[1]王小春,刘金剑,王月英,等.SKP2表达对食管癌细胞辐射敏感性的影响[J].国际放射医学核医学杂志,2012,36(2):105-108.[doi:10.3760/cma.j.issn.1673-4114.2012.02.012]
　WANG Xiao-chun,LIU Jin-Jian,WANG Yue-ying,et al.Effects of SKP2 expression on radio-sensitivity of esophageal carcinoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):105-108.[doi:10.3760/cma.j.issn.1673-4114.2012.02.012]
点击复制

SKP2表达对食管癌细胞辐射敏感性的影响(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第2期
页码:
105-108
栏目:
放射生物学
出版日期:
2012-03-25

文章信息/Info

Title:
Effects of SKP2 expression on radio-sensitivity of esophageal carcinoma
作者:
王小春;  刘金剑;  王月英;  吴红英;  李德冠;  褚丽萍;  刘强;  宋娜玲;  樊飞跃;  孟爱民
中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津 300192
Author(s):
WANG Xiao-chun;  LIU Jin-Jian;  WANG Yue-ying;  WU Hong-ying;  LI De-guan;  CHU Li-ping;  LIU Qiang;  SONG Na-ling;  FAN Fei-yue;  MENG Ai-min
Tianjin Key Laboratory of Molecalar Naclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China
关键词:
食管肿瘤; S期激酶相关蛋白质类; RNA干扰; 辐射耐受性
Keywords:
Esophageal neoplasms; S-phaes kinase-associated proteins; RNA interference; Radiation tolerance
DOI:
10.3760/cma.j.issn.1673-4114.2012.02.012
摘要:
目的 探讨S期激酶相关蛋白2(SKP2)高表达和低表达对食管癌细胞辐射敏感性的影响.方法 用Western-blotting方法筛选SKP2低表达和高表达的食管癌细胞系;构建SKP2正义表达载体p-pcDNATM3.1/myc-His A-SKP2和RNA干扰载体SKP2 RNAi,分别转染SKP2低表达和高表达的食管癌细胞系,用5 Gyγ射线照射各组细胞,通过克隆形成实验检测细胞辐射敏感性的差异.结果 SKP2在4种食管癌细胞中的表达水平依次为KYSE510 > KYSE450 > EC9706 > KYSE150.用p-pcDNATM 3.1/myc-His A-SKP2表达载体转染KYSE 150细胞后SKP2表达水平升高,5 Gyγ射线照射后细胞的克隆形成能力显著高于对照组(F=3.53,P<0.01),表明SKP2高表达可以降低食管癌细胞的辐射敏感性.RNA干扰载体转染KYSE510细胞后SKP2表达水平降低,5 Gyγ射线照射后细胞的克隆形成能力显著低于对照组(F=5.23,P<0.05),表明敲降SKP2表达可以增加食管癌细胞的辐射敏感性.结论 SKP2表达与食管癌细胞的辐射敏感性密切相关,具体机制还需要进一步研究。
Abstract:
Objective To explore the correlation of S-phase kinase-associated protein 2 (SKP2) expression with radio-sensitivity of esophageal carcinoma.Methods Detecting the level of SKP2 in four different esophageal carcinoma cell lines using Western-blotting.Constructing p-pcDNATM 3.1/myc-His A-SKP2 exprcssion and SKP2 RNAi vector and transfecting SKP2 low or high expression cell,respectively.Clone forming assay was used to detect the cell proliferation ability of different groups after 5 Gy γ-ray irradiation.Results The sequence of SKP2 expression level in four different esophageal carcinoma cell line was KYSES10 > KYSE450 > EC9706 > KYSE150.Compared with parent control cells,the clone forming ability of SKP2 high expression cells was significantly increased (F=3.53,P<0.01),showing high SKP2 expression depression the radio-sensitivity of KYSE 150 cell.In contrast,the clone forming ability of KYSE510 cell was significantly decreased after SKP2 knock-down (F=5.23,P<0.05),showing low SKP2 expression promoting the radio-sensitivity of KYSE510 cell.Conclusion SKP2 expression was correlated with radio-sensitivity of esophageal carcinoma,but further study was needed to explore the molecular mechanism.

参考文献/References:

[1] Zhang H, Kobayashi R, Galaktionov K, et al. pl9Skpl and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase. Cell, 1995, 82(6):915-925.
[2] Demetrick D J, Zhang H, Beach DH. Chromosomal mapping of the genes for the human CDK2/eyclin A-associated proteins p19(SKP 1A and SKP1 B) and p45(SKP2). Cytogenet Cell Genet, 1996, 73(1-2):104-107.
[3] Wang Z, Gao D, Fukushima H, et al. Skp2:A novel potential thera-peutic target for prostate cancer. Biochim Biophys Acta, 2012, 1825(1):11-17.
[4] Lin DC, Du XL, Wang MR. Protein alterations in ESCC and clinical implications:a review. Dis Esophagus, 2009, 22(1):9-20.
[5] Gstaiger M, Jordan R, Lim M, et al. Skp2 is oncogenic and overex-pressed in human cancers. Proc Natl Acad Sci USA, 2001, 98(9):5043-5048.
[6] Yokoi S, Yasui K, Saito-Ohara F, et al. A novel target genes SKP2, within the 5p13 amplicon that is frequently detected in small cell lung cancers. Am J Pathol, 2002, 161(1):207-216.
[7] Arbini AA, Greco M, Yao JL, et al. Skp2 overexpression is associ-ated with loss of BRCA2 protein in human prostate cancer. Am J Pathol, 2011, 178(5):2367-2376.
[8] Wang Z, Fukushima H, Inuzuka H, et al. Skp2 is a promising thera-peutic target in breast cancer. Front Oncol, 2012, 1(57):pii 18702.
[9] Ma XM, Liu Y, Guo JW, et al. Relation of overexpression of S phase kinase-associated protein 2 with reduced expression of p27 and PTEN in human gastric carcinoma. World J Gastroenterol, 2005, 11(42):6716-6721.
[10] Fujita T, Liu W, Doihara H, et al. Regulation of Skp2-p27 axis by the Cdhl/anaphase-promoting complex pathway in colorectal tumorigenesis. Am J Pathol, 2008, 173(1):217-228.

相似文献/References:

[1]李菲,黄俊星,张俊.18F-FDG PET/CT在食管癌中的临床应用[J].国际放射医学核医学杂志,2016,40(4):282.[doi:10.3760/cma.j.issn.1673-4114.2016.04.009]
　Li Fei,Huang Junxing,Zhang Jun.The clinical application of 18F-FDG PET/CT in esophageal cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):282.[doi:10.3760/cma.j.issn.1673-4114.2016.04.009]
[2]李生栩,唐明灯,林端瑜,等.18F-FDG PET/CT在胸段食管鳞癌淋巴结转移中的诊断价值[J].国际放射医学核医学杂志,2016,40(6):408.[doi:10.3760/cma.j.issn.1673-4114.2016.06.002]
　Li Shengxu,Tang Mingdeng,Lin Duanyu,et al.Value of 18F-FDG PET/CT in detecting metastatic lymph nodes of thoracic esophageal squamous cell carcinoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):408.[doi:10.3760/cma.j.issn.1673-4114.2016.06.002]
[3]谢彦婷,郑容.18F-FDG PET/CT在食管癌放疗中的应用[J].国际放射医学核医学杂志,2013,37(1):42.[doi:10.3760/cma.j.issn.1673-4114.2013.01.012]
　XIE Yan-ting,ZHENG Rong.The application of 18F-FDG PET/CT in the radiotherapy of esophageal cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(2):42.[doi:10.3760/cma.j.issn.1673-4114.2013.01.012]
[4]张敏,周莉,张开贤,等.中下段食管癌调强放疗布野方案剂量学比较[J].国际放射医学核医学杂志,2012,36(2):121.[doi:10.3760/cma.j.issn.1673-4114.2012.02.016]
　ZHANG Min,ZHOU Li,ZHANG Kai-xian,et al.Dosimetric comparison of different schemes for arrange beams in intensity modulated radiation therapy for mid-and distal-esophageal carcinoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):121.[doi:10.3760/cma.j.issn.1673-4114.2012.02.016]
[5]王月英,吴红英,李德冠,等.小鼠不同肿瘤模型对放化疗敏感性的研究[J].国际放射医学核医学杂志,2012,36(5):289.[doi:10.3760/cnla.j.issn.1673-4114.2012.05.006]
　WANG Yue-ying,WU Hong-ying,LI De-guan,et al.The radiation sensitivities of different mice tumor model to radiotherapy and chemotherapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):289.[doi:10.3760/cnla.j.issn.1673-4114.2012.05.006]
[6]陈凤华,李进,谭志军,等.Ad-Rb94联合γ射线照射对食管癌细胞生长的影响[J].国际放射医学核医学杂志,2010,34(2):119.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
　CHEN Feng-hua,LI Jin,TAN Zhi-jun,et al.Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γ-ray on growth of esophageal carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):119.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
[7]王珍芳,万卫星.食管癌中18F-氟脱氧葡萄糖摄取和葡萄糖转运蛋白1表达的相关性研究[J].国际放射医学核医学杂志,2009,33(1):18.[doi:10.3760/cma.j.issn.1673-4114.2009.01.018]
　WANG Zhen-fang,WAN Wei-xing.Research of the relationship between expression of glucose transporter-1 and 18F-fluorodeoxyglucose uptake in esophgeal cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2009,33(2):18.[doi:10.3760/cma.j.issn.1673-4114.2009.01.018]
[8]杨吉刚,李春林.18F-氟脱氧葡萄糖PET及PET-CT在食管癌中的[J].国际放射医学核医学杂志,2007,31(1):31.
　YANG Ji-gang,LI Chun-lin.Application of 18F-fluorodeoxyglucose PET and PET-CT imaging in esophageal cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):31.
[9]郁春景,万卫星.18F-氟脱氧葡萄糖PET、超声内镜及CT在食管癌术前分期中的应用[J].国际放射医学核医学杂志,2007,31(3):144.
　YU Chun-jing,WAN Wei-xing.18F-fluorodeoxyglucose PET, endoscopic ultrasound and CT in the initial staging of patients with esophageal cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):144.
[10]刘军叶,郭鹞,郭国祯.Bcl-2基因特异性小干涉RNA增强食管癌细胞辐射敏感性的实验研究[J].国际放射医学核医学杂志,2006,30(2):110.
　LIU Jun-ye,GUO Yao,GUO Guo-zhen.Small interfering RNA specific to Bcl-2 enhances radiosensitivity of oesophageal cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(2):110.

备注/Memo

备注/Memo:
收稿日期:2012-02-04。
基金项目:国家自然科学基金(30901723)天津市自然科学基金(11JCYBJC13700)中国医学科学院放射医学研究所学科发展基金(SF1103,1106)
通讯作者:王小春,Email:wxc3188@126.com
更新日期/Last Update: 1900-01-01