[1]刘建功,党旭红,刘红艳,等.核工业某厂放射工作人员与非放射工作人员的mdm2基因表达调查与分析[J].国际放射医学核医学杂志,2015,39(5):416-419.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 015]
　liu jiangong,dang xuhong,liu hongyan,et al.investigation and analysis of mdm2 expression level in radiation-exposed workers and non-radiation-exposed workers[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):416-419.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 015]
点击复制

核工业某厂放射工作人员与非放射工作人员的mdm2基因表达调查与分析(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第5期
页码:
416-419
栏目:
论著
出版日期:
2015-09-15

文章信息/Info

Title:
investigation and analysis of mdm2 expression level in radiation-exposed workers and non-radiation-exposed workers
作者:
刘建功; 党旭红; 刘红艳; 张忠新; 段志凯; 左雅慧
030006　太原，中国辐射防护研究院放射医学与环境医学研究所放射效应研究室
Author(s):
liu jiangong;  dang xuhong;  liu hongyan;  zhang zhongxin;  duan zhikai; zuo yahui.
division of radiological medicine, institute of radiation medicine and environmental medicine, china institute for radiation protection, taiyuan 030006, china
关键词:
辐射; 电离; 基因表达; 健康调查; mdm2基因; 外周血
Keywords:
radiation;  ionizing;  gene expression;  health surveys;  murine double minute 2 gene;  peripheral blood
DOI:
10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 015
摘要:
目的 探讨核工业某厂放射工作人员与非放射工作人员辐射敏感基因表达的差异。方法 采集了3名健康人外周血进行60co γ射线照射，建立辐照后鼠双微体基因2（mdm2）表达的剂量-效应关系，抽取核工业某厂96名工作人员（49名放射工作人员、47名非放射工作人员）外周血，利用实时荧光定量pcr技术检测放射工作人员外周血中mdm2基因的表达情况。结果 在0～2 gy剂量范围内，mdm2的表达水平随照射剂量增加而上升。某厂96名工作人员外周血mdm2基因表达水平的检测分析结果表明，非放射工作人员年龄因素对外周血mdm2基因表达无影响（f=2.11，p>0.05）；放射工作人员外周血mdm2基因表达与非放射工作人员相比，差异具有统计学意义（t=7.78，p<0.05）。结论 电离辐射可以诱导人离体外周血mdm2基因表达改变，且基因表达与照射剂量存在一定的剂量相关性，mdm2有望成为核工业放射工作人员健康调查的敏感指标。
Abstract:
objective to investigate the difference of radiation-sensitive gene expression between radiation-exposed and non-radiation-exposed workers in a nuclear industry factory. methods peripheral blood samples from 3 healthy volunteers were exposed to 60co γ-rays at different doses. exposure of murine double minute 2（mdm2） mrna expression to ionizing radiation was observed. mdm2 gene expression levels were detected in peripheral blood from 96 workers（i.e., 49 radiation-exposed workers and 47 non-radiation-exposed workers） in a nuclear industry factory by using real-time pcr. results the mdm2 expression levels of peripheral blood samples from 3 healthy volunteers exposed to 60co γ-rays increased with an increased dose of 0 gy to 2 gy. moreover, the relationship between the ratio of mdm2 mrna/β-actin and irradiation dose represents certain line correlation, which was fit by origin 7.5 software. the detection and analysis results of mdm2 gene expression levels in nuclear factory workers showed no statistically significant difference between different age groups and mdm2 gene expression levels in the peripheral blood of non-radiation-exposed equilibrium distribution of staff（f=2.11, p>0.05）. therefore, age factor had no effect on mdm2 gene expression. the mdm2 gene in the peripheral blood of radioactivity staff was statistically significant to radiation-exposed workers（t=7.78, p<0.05）. conclusions ionizing radiation can induce changes in gene expression from human peripheral blood, and this process is dose-related. mdm2 gene expression levels may be a new radiosensitive index in health examination survey in the future.

参考文献/References:

[1]周学平, 王红阳, 杨广顺, 等． mxr7基因的克隆及其在人正常和肿瘤组织中的表达[j]． 中华实验外科杂志, 1999, 16（2）：144-146．
[2]方宁波, 李勇． mxr7基因与原发性肝癌关系的研究进展[j]． 南昌大学学报：医学版, 2013, 53（5）：97-100．
[3]izetti p, hautefeuille a, abujamra al, et al. prima-1, a mutant p53 reactivator, induces apoptosis and enhances chemotherapeutic cytotoxicity in pancreatic cancer cell lines[j]. invest new drugs, 2014, 32（5）：783-794.
[4]tremosini s, forner a, boix l, et al. prospective validation of an immunohistochemical panel（glypican 3, heat shock protein 70 and glutamine synthetase） in liver biopsies for diagnosis of very early hepatocellular carcinoma[j]. gut, 2012, 61（10）：1481-1487.
[5]masuzaki r, karp sj, omata m. new serum markers of hepatocellular carcinoma[j]. semin oncol, 2012, 39（4）：434-439.
[6]tu jb, li qy, jiang f, et al. pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway[j]. mol med rep, 2014, 10（1）：301-305.
[7]gato we, mcgee sr, hales db, et al. time-dependent regulation of apoptosis by aen and bax in response to 2-aminoanthracene dietary consumption[j]. toxicol int, 2014, 21（1）：57-64.
[8]祝峙, 冯真, 张晶, 等． 生长抑制因子1剪接变异体调控p53信号通路诱导肝癌细胞凋亡[j]． 肿瘤基础与临床, 2013, 26（5）：369-375．

相似文献/References:

[1]徐畅,方连英,孔阳阳,等.沉默BLAP75基因对电离辐射诱导DNA损伤的影响[J].国际放射医学核医学杂志,2016,40(4):244.[doi:10.3760/cma.j.issn.1673-4114.2016.04.001]
　Xu Chang,Fang Lianying,Kong Yangyang,et al.Effects of silencing BLAP75 on DNA damage induced by ionizing radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):244.[doi:10.3760/cma.j.issn.1673-4114.2016.04.001]
[2]董娟聪,刘红艳,党旭红,等.12c重离子与γ射线对人外周血淋巴细胞peng-ebv增殖、细胞周期及凋亡影响的比较[J].国际放射医学核医学杂志,2015,39(5):385.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
　dong juancong,liu hongyan,dang xuhong,et al.effects of 12c heavy ion and γ ray irradiation in cell growth, cell cycle and apoptosis of human peripheral blood lymphocytes[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):385.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 007]
[3]李晴,王璐,徐畅,等.姜油树脂对辐射后间充质干细胞nrf2及其靶基因表达的影响[J].国际放射医学核医学杂志,2015,39(5):389.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 008]
　li Qing,wang lu,xu chang,et al.ginger oleoresin induced expression of nrf2 and its target genes in mesenchymal stem cells after radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):389.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 008]
[4]徐金苹,袁德晓,张江虹,等.辐射诱导的外泌体在肿瘤细胞侵袭转移中的作用[J].国际放射医学核医学杂志,2015,39(2):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]
　Xu Jinping,Yuan Dexiao,Zhang Jianghong,et al.The role of radiation-induced exosomes in tumor invasion and metastasis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]
[5]王芹,王敬敏,徐畅,等.沉默XRCC2基因表达联合电离辐射对结肠癌细胞增殖能力的影响[J].国际放射医学核医学杂志,2015,39(4):282.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
　Wang Qin,Wang Jingmin,Xu Chang,et al.The effect of silencing XRCC2 gene combined with ionizing radiation on growth of colorectal cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):282.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
[6]施常备,许建林,袁勇,等.染色体畸变率估算32P的辐射剂量[J].国际放射医学核医学杂志,2012,36(1):35.[doi:10.3760/cma.j.issn.1673-4114.2012.01.009]
　SHI Chang-bei,XU Jian-lin,YUAN Yong,et al.The quantitative radiation dose of 32P by chromosomal aberration[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):35.[doi:10.3760/cma.j.issn.1673-4114.2012.01.009]
[7]赵欣然,江波,傅宝华,等.《过量照射人员的医学检查与处理原则》解读[J].国际放射医学核医学杂志,2012,36(4):207.[doi:10.3760/cma.j.issn.1673-4114.2012.04.003]
　ZHAO Xin-ran,JIANG Bo,FU Bao-hua,et al.Explanation of Medical Examination and Management Principle for the Overexposed Individuals[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):207.[doi:10.3760/cma.j.issn.1673-4114.2012.04.003]
[8]刘玉龙,姜忠.《核事故场内医学应急响应程序》解读[J].国际放射医学核医学杂志,2012,36(4):218.[doi:10.3760/cma.j.issn.1673-4114.2012.04.006]
　LIU Yu-long,JIANG Zhong.Explanation of Procedure on Site Medical Emergency Response for Nuclear Accident[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):218.[doi:10.3760/cma.j.issn.1673-4114.2012.04.006]
[9]刘强,王彦,杜利清,等.《单细胞凝胶电泳用于受照人员剂量估算技术规范》解读[J].国际放射医学核医学杂志,2012,36(4):242.[doi:10.3760/cma.j.issn.1673-4114.2012.04.012]
　LIU Qiang,WANG Yan,DU Li-qing,et al.Explanation for the Specification of Dose Estimation for the Radiation Victims in the Early Stage Using Single Cell Gel Electrophoresis[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):242.[doi:10.3760/cma.j.issn.1673-4114.2012.04.012]
[10]刘强,王彦.ICRP118关于辐射诱导组织反应内容的部分修改建议[J].国际放射医学核医学杂志,2013,37(6):389.[doi:10.3760/cma.j.issn.1673-4114.2013.06.016]
　LIU Qiang,WANG Yan.Revision recommendations of tissue reaction induced by radiation in ICRP118[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):389.[doi:10.3760/cma.j.issn.1673-4114.2013.06.016]
[11]张宇睿,徐文清.电离辐射对线粒体损伤的研究进展[J].国际放射医学核医学杂志,2016,40(2):154.[doi:10.3760/cma.j.issn.1673-4114.2016.02.014]
　Zhang Yurui,Xu Wenqing.Damages of ionizing radiation on mitochondria[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):154.[doi:10.3760/cma.j.issn.1673-4114.2016.02.014]
[12]张俊伶,薛晓蕾,李源,等.富氢水对电离辐射引起胸腺细胞损伤的影响[J].国际放射医学核医学杂志,2015,39(5):358.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 001]
　zhang junling,xue xiaolei,li yuan,et al.effects of hydrogen-rich water on radiation-induced thymus injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):358.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 001]
[13]付岳,王彦,杜利清,等.sirt1基因沉默在辐射诱导的nlrp3和il-1β表达中的作用研究[J].国际放射医学核医学杂志,2015,39(5):367.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
　fu yue*,wang yan,du liqing,et al.effects of nlrp3 and il-1β on radiation-induced expression through sirt1 gene silencing[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):367.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 003]
[14]王璐,李晴,徐畅,等.sirna干扰sirt1对辐射后il-6表达的影响[J].国际放射医学核医学杂志,2015,39(5):423.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
　wang lu,li Qing,xu chang,et al.effect of sirt1 sirna interference on the expression of il-6 after radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):423.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
[15]张倩如,李海涛,田红旗.小分子辐射防护药物的研究进展[J].国际放射医学核医学杂志,2016,40(5):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
　Zhang Qianru,Li Haitao,Tian Hongqi.Small molecule compounds against radiation:research advances[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
[16]肖林林,金问森,张江虹,等.电离辐射诱发动脉粥样硬化的研究进展[J].国际放射医学核医学杂志,2015,39(6):493.[doi:10.3760/cma.j.issn.1673-4114.2015.06.012]
　Xiao Linlin,Jin Wensen,Zhang Jianghong,et al.Research progress of radiation induced atherosclerosis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):493.[doi:10.3760/cma.j.issn.1673-4114.2015.06.012]
[17]张俊伶,路璐,李德冠,等.基因芯片表达分析4Gyγ射线对小鼠骨髓c-kit阳性细胞影响[J].国际放射医学核医学杂志,2015,39(2):116.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
　Zhang Junling,Lu Lu,Li Deguan,et al.Expression changes of genes in c-kit positive cells after 4Gy γ-ray irradiation: agene chip analysis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):116.[doi:10.3760/cma.j.issn.1673-4114.2015.02.003]
[18]范美婷,马云,何淑雅.耐辐射奇球菌中多效蛋白PprA功能的研究进展[J].国际放射医学核医学杂志,2015,39(2):149.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
　Fan Meiting,Ma Yun,He Shuya.Research progress and prediction on pleiotropic protein PprA from Deinococcus radiodurans[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):149.[doi:10.3760/cma.j.issn.1673-4114.2015.02.010]
[19]李敏,贺欣,周则卫,等.BTG2作为一种新的电离辐射诱导基因的研究[J].国际放射医学核医学杂志,2014,38(2):71.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]
　Li Min,He Xin,Zhou Zewei,et al.Study on B-cell translocation gene 2 as a novel radiation-induced gene[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(5):71.[doi:10.3760/cma.j.issn.1673-4114.2014.02.001]
[20]韩英,杨冰,唐泉,等.电离辐射对原代成骨细胞M-CSF表达的影响[J].国际放射医学核医学杂志,2013,37(1):5.[doi:10.3760/cma.j.issn.1673-4114.2013.01.002]
　Han Ying,Yang Bing,Tang Quan,et al.Effects of radiation on macrophage colony stimulating factor in primary osteoblasts[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):5.[doi:10.3760/cma.j.issn.1673-4114.2013.01.002]

备注/Memo

备注/Memo:
通信作者：段志凯（email: duanzhikai@cirp. org. cn）
更新日期/Last Update: 2015-09-15