[1]李德冠,路璐,吴红英,等.G-CSF联合SB203580对4Gy照射小鼠免疫系统的作用[J].国际放射医学核医学杂志,2014,38(4):216-218.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
　Li Deguan,Lu Lu,Wu Hongying,et al.The effects of G-CSF combined with SB203580 on the immune system of mice received 4 Gy total body irradiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(4):216-218.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
38
期数:
2014年第4期
页码:
216-218
栏目:
出版日期:
2014-07-25

文章信息/Info

Title:
The effects of G-CSF combined with SB203580 on the immune system of mice received 4 Gy total body irradiation
作者:
李德冠;  路璐;  吴红英;  张俊伶;  孟爱民
中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室, 天津 300192
Author(s):
Li Deguan;  Lu Lu;  Wu Hongying;  Zhang Junling;  Meng Aimin
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China
关键词:
辐射损伤; 粒细胞集落刺激因子; SB203580; 胸腺细胞; 外周血
Keywords:
Radiation injuries; Granulocyte colony-stimulating factor; SB203580; thymocytes; peripheral blood
DOI:
10.3760/cma.j.issn.1673-4114.2014.04.002
摘要:
目的 研究G-CSF联合p38抑制剂SB203580(SB)对免疫细胞辐射损伤的作用。方法 C57BL/6雄性小鼠按体质量随机分为对照组、照射组和给药组。照射组和给药组给予4 Gy全身照射。给药组小鼠给予腹腔注射G-CSF和SB,G-CSF于4 Gy照射后2h和6h给药(1μg/只),每天2次,连续给药5d,SB于照射后24h给药(15 mg/kg),隔日给药,共给药5次。照射后10d测外周血计数,进行白细胞CD4、CD8、B220检测、胸腺细胞CD4、CD8检测和骨髓细胞活性氧检测。结果 照射组外周血计数和CD8、B220比例下降,而胸腺细胞中CD4+CD8+细胞比例及骨髓细胞活性氧水平显著增加。与照射组相比,联合给药组外周血红细胞数、血小板、CD4、CD8细胞比例升高,胸腺细胞中CD4+CD8+细胞比例下降。结论 G-CSF联合SB对4 Gy照射引起的免疫细胞损伤存在保护作用。
Abstract:
Objective To observe the effects of G-CSF combined with SB203580 (SB)on the immune system of mice received 4 Gy total body irradiation (TBI).Methods Thirty male C57BL/6 mice were randomly divided into control group,irradiated group,combined group.The mice in irradiated and combined group received 4 Gy TBI.In combined group,G-CSF (1 μg/each)was intraperitoneally (ip)injected twice one day for 5 days,SB (15 mg/kg)was ip injected every other day after 4 Gy TBI for 5 times.After 4 Gy TBI 10 days,the peripheral bloods were counted,the CD4,CD8,B220 cells in WBC and CD4,CD8 in thymocytes were analyzed by flowcytometry.The reactive oxygen species levels (ROS)of bone marrow cells were detected by microplate reader.Results Compared to the control group,the proportion of CD8,B220 and WBC in the irradiated mice significantly decreased,CD4+CD8+ thymocytes and ROS levels of bone marrow cells increased significantly.Compared to irradiation group,red blood,platelet,CD4 and CD8 cells in peripheral blood,CD4+CD8+ thymocytes decreased in the combined group.Conclusion G-CSF combined with SB has protective effects on the radiation-induced injury in immune system.

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备注/Memo

备注/Memo:
收稿日期:2013-10-29。
基金项目:国家自然科学基金(81129020,81102873,81372928,81072237);天津市自然科学基金(12JCQNJC09100)
通讯作者:孟爱民(Email:ai_min_meng@126.com)
更新日期/Last Update: 1900-01-01