[1]李德冠,王月英,吴红英,等.大剂量γ射线照射对小鼠免疫系统损伤远期影响的研究[J].国际放射医学核医学杂志,2012,36(2):109-112.[doi:10.3760/cma.j.issn.1673-4114.2012.02.013]
　LI De-guan,WANG Yue-ying,WU Hong-ying,et al.The long-term effects of high γ-ray dose on the mice immune system[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):109-112.[doi:10.3760/cma.j.issn.1673-4114.2012.02.013]
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大剂量γ射线照射对小鼠免疫系统损伤远期影响的研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第2期
页码:
109-112
栏目:
放射生物学
出版日期:
2012-03-25

文章信息/Info

Title:
The long-term effects of high γ-ray dose on the mice immune system
作者:
李德冠;  王月英;  吴红英;  路璐;  王小春;  张恒;  樊飞跃;  孟爱民
中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津 300192
Author(s):
LI De-guan;  WANG Yue-ying;  WU Hong-ying;  LU Lu;  WANG Xiao-chun;  ZHANG Heng;  FAN Fei-yue;  MENG Ai-min
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
γ射线; 辐射损伤; 免疫系统; 小鼠; 近交C57BL; 脂多糖类
Keywords:
Gamma rays; Radiation injuries; Immune system; Mice;  inbred C57BL; Lipopolysaccharides
DOI:
10.3760/cma.j.issn.1673-4114.2012.02.013
摘要:
目的 探讨亚致死剂量6Gyγ射线一次性全身照射小鼠免疫细胞对脂多糖刺激反应性的远期影响.方法 将实验小鼠分为假照射组和照射组,假照射组不接受照射,照射组给予6 Gyγ射线照射.照射后10周,分别将假照射组和照射组小鼠按组别腹腔注射脂多糖(20 mg/kg),对照组注射等量的生理盐水.分别于24 h和1 h后取材,进行外周血白细胞计数及CD4、CD8、B220细胞比例检测.取脾脏和胸腺称重,计算脏器指数.冲洗单侧股骨进行有核细胞计数.结果 与假照射刘照组小鼠相比,照射对照组小鼠的CD4细胞比例显著升高(t=2.940,P<0.05),CD8和B220细胞比例显著下降(t=6.485和4.351,P均<0.01).照射+脂多糖1h组小鼠的CD4 (t=2.510,P<0.05)、CD8(t=2.862,P<0.05)和B220(t=7.074,P<0.01)细胞比例较假照射+脂多糖1h组小鼠显著降低,差异有统计学意义.与假照射+脂多糖24 h组小鼠相比,照射+脂多糖24 h组小鼠单侧股骨有核细胞计数显著升高,差异有统计学意义(t=2.078,P<0.05).结论 6 Gyγ射线一次性全身照射后10周,小鼠免疫系统尚未完全恢复;与假照射组相比,在接受脂多糖刺激后照射组小鼠胸腺指数和外周血中CD8和B220细胞比例未见显著变化.辐射对小鼠免疫系统损伤的远期影响有待进一步研究。
Abstract:
Objective To investigate the long-term effects of high γ-ray dose on the mice immune system to stimulation of lipopolysaccharide (LPS).Methods The mice were randomly divided into shamirradiation group and irradiation group.Mice in sham-irradiation group received no irradiation,while mice in irradiation group received 6 Gy γ-ray irradiation.The mice were accepted intraperitoneal injection of LPS (20 mg/kg) in LPS treated group at 10 weeks after irradiation,while the mice in control group were accepted intraperitoneal injection of saline.The mice were sacrificed after 1 h and 24 h respectively.The white blood cells,CD4,CD8 and B220 cells in peripheral blood were detected.The index of spleen and thymus were calculated and the number of bone marrow nucleated cells per femur were counted.Results Compared to the mice in sham-irradiation-control group,the ratio of CD4 cells in irradiation-control group increased (t=2.940,P<0.05),while the CD8 and B220 cells decreased (t=6.485 and 4.351,both P<0.01).Compared to the mice in sham-irradiation-LPS 1h group,the ratio of CD4 (t=2.510,P<0.05),CD8 (t=2.862,P<0.05) and B220 (t=7.074,P<0.01) cells in irradiation-LPS 1 h group significantly decreased.Compared to the mice in sham-irradiation-LPS 24 h group,the number of bone marrow nucleated cells in irradiation-LPS 24h group significantly increased (t=2.078,P<0.05).Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy γ-ray irradiation.After the stimulation of LPS,the changes of thymus index and CD8,B220 cells numbers were less in the mice of irradiation group than the sham-irradiation group.The long-term effects of radiation on the mice immune system need to be further explored.

参考文献/References:

[1] Wang Y, Liu L, Pazhanisamy SK, et al. Total body irradiation causes resi dual bone marrow injury by induction of persistent oxidativestress in murine hematopoietic stem cells. Free Radic Biol Med, 2010, 48(2):348-356.
[2] 崔玉芳,丁彦青,徐菡,等.大剂量γ线照射对小鼠免疫功能近期、远期的影响.细胞与分子免疫学杂志,2004,20(6):675-677.
[3] Lu J, Guan S, Shen X, et al. Immunosuppressive activity of 8-gin-gerol on immune responses in mice. Molecules, 2011, 22, 16(3):2636-2645.
[4] 路璐,王月英,李德冠,等.LPS对辐射暴露后小鼠血清IL-10、IL-6和TNF-α水平的影响.中国辐射卫生,2010,19(3):265-266.
[5] Pecaut MJ, Dutta-Roy R, Miller GM, et al. Radiation and primary immune response to lipopolysaccharide:lymphocyte distribution and function. In Vivo, 2007, 21(3):463-470.
[6] Pecaut MJ, Gridley DS. Radiation and secondary immune response to lipopolysaccharide. In Vivo, 2008, 22(4):423-434.
[7] 李德冠,王月英,路璐,等.辐射对小鼠免疫系统损伤远期影响的研究.细胞与分子免疫学杂志,2011,27(12):1362-1363.
[8] 习忻,刘韩英,徐先早,等.五鹤续断对辐射损伤小鼠保护作用的研究.国际放射医学核医学杂志,2010(2):116-119.

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备注/Memo

备注/Memo:
收稿日期:2012-02-10。
基金项目:国家重点基础研究发展计划(2011CB964800-G)国家自然科学基金(81072237,81102873)天津市自然科学基金(12JCQNJC09100)
通讯作者:孟爱民,Email:ai_min_meng@126.com
更新日期/Last Update: 1900-01-01