[1]曹芳,张焕婷,刘强,等.50 Hz 1 mT脉冲电磁场对脐带间充质干细胞免疫调节能力的影响及其安全性评价[J].国际放射医学核医学杂志,2017,41(2):113-120.[doi:10.3760/cma.j.issn.1673-4114.2017.02.007]
　Cao Fang,Zhang Huanting,Du Liqing,et al.Effect and safety evaluation of 50-Hz 1-mT pulsed electromagnetic field on the immunomodulation of human umbilical cord mesenchymal stem cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2017,41(2):113-120.[doi:10.3760/cma.j.issn.1673-4114.2017.02.007]
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50 Hz 1 mT脉冲电磁场对脐带间充质干细胞免疫调节能力的影响及其安全性评价(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
41
期数:
2017年第2期
页码:
113-120
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Effect and safety evaluation of 50-Hz 1-mT pulsed electromagnetic field on the immunomodulation of human umbilical cord mesenchymal stem cells
作者:
曹芳1;  张焕婷1;  刘强2;  杜利清2;  曹晓沧1
1. 300052 天津, 天津医科大学总医院消化内科;
2. 300192 天津, 北京协和医学院中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室
Author(s):
Cao Fang1;  Zhang Huanting1;  Du Liqing2;  Liu Qiang2;  Cao Xiaocang1
1. Department of Gastroenterology and Hepatology, Tianjin Medical University General Hospital, Tianjin 300052, China;
2. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
间质干细胞; 辐射; 脉冲电磁场; 免疫调节
Keywords:
Mesenchymal stem cell; Radiation; Pulsed electromagnetic field; Immunomodulation
DOI:
10.3760/cma.j.issn.1673-4114.2017.02.007
摘要:
目的 探讨50 Hz 1 mT脉冲电磁场（PEMF）对脐带间充质干细胞（MSC）免疫调节能力的影响及其安全性。方法 采用50 Hz 1 mT PEMF辐照脐带MSC，MTT法检测辐照后细胞活性的变化；实时荧光定量PCR法检测脐带MSC的环氧合酶2（COX2）、IL-10、吲哚胺-2，3-双加氧酶（IDO）、肝细胞生长因子（HGF）和转化生长因子β（TGF-β）等基因mRNA表达水平的改变；流式细胞仪检测PEMF对脐带MSC活性氧（ROS）水平、细胞凋亡和细胞周期的影响；单细胞凝胶电泳分析脐带MSC DNA损伤的情况。采用SPSS19.0软件，对各组间数据进行独立样本t检验分析。结果 50 Hz 1 mT PEMF辐照可增强脐带MSC的细胞活性，其中每天辐照4 h组变化最为明显，差异有统计学意义（t=3.505，P<0.05）。PEMF辐照可促进COX2、HGF、IDO和TGF-β等免疫调节因子mRNA的表达，但变化不明显，差异无统计学意义（t=0.982~2.376，均P>0.05），对于干扰素γ（IFN-γ）激活的MSC，PEMF辐照可显著上调COX2、HGF、IDO和TGF-β的mRNA表达水平，差异有统计学意义（t=2.436~3.747，均P<0.05）。50 Hz 1 mT PEMF辐照对脐带MSC的ROS水平、DNA损伤及细胞凋亡率、细胞周期无显著影响。结论 对于IFN-γ激活的MSC，50 Hz 1 mT PEMF可显著上调COX2、HGF、IDO和TGF-β的mRNA表达水平，从而增强脐带MSC的免疫调节能力。50 Hz 1 mT PEMF对脐带MSC的ROS水平、细胞凋亡及DNA损伤无明显影响，此参数下的PEMF辐照对脐带MSC是安全的。
Abstract:
Objective To investigate the effect of 50-Hz, 1-mT pulsed electromagnetic field (PEMF) on the immunomodulation of human umbilical cord mesenchymal stem cells(MSCs) and to evaluate the safety of PEMF as a therapeutic strategy. Methods Changes in cell viability was observed via MTT. Real-time PCR was used to detect the expression levels of cyclooxygenase 2(COX2), indoleamine 2,3-dioxygenase(IDO), IL-10, hepatocyte growth factor(HGF), and transforming growth factor-β(TGF-β) in MSCs. To explore the safety of PEMF, a flow cytometry experiment was conducted to observe apoptosis and reactive oxygen species(ROS) level. DNA damage was detected via comet assay. Data were statistically analyzed via t-test with SPSS 19.0. P<0.05 was considered statistically significant. Results Cell viability increased, with the most obvious increase in 4 h/d(t=3.505, P<0.05). In interferon-γ(INF-γ)-activated MSC, the expression levels of immunomodulation factors improved after PEMF irradiation(t=2.436-3.747, all P<0.05). Furthermore, PEMF did not induce cell apoptosis, ROS generation, and DNA damage. Conclusion 50-Hz, 1-mT PEMF improves MSC immunomodulation and may not cause radiation injury.

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备注/Memo

备注/Memo:
收稿日期:2017-01-09。
基金项目:国家自然科学基金（31670859）；协和青年基金和中央高校基本科研业务费专项资金（3332016100）；中国医学科学院&北京协和医学院中央级公益性科研院所基本科研业务费（2016ZX310198，2016RC310019）；中国医学科学院放射医学研究所创新团队基金（1605）
通讯作者:曹晓沧,Email:doccaoxc@163.com
更新日期/Last Update: 2017-04-24