673-4114.2014.02.001
摘要:
目的 研究γ射线照射对肿瘤细胞的B细胞易位基因2(BTG2)表达水平的影响。方法 应用RT-PCR方法研究电离辐射对肿瘤细胞BTG2 mRNA水平的影响;应用Western blot方法测定电离辐射对肿瘤细胞BTG2蛋白表达水平的影响。结果 与未照射对照细胞相比,3 Gyγ射线单次剂量照射明显提高了人乳腺癌细胞系MCF-7、MDA-MB-231和人宫颈癌细胞系C33A、HeLa中的BTG2蛋白的表达水平。γ射线照射人乳腺癌细胞系MCF-7和MDA-MB-231导致了照射剂量和时间依赖性的BTG2表达水平的升高,而且这种改变既表现在BTG2 mRNA水平上,也反映在BTG2蛋白水平上。结论 BTG2是一种新的辐射诱导DNA损伤的反应基因,进一步研究将为BTG2作为肿瘤放射治疗的疗效和预后评价指标提供实验基础和依据。
Abstract:
Objective To investigate the effects of ionizing radiation on the expression of B-cell translocation gene 2 (BTG2)in cancer cells.Methods RT-PCR was used to determine the level of BTG2 mRNA,while the expression of BTG2 protein was assayed by Western blot.Results Compared with the unirradiated cells,a single dose of 3 Gy γ-rays caused a significant increase of BTG2 protein in breast cancer cell lines,MCF-7 and MDA-MB-231,and cervical cancer cell lines,C33A and HeLa.Furthermore,irradiation with γ-rays resulted in a dose and time dependent increase of BTG2 at both mRNA and protein levels.Conclusion The present study demonstrates,for the first time,that BTG2 is a value response gene to exposure of ionizing radiation,which may provide evidence for BTG2 as a novel marker for radiotherapy of cancer.

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备注/Memo

备注/Memo:
收稿日期:2014-03-12;改回日期:。
基金项目:国家自然科学基金(81071906,81172127)
通讯作者:樊赛军(Email:fansaijun@irm-cams.ac.cn)
更新日期/Last Update: 1900-01-01