[1]韩英,杨冰,唐泉,等.电离辐射对原代成骨细胞M-CSF表达的影响[J].国际放射医学核医学杂志,2013,37(1):5-8.[doi:10.3760/cma.j.issn.1673-4114.2013.01.002]
　Han Ying,Yang Bing,Tang Quan,et al.Effects of radiation on macrophage colony stimulating factor in primary osteoblasts[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(1):5-8.[doi:10.3760/cma.j.issn.1673-4114.2013.01.002]
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电离辐射对原代成骨细胞M-CSF表达的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第1期
页码:
5-8
栏目:
论著
出版日期:
2013-01-25

文章信息/Info

Title:
Effects of radiation on macrophage colony stimulating factor in primary osteoblasts
作者:
韩英1;  杨冰1;  唐泉1;  周慧2;  樊体强1;  樊飞跃1;  张晓东1;  孙元明1
1. 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津, 300192;
2. 天津医科大学药学院, 天津, 300070
Author(s):
Han Ying1;  Yang Bing1;  Tang Quan1;  Zhou Hui2;  Fan Ti-qiang1;  Fan Fei-yue1;  Zhang Xiao-dong1;  Sui Yuan-ming1
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
辐射; 电离; 成骨细胞; 破骨细胞; 放射疗法; 巨噬细胞集落刺激因子
Keywords:
Radiation; ionizing; Osteoblasts; Osteoclasts; Radiotherapy; Macrophage colony-stimulating factor
DOI:
10.3760/cma.j.issn.1673-4114.2013.01.002
摘要:
目的 研究辐射对于原代成骨细胞巨噬细胞集落刺激因子(M-CSF)表达的影响,进而研究辐射导致骨损伤的分子机理。方法 采用原代骨髓间充质干细胞诱导分化为成骨细胞,经0、2、4 Gy 137Cs γ射线照射后,采用实时定量PCR以及Western blot方法检测辐射对M-CSF mRNA和蛋白表达水平的影响。结果 2Gy和4Gyγ射线照射均能引起成骨细胞M-CSF mRNA(t=-17.329,P<0.01;t=-3.841,P<0.05)和蛋白表达水平上调。4 Gy照射后则会导致成骨前体细胞M-CSF mRNA表达水平上调(t=-4.478,P<0.05),但与对照组比较,两者的蛋白表达水平未见显着差异。结论 2 Gy和4Gyγ射线照射后,成骨细胞M-CSF表达水平上调能增强核因子κB受体活化因子配体对破骨细胞分化、成熟的促进作用,有助于增强破骨细胞的骨吸收能力。
Abstract:
Objective To investigate the radiation effect on the expression of macrophage colony stimulating factor(M-CSF)in primary osteoblasts and the molecular mechanism of bone injury induced by radiation.Methods Osteoblasts differentiated from bone marrow stromal cells (BMSCs)were analyzed by real-time PCR and Western blot after 2 Gy or 4 Gy 137Cs γ-irradiation.Results M-CSF mRNA and protein expression level were up-regulated after 2 Gy and 4 Gy irradiation (t=-17.329,P<0.01; t=-3.841,P<0.05)in osteoblasts.4 Gy irradiation increased M-CSF mRNA in osteoblast precursors (t=-4.478,P<0.05),but do not affect the protein expression level.Conclusions These results indicated that up-regulated M-CSF in osteoblasts could enhance the function of osteoclasts’ differentiation and maturation which induced by receptor activator of nuclear factor κB ligand after 2 Gy and 4 Gy irradiation.Further more,up-regulated M-CSF expression could enhance osteoclastic bone resorption of mature osteoclasts.

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备注/Memo

备注/Memo:
收稿日期:2012-09-09。
基金项目:国家自然科学基金(项目编号:30970867);中国医学科学院放射医学研究所学科发展基金(项目编号:SF1105/ST1223)
通讯作者:孙元明,Email:yuanmingsl962@163.com
更新日期/Last Update: 1900-01-01