[1]陈雪梅,周树云,范源,等.表没食子儿茶素没食子酸酯对131I辐射损伤所致甲减大鼠模型抗氧化体系的保护作用[J].国际放射医学核医学杂志,2015,39(4):311-315.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
　Chen Xuemei,Zhou Shuyun,Fan Yuan,et al.Protection of antioxidant system of EGCG on the thyroid in rat model from 131I radiation damage[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):311-315.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
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表没食子儿茶素没食子酸酯对131I辐射损伤所致甲减大鼠模型抗氧化体系的保护作用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第4期
页码:
311-315
栏目:
出版日期:
2015-07-25

文章信息/Info

Title:
Protection of antioxidant system of EGCG on the thyroid in rat model from 131I radiation damage
作者:
陈雪梅1;  周树云2;  范源3;  瞿博2
1. 云南中医学院药学院药学专业, 昆明, 650500;
2. 云南省中西医结合医院内科, 昆明, 650500;
3. 云南中医学院第一附属医院内分泌科, 昆明, 650500
Author(s):
Chen Xuemei1;  Zhou Shuyun2;  Fan Yuan3;  Qu Bo2
Specialty of Pharmaceutical, Yunnan University of TCM, School of Pharmacy, Kunming 650500, China
关键词:
碘放射性同位素; 辐射损伤; 甲状腺功能减退症; 表没食子儿茶素没食子酸酯; 抗氧化体系
Keywords:
Iodine radioisotopes; Radiation injuries; Hypothyroidism; Epigallocatechin gallate; Antioxidant system
DOI:
10.3760/cma.j.issn.1673-4114.2015.04.009
摘要:
目的 探讨不同剂量表没食子儿茶素没食子酸酯(EGCG)对131I辐射损伤所致甲状腺功能减退症(甲减)大鼠抗氧化体系的保护作用。方法 3月龄健康SD大鼠52只,随机分成6组,即空白组、模型组、甲状腺片组、EGCG低剂量组(25 mg·kg-1·d-1)、EGCG中剂量组(50 mg·kg-1·d-1)、EGCG高剂量组(100 mg·kg-1·d-1)。除空白组外,其余5组给予131I灌胃2周以造甲减大鼠模型,造模成功后,空白组、模型组给予生理盐水,其他组给予相应的药物。4周后,利用生物化学方法测定大鼠血清中游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、TSH水平,同时测定超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)的含量。结果 与模型组相比,甲状腺片组、EGCG组大鼠血清FT3、FT4水平均有升高,甲状腺片组、EGCG高剂量组TSH水平明显降低,EGCG低剂量组、EGCG中剂量组差异无统计学意义;与模型组相比,甲状腺片组、EGCG组的SOD水平明显降低,GSH-Px水平明显升高;EGCG中、高剂量组CAT水平显著升高。结论 EGCG能减轻131I辐射对大鼠甲状腺组织的氧化损伤,可保护大鼠抗氧化体系。
Abstract:
Objective To investigate the protection of different doses of epigallocatechin gallate (EGCG) on 131I radiation-induced thyroid dysfunction in rat antioxidant system.Methods A total of 52 3-month-old SD rats were randomly divided into six groups,namely,control,model,Euthyrox,EGCG low-dose (25 mg·kg-1·d-1),EGCG medium-dose (50 mg·kg-1·d-1),and EGCG high-dose (100 mg·kg-1·d-1) groups.Except for the control group,131I was intragastrically administered for 2 weeks to the five remaining groups to obtain hypothyroid rats.After the hypothyroid rat model was successfully established,the control and model groups received normal saline,whereas the other groups were treated with appropriate medication.After 4 weeks,the levels of serum-free triiodothyronine three (FT3),free thyroxine (FT4),and TSH were detected using a biochemical method.The levels of superoxide dismutase,glutathione peroxidase,and peroxidase were also determined.Results The Euthyrox and EGCG groups exhibited significantly elevated serum FT3 and FT4 levels compared with the model group.The TSH levels of the Euthyrox and EGCG high-dose groups were significantly reduced.No statistical significance was found in the EGCG low-and middle-dose groups.The superoxide dismutase activity of the Euthyrox and EGCG groups were significantly lower compared with that of the model group,whereas the glutathione peroxidase activity was significantly higher.The catalase levels were also significantly elevated in EGCG medium-and high-dose groups.Conclusion EGCG reduced the effect of radiation on the 131I oxidative thyroid tissue damage and protected the rat antioxidant system.

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备注/Memo

备注/Memo:
收稿日期:2015-01-15。
通讯作者:范源,Email:1647909799@qq.com;瞿博,Email:1061508680@qq.com
更新日期/Last Update: 1900-01-01