[1]付岳,王彦,杜利清,等.sirt1基因沉默在辐射诱导的nlrp3和il-1β表达中的作用研究[J].国际放射医学核医学杂志,2015,39(5):367-370.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
　fu yue*,wang yan,du liqing,et al.effects of nlrp3 and il-1β on radiation-induced expression through sirt1 gene silencing[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):367-370.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第5期
页码:
367-370
栏目:
论著
出版日期:
2015-09-15

文章信息/Info

Title:
effects of nlrp3 and il-1β on radiation-induced expression through sirt1 gene silencing
作者:
付岳; 王彦; 杜利清; 徐畅; 刘建香; 樊飞跃; 苏旭; 樊赛军; 刘强
300192，中国医学科学院北京协和医学院放射医学研究所，天津市放射医学与分子核医学重点实验室（付岳，王彦，杜利清，徐畅，樊飞跃，樊赛军，刘强）；101111，北京市医疗器械检验所，医疗器械检验与安全性评价北京市重点实验室（付岳）；100088 北京，中国疾病预防控制中心辐射防护与核安全医学所（刘建香，苏旭）
Author(s):
fu yue*;  wang yan;  du liqing;  xu chang;  liu jianxiang;  fan feiyue;  su xu;  fan saijun;  liu Qiang.
*tianjin key laboratory of radiation medicine and molecular nuclear medicine, institute of radiation medicine, chinese academy of medical science, peking union medical college, tianjin 300192, china
关键词:
沉默信息调节蛋白质类; 白细胞介素1β; 辐射; 电离; 白藜芦醇; nod样受体蛋白3
Keywords:
silent information regulator proteins;  interleukin-1β;  radiation;  ionizing;  resveratrol;  nod-like receptor protein 3
DOI:
10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003
摘要:
目的 研究沉默信息调节因子1（sirt1）基因沉默对间充质干细胞（mscs）受照后nod样受体蛋白3（nlrp3）和il-1β表达的影响，探讨sirt1激活剂——白藜芦醇的辐射防护作用及其机理。 方法 将mscs分为空白对照组、单纯照射组、rna干扰组、白藜芦醇组和rna干扰+白藜芦醇组。采用酶联免疫吸附测定、western blot和rt-pcr等方法检测il-1β、sirt1和nlrp3在蛋白和mrna水平的表达。结果 辐射可导致mscs细胞外il-1β分泌水平明显升高，给予白藜芦醇后，细胞il-1β分泌水平较单纯照射组显著下降（t=21.68，p<0.01），nlrp3和il-1β mrna水平较单纯照射组明显降低（t=14.44，p<0.01；t=12.35，p<0.01），sirt1基因沉默后，nlrp3和il-1β的mrna水平回升至单纯照射组水平（t=14.86，p<0.01；t=11.12，p<0.01），即使再给予白藜芦醇，nlrp3和il-1β的mrna水平仍明显高于白藜芦醇组（t=11.31，p<0.01；t=10.54，p<0.01）。结论 sirt1基因沉默减弱了白藜芦醇对辐射诱导的nlrp3和il-1β的抑制作用，说明白藜芦醇可能通过激活sirt1、抑制nlrp3、降低il-1β的表达，从而减轻辐射引起的细胞损伤。
Abstract:
objective to investigate the effect of silence information regulator-1（sirt1） on nlrp3 and il-1β production in mesenchymal stem cells（mscs） exposed to radiation and to explore the sirt1 role against radiation. methods mscs were divided into control, irradiation, rnai, resveratrol, and rnai+resveratrol group. il-1β, sirt1, and nod-like receptor protein 3（nlrp3） expressions were detected using enzyme-linked immunosorbent assay（elisa）, western blot analysis, and real-time pcr assay. results extracellular il-1β secretion induced by radiation was increased significantly. after resveratrol treatment, the levels of il-1β secretion decreased compared with those of the radiation group（t=21.68, p<0.01). the mrna level of nlrp3 and il-1β also decreased compared with that of the radiation group（t=14.44, p<0.01; t=12.35, p<0.01）. sirt1 knockdown significantly suppressed resveratrol-mediated anti-inflammatory activity（t=14.86, p<0.01; t=11.12, p<0.01）. the mrna level of nlrp3 and il-1β remained higher in the rnai+resveratrol group than those in the resveratrol group （t=11.31, p<0.01; t=10.54, p<0.01）. conclusions sirt1 gene silencing can suppress nlrp3 and il-1β expression induced by radiation. thus, resveratrol may alleviate cellular damage induced by radiation through activation of sirt1, inhibition of nlrp3, and decrease in il-1β expression.

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备注/Memo

备注/Memo:
基金项目：卫生部卫生行业科研专项（201002009）；国家自然科学基金（31300695）；天津市自然科学基金（12jcybjc15300， 13jcQnjc11600）
通信作者：刘强（email：liuqiang@irm-cams.ac.cn）
更新日期/Last Update: 2015-09-15