[1]路璐,李德冠,王月英,等.脂多糖对受照小鼠血清IL-10、IL-6和TNF-α水平的影响[J].国际放射医学核医学杂志,2012,36(3):154-157.[doi:10.3760/cma.j.issn.1673-4114.2012.03.008]
　LU Lu,LI De-guan,WANG Yue-ying,et al.Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(3):154-157.[doi:10.3760/cma.j.issn.1673-4114.2012.03.008]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第3期
页码:
154-157
栏目:
放射生物学
出版日期:
2012-05-25

文章信息/Info

Title:
Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice
作者:
路璐;  李德冠;  王月英;  张俊玲;  吴红英;  孟爱民
中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津 300192
Author(s):
LU Lu;  LI De-guan;  WANG Yue-ying;  ZHANG Jun-ling;  WU Hong-ying;  MENG Ai-min
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
脂多糖类; γ射线; 白细胞介素10; 白细胞介素6; 肿瘤坏死因子α
Keywords:
Lipopolysaccharides; Gamma rays; Interleukin-10; Interleukin-6; Tumor necros is factor-alpha
DOI:
10.3760/cma.j.issn.1673-4114.2012.03.008
摘要:
目的 探讨6Gy 137Csγ射线一次性全身照射后,小鼠血清中白细胞介素10(IL-10)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)水平的变化及对脂多糖刺激反应性的远期影响。方法 将实验小鼠分为假照射组和照射组,假照射组不接受照射,照射组给予6Gy1射线照射。照射后10周,将假照射组和照射组小鼠按组别分别提前24h和1h腹腔注射脂多糖(20mg/kg),对照组注射生理盐水(100μl/只)。取小鼠外周血,利用酶联免疫吸附技术检测小鼠血清中IL-10、IL-6和TNF-α的水平。结果 假照射组小鼠在给予脂多糖刺激1h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=7.31、2.71和15.09,P分别为<0.01、<0.05和<0.01)。照射组小鼠在给予脂多糖刺激1h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=4.14、7.18和5.14,P均<0.01)。与假照射组相比,照射组小鼠在给予脂多糖刺激24h后,TNF.仅和IL-6水平无明显变化,IL-10水平升高了19.9%(t=2.84,P<0.05)。结论 经6Gy γ射线照射后10周,小鼠免疫调节功能尚未完全恢复;在接受脂多糖刺激后,假照射组和照射组小鼠的抗感染能力也有差异。脂多糖对辐射后小鼠血清中IL-10、IL-6和TNF-α水平的影响及其意义仍需进一步研究。
Abstract:
Objective To investigate the effects of lipopolysaccharide (LPS) on interleukin-10(IL-10), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels in mice exposed whole body to 6 Gy γ-ray radiation once. Methods The mice were divided into sham-irradiation group and irradiation group. Mice in sham-irradiation group accepted no irradiation, while mice in irradiation group accepted 6 Gy whole body irradiation at dose rate of 0.8 Gy/min. Mice in LPS treated group accepted intraperitoneal injection of LPS (20 mg/kg) at 10 weeks after irradiation, while mice in control group were accepted intraperitoneal injection of saline. The peripheral blood was collected by eyeballs enucleation after 1 h and 24 h respectively, then IL-10, IL-6 and TNF-α levels were detected by enzyme-linked immunoassay. Results Compared to control group, the levels of IL-10, IL-6 and TNF-α in blood serum increased at 1 h and 24 h after LPS injection both in sham-irradiation group (t=7.31, P<0.01; t=2.71, P<0.05; t=15.09, P<0.01) and irradiation group (t=4.14, 7.18 and 5.14, all P<0.01). Compared to sham-irradiation group, the level of IL-10 increased 19.9% at 24 h after LPS injection in irradiation group(t=2.84, P<0.05), while the levels of TNF-α and IL-6 had no significant differences between sham-irradiation group and irradiation group. Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy γ-ray irradiation. After LPS injection, there was difference of anti-infection ability between sham-irradiation group and irradiation group. The effects of LPS on cytokines IL-10, IL-6 and TNF-α levels in radiation-exposed mice need to be further explored.

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备注/Memo

备注/Memo:
收稿日期:2012-04-04。
基金项目:国家重点基础研究发展计划(2011CB964800-G);国家自然科学基金(81072237,81102873);天津市自然科学基金(12JCQNJC09100)
通讯作者:盂爱民(Email:ai_min_meng@126.com)
更新日期/Last Update: 1900-01-01