[1]王芹,刘晓秋,李进,等.IRM-2小鼠全长cDNA编码蛋白质分析[J].国际放射医学核医学杂志,2012,36(3):157-159.[doi:10.3760/cma.j.issn.1673-4114.2012.03.009]
　WANG Qin,LIU Xiao-qiu,LI Jin,et al.Analysis of proteins encoded by full-length cDNA sequence from IRM-2 mouse[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(3):157-159.[doi:10.3760/cma.j.issn.1673-4114.2012.03.009]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第3期
页码:
157-159
栏目:
放射生物学
出版日期:
2012-05-25

文章信息/Info

Title:
Analysis of proteins encoded by full-length cDNA sequence from IRM-2 mouse
作者:
王芹;  刘晓秋;  李进;  杜利清;  孙志娟;  王彦;  刘强;  宋力;  樊飞跃
北京协和医学院中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津 300192
Author(s):
WANG Qin;  LIU Xiao-qiu;  LI Jin;  DU Li-qing;  SUN Zhi-juan;  WANG Yah;  LIU Qiang;  SONG Li;  FAN Fei-yue
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medi-call Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
文库; 表达的序列标记; 数据库; 核酸; IBM-2小鼠
Keywords:
Library; Expressed sequence tags; Database; Nucleic acid; IRM-2 mice
DOI:
10.3760/cma.j.issn.1673-4114.2012.03.009
摘要:
目的 分离和鉴定IRM-2小鼠辐射抗性相关基因。方法 以IRM-2小鼠的21条表达序列标签为模板进行PCR扩增,从IRM-2小鼠全长cDNA文库中测定cDNA克隆的序列,通过与GenBank进行同源序列信息比对,对全长cDNA编码的蛋白质进行分析。结果 从IRM-2小鼠全长cDNA文库中获得5条全长cDNA序列,与小鼠已知基因不同源,得到了可能编码蛋白质的氨基酸序列和蛋白质的理化性质等信息。结论 通过对全长cDNA编码的蛋白质进行分析,提示IRM-2小鼠体内可能存在目前尚未发现的辐射抗性相关基因。
Abstract:
Objective To screen and isolate radioresistance-related genes from IRM-2 mouse. Methods Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tags. The property of proteins encoded by full-length cDNA were analyzed by comparing with GenBank database. Results Five pieces of full-length cDNA which were not the same source as the known mice genes were found out from IRM-2 mouse cDNA library. Amino acid sequence and property of proteins encoded by these five pieces of full-length cDNA were obtained. Conclusion Proteins encoded by full-length cDNA imply that unkown radioresistance-related genes may exist in IRM-2 mouse.

参考文献/References:

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[3] 王芹,李进,宋力,等.IRM-2小鼠全长cDNA文库的构建及鉴定.中华放射医学与防护杂志,2010(3):274-278.
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备注/Memo

备注/Memo:
收稿日期:2012-02-28。
基金项目:国家自然科学基金(30870583);中国医学科学院放射医学研究所学科发展基金(SF0822)
通讯作者:樊飞跃(Email:faithyfan@yahoo.com)
更新日期/Last Update: 1900-01-01