[1]董娟聪,刘红艳,党旭红,等.12c重离子与γ射线对人外周血淋巴细胞peng-ebv增殖、细胞周期及凋亡影响的比较[J].国际放射医学核医学杂志,2015,39(5):385-388.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
　dong juancong,liu hongyan,dang xuhong,et al.effects of 12c heavy ion and γ ray irradiation in cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):385-388.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
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12c重离子与γ射线对人外周血淋巴细胞peng-ebv增殖、细胞周期及凋亡影响的比较(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第5期
页码:
385-388
栏目:
论著
出版日期:
2015-09-15

文章信息/Info

Title:
effects of 12c heavy ion and γ ray irradiation in cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes
作者:
董娟聪; 刘红艳; 党旭红; 原雅艺; 张慧芳; 王超; 段志凯; 左雅慧
030006 太原，中国辐射防护研究院放射医学室
Author(s):
dong juancong;  liu hongyan;  dang xuhong;  yuan yayi;  zhang huifang;  wang chao;  duan zhikai;  zuo yahui.
department of radiological medicine, china institute for radiation protection, taiyuan 030006, china
关键词:
重离子; γ射线; 淋巴细胞; 辐射; 生物效应
Keywords:
heavy ions;  gamma rays;  lymphocytes;  radiation;  biological effect
DOI:
10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007
摘要:
目的 比较12c重离子与γ射线对人外周血淋巴细胞peng-ebv增殖抑制率、细胞周期和凋亡率的影响。方法　 对peng-ebv细胞分别进行2.0 gy的12c重离子和γ射线照射，通过mts法检测细胞增殖变化，流式细胞术检测细胞凋亡率和周期分布。结果　 2.0 gy重离子射线和γ射线作用后，peng-ebv细胞的增殖抑制率均显著升高。与对照组相比，2.0 gy射线照射后24、48、72 h细胞增殖抑制率差异有统计学意义（重离子射线：t=10.53、4.11、37.30，p<0.05；γ射线：t=7.18、8.26、26.11，p<0.05）。12c重离子对细胞的增殖抑制率在照射后72 h内呈时间依赖性升高，而γ射线则在照射后48 h时增殖抑制率最高，而后降低。重离子射线照射后12 h和24 h，细胞凋亡率分别较对照组增加了7.92和13.41倍（t=7.09、4.13，p<0.05）；而γ射线照射后12 h和24 h，细胞凋亡率分别为对照组的2.43和5.64倍（t=8.98、20.52，p<0.05）。重离子射线照射后12 h和24 h，细胞g2/m期阻滞程度分别较对照组增加5.11和11.77倍（t=12.06、100.08，p<0.05）；而γ射线照射后12 h和24 h，细胞g2/m期阻滞程度分别为对照组的5.82和7.68倍（t=19.35、10.30，p<0.05）。结论　 12c重离子对人外周血淋巴细胞造成的生物学效应远高于γ射线。
Abstract:
objective to compare the effects of carbon heavy ion and γ ray on cell survival, cell cycle and cell apoptosis. methods peng-ebv cells were irradiated by carbon heavy ion and x-ray. mts was used to detect the cell survival. cell cycle and apoptosis were analyzed by flow cytometry. results the proliferation inhibition rate of peng-ebv cell was significantly increased after 2.0 gy heavy ion rays and γ rays. compared with the control group, the cell proliferation inhibition rate difference was statistically significant after 2.0 gy rays at 24 h, 48 h, 72 h（heavy rays：t=10.53, 4.11, 37.30, all p<0.05; γ rays：t=7.18, 8.26, 26.11, all p<0.05）. the cell proliferation inhibition rate showed a time-dependent increase, and reached its peak at 72 h after heavy ion rays, while the cell proliferation inhibition rate after γ ray reached its peak at 48 h, and then decreased. compared with the control group, the apoptosis rate of peng-ebv cell increased 7.92 and 13.41 times respectively at 12 h and 24 h after 2.0 gy heavy ion rays（t=7.09, 4.13, both p<0.05）, while it was 2.43 and 5.64 times after 2.0 gy γ rays（t=8.98, 20.52, p<0.05）. compared with the control group, the g2/m phase arrest increased 5.11 and 11.77 times respectively at 12 h and 24 h after 2.0 gy heavy ion rays（t=12.06, 100.08, both p<0.05）, however, it was 5.82 and 7.68 times after 2.0 gy γ rays（t=19.35, 10.30, both p<0.05）. conclusion 12c heavy ion irradiation shows more stronger biological effects on human peripheral blood lymphocyte than γ ray.

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备注/Memo

备注/Memo:
通信作者：左雅慧（email：yahuiz@163. com）
更新日期/Last Update: 2015-09-15