[1]王芹,王敬敏,徐畅,等.沉默XRCC2基因表达联合电离辐射对结肠癌细胞增殖能力的影响[J].国际放射医学核医学杂志,2015,39(4):282-286.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
　Wang Qin,Wang Jingmin,Xu Chang,et al.The effect of silencing XRCC2 gene combined with ionizing radiation on growth of colorectal cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):282-286.[doi:10.3760/cma.j.issn.1673-4114.2015.04.002]
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沉默XRCC2基因表达联合电离辐射对结肠癌细胞增殖能力的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第4期
页码:
282-286
栏目:
出版日期:
2015-07-25

文章信息/Info

Title:
The effect of silencing XRCC2 gene combined with ionizing radiation on growth of colorectal cancer cells
作者:
王芹1;  王敬敏2;  徐畅1;  杜利清1;  王彦1;  樊飞跃1;  刘强1
1. 中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室, 天津, 300192;
2. 保定市第二中心医院儿科, 保定, 072750
Author(s):
Wang Qin1;  Wang Jingmin2;  Xu Chang1;  Du Liqing1;  Wang Yan1;  Fan Feiyue1;  Liu Qiang1
Tianjin Key laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
辐射; 结肠肿瘤; RNA干扰; X射线修复交叉互补基因2
Keywords:
Radiation; Colonic neoplasms; RNA interference; X-ray repair cross complementing gene 2
DOI:
10.3760/cma.j.issn.1673-4114.2015.04.002
摘要:
目的 阐明shRNA干扰沉默X射线修复交叉互补基因2(XRCC2)对体外结肠癌T84细胞辐射敏感性的影响。方法 采用MTT法检测稳定表达XRCC2基因沉默的结肠癌T84细胞的生长并计算细胞生长抑制率,克隆形成实验检测经X射线照射后T84细胞的克隆形成能力,采用流式细胞术检测经X射线照射后T84细胞的细胞周期和细胞凋亡率。结果 shRNA-XRCC2组细胞于培养的第3天开始,增殖速度明显减慢,细胞生长抑制率平稳地维持在50%左右,明显低于shRNA-SC组(t=17.62、12.84、9.24,P<0.05)。shRNA-XRCC2组和8 Gy组的细胞克隆形成数目分别为422.7±43.4和389.5±24.4,shRNA-XRCC2+8 Gy组细胞克隆形成数最少(223.3±32.9),与shRNA-XRCC2组和8 Gy组相比,差异有统计学意义(t=8.96、9.92,P<0.01)。shRNA-XRCC2组停留在G2/M期的细胞增加(38.51±4.15)%,与对照组相比,差异有统计学意义(t=3.92,P<0.05);shRNA-XRCC2组细胞的凋亡率最高,达(33.16±2.69)%,与对照组相比,差异有统计学意义(t=15.31,P<0.01)。结论 XRCC2基因沉默有效地抑制了体外结肠癌T84细胞的增殖,XRCC2基因沉默联合辐射促使T84细胞阻滞在G2/M期并发生细胞凋亡,从而提高了T84细胞对辐射的敏感性。
Abstract:
Objective To investigate the effect of silencing X-ray repair cross complementary gene 2(XRCC2) through shRNA interference on the radiosensitivity of colorectal cancer cells.Methods The growth of colorectal cancer T84 cells of silencing XRCC2 was determined with MTT assay and the growth inhibition rate of T84 cells was detected.The ability to form colonies of T84 cells after exposure to X-ray radiation was examined with colony formation assay.The cell cycle distribution or cell apoptosis of T84 cells irradiated with X-ray radiation was performed by flow cytometric analysis.Results The cell growth of shRNA-XRCC2 group slowed down markedly from the third day of cell culture and the growth inhibition rate of shRNA-XRCC2 group steadily maintained about 50%,which was significantly less than that of shRNA-SC group (t=17.62,12.84,9.24,all P<0.05).The number of colonies formed in shRNA-XRCC2 cells and 8 Gy radiation cells was 422.7±43.4 and 389.5±24.4 respectively.The number of colonies in shRNA-XRCC2+8 Gy radiation group was the least (223.3±32.9),which was decreased significantly com-pared with that of shRNA-XRCC2 group or 8 Gy group (t=8.96 and 9.92,both P<0.01).Cells of shRNA-XRCC2 group arrested at G2/M phase were increased,reached (38.51±4.15)%,significantly higher than that of control group (t=3.92,P<0.05).Cells apoptosis ratio of shRNA-XRCC2 group cells was the highest,reached (33.16±2.69)%,and there were significant differences compared with that of control (t=15.31,P<0.01).Conclusion Knockdown of XRCC2 inhibited effectively the growth of colorectal cancer T84 cells in vitro.Silencing XRCC2 combined with radiation led to T84 cells arrested at G2/M phase and cells apoptosis and rendered T84 cells more sensitive to radiation.

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备注/Memo

备注/Memo:
收稿日期:2014-11-06。
基金项目:国家自然科学基金(项目编号:31170804);天津市自然科学基金(项目编号:12JCYBJC15300,12JCYBJC32900);北京协和医学院教学改革项目(项目编号:院1444)
通讯作者:刘强,Email:liuqiang@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01