[1]王月英,吴红英,李德冠,等.不同剂量137Csγ射线照射对小鼠造血系统的影响[J].国际放射医学核医学杂志,2013,37(1):1-4.[doi:10.3760/cma.j.issn.1673-4114.2013.01.001]
　WANG Yue-ying,WU Hong-ying,LI De-guan,et al.Effects of different 137Csγ radiation dose on mouse hematopoietic system[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(1):1-4.[doi:10.3760/cma.j.issn.1673-4114.2013.01.001]
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不同剂量137Csγ射线照射对小鼠造血系统的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第1期
页码:
1-4
栏目:
论著
出版日期:
2013-01-25

文章信息/Info

Title:
Effects of different 137Csγ radiation dose on mouse hematopoietic system
作者:
王月英;  吴红英;  李德冠;  王小春;  宋娜玲;  路璐;  张俊伶;  孟爱民
中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津, 300192
Author(s):
WANG Yue-ying;  WU Hong-ying;  LI De-guan;  WANG Xiao-chun;  SONG Na-ling;  LU Lu;  ZHANG Jun-ling;  MENG Ai-min
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
小鼠; 辐射损伤; 实验性; 造血系统
Keywords:
Mice; Radiation injuries; experimental; Hematopoietic system
DOI:
10.3760/cma.j.issn.1673-4114.2013.01.001
摘要:
目的 探讨不同剂量γ射线照射对不同品系小鼠造血功能的影响。方法 用137Csγ射线分别对IRM-2、ICR、615品系小鼠进行一次性全身4.0 Gyγ射线照射,在照后不同时间检测小鼠外周血白细胞、骨髓有核细胞(BMNC)的变化。对IRM-2小鼠、C57BL/6小鼠进行6.0 Gyγ射线照射,照后第45日,检测小鼠外周血象的变化。结果 IRM-2、ICR、615品系小鼠经4.0 Gy照射后第2日,白细胞和BMNC数量降至最低值。第9日,IRM-2小鼠的BMNC计数与ICR、615小鼠相比,差异有统计学意义(t=3.725,P<0.01;t=8.487,P<0.001).第12日,IRM-2小鼠白细胞计数与ICR、615小鼠相比,差异有统计学意义(t=4.811和4.302,P均<0.001).第21日,IRM-2、ICR、615小鼠白细胞计数分别恢复到正常值的52.0%、60.7%、50.8%;BMNC计数分别恢复到正常值的90.8%、82.1%、75.4%.IRM-2、C57BL/6小鼠经6.0 Gyγ射线照射后第45日,IRM-2小鼠外周血白细胞、红细胞、血红蛋白(Hgb)、红细胞压积(Hct)计数均明显高于C57BL/6小鼠(t=5.629、7.788、4.929和6.064,P均<0.001);IRM-2小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的75.00%、98.95%、98.78%、97.55%;C57BL/6小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的40.60%、93.88%、93.31%、93.84%。结论 IRM-2、ICR、615、C57BL/6小鼠受照后造血功能的恢复趋势相同,但IRM-2小鼠在辐射损伤后其造血功能的恢复较快于ICR、615、C57BL/6小鼠。
Abstract:
Objective To discuss the effects of different dose of radiation on the mouse’s hematopoietic system.Methods Mice of 615 strain,ICR strain and IRM-2 strain were given a one-time 4.0 Gy total body irradiation,and then the changes of peripheral white blood cells and bone marrow nuclear cells (BMNC)among those mice were observed at different time points.Mice of IRM-2 and C57BL/6 were given a one-time 6.0 Gy total body irradiation and the changes of peripheral hematological between the two strains were observed forty-five days after irradiation.Results Two days after irradiation,white blood cell and BMNC counts decreased to the lowest level in all the mice of 615 strain,ICR strain and IRM-2 strain.During the following days,white blood cell and BMNC counts raised again.Nine days after irradiation,BMNC count in IRM-2 mice was significantly higher than that in 615 and ICR mice(t=3.725,P<0.01;t=8.487,P<0.001).Twelve days after irradiation,white blood cell count in IRM-2 mice was significantly higher than that in the 615 and ICR mice (t=4.811 and 4.302,both P<0.001).Twenty-one days after irradiation,compared to the control group,white blood cell count was recovered to 52.0%,60.7% and 50.8% of the normal range in IRM-2,ICR and 615 mice separately and BMNC count was recovered to 90.8%,82.1%,75.4% of the normal range in IRM-2,ICR and 615 mice separately.Forty-five days after 6.0 Gy irradiation,white blood cell,BMNC,hemoglobin and hematocrit counts in IRM-2 mice were significantly higher than those indexes in C57BL/6strain(t=5.629,7.788,4.9289,6.064,all P<0.001).Compared to the control group,white blood cells,red blood cells,hemoglobin and hematocrit were recovered to 75.0%,98.9%,98.7%,and 97.5% of the normal range separately in IRM-2 mice.And white blood cells,red blood cells,hemoglobin and hematocrit were recovered to 40.6%,93.8%,93.3%,and 93.8% of the normal range separately in C57BL/6 mice.Conclusion There are similar recovery trends of hematopoiesis in all strains of mice after irradiation,but IRM-2 mice recovery more quickly than the other strains of mice.

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备注/Memo

备注/Memo:
收稿日期:2012-09-07。
基金项目:国家重点基础研究发展计划(项目编号:2011CB964800-G);国家自然科学基金(项目编号:81072237,81102873);天津市自然科学基金(项目编号:12JCQNJC09100,11JCZDJC19100)
通讯作者:孟爱民,Email:ai_min_meng@126.com
更新日期/Last Update: 1900-01-01