[1]解朋,黄建敏,张芳,等.99Tcm-MAG3-isoDGR-2C分子探针的制备与体内分布的实验研究[J].国际放射医学核医学杂志,2016,40(5):345-349.[doi:10.3760/cma.j.issn.1673-4114.2016.05.004]
 Xie Peng,Huang Jianmin,Zhang Fang,et al.Labeling MAG3-isoDGR-2C with 99Tcm and its biodistribution in mice[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):345-349.[doi:10.3760/cma.j.issn.1673-4114.2016.05.004]
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99Tcm-MAG3-isoDGR-2C分子探针的制备与体内分布的实验研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
40
期数:
2016年第5期
页码:
345-349
栏目:
论著
出版日期:
2016-09-25

文章信息/Info

Title:
Labeling MAG3-isoDGR-2C with 99Tcm and its biodistribution in mice
作者:
解朋 黄建敏 张芳 潘莉萍
050051 石家庄, 河北医科大学第三医院核医学科
Author(s):
Xie Peng Huang Jianmin Zhang Fang Pan Liping
Department of Nuclear Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, China
关键词:
分子探针同位素标记异天冬氨酸-甘氨酸-精氨酸体内分布
Keywords:
Molecular probesIsotope labelingIsoAsp-Gly-ArgBiodistribution
DOI:
10.3760/cma.j.issn.1673-4114.2016.05.004
摘要:
目的 摸索99Tcm-MAG3-isoDGR-2C分子探针的标记条件并研究其在正常昆明小鼠体内的生物学分布。方法 利用葡庚糖酸盐(GH)转换络合法进行标记,即首先用99Tcm标记GH形成99TcmO-GH中间体,再进行99Tcm-MAG3-isoDGR-2C的标记;考察pH值和温度等因素对标记率的影响,采用高效液相色谱法鉴定标记物的放射化学纯度;取30只昆明小鼠随机分成6组,分别于注射99Tcm-MAG3-isoDGR-2C 15、30、60、120、240和360 min后测定其在小鼠体内的分布情况。结果 pH值为4.6、反应温度为100℃时的标记率最高(94.2%)。体内分布研究显示该标记物能够较快地从血液中清除,且心、肺、脾、胃、骨等组织的放射性摄取均很少。结论 99Tcm-MAG3-isoDGR-2C标记率高、血液清除快,具备一定的成为SPECT显像剂的条件。
Abstract:
Objective To analyze the feasibility of labeling MAG3-isoDGR-2C with 99Tcm and investigate the biodistribution of the tracer in normal mice. Methods Glucoheptonate was initially labeled with 99Tcm as the intermediate, followed by MAG3-isoDGR-2C. High-performance liquid chromatography was employed to determine the radiochemical purity. The biodistribution of 99Tcm-MAG3-isoDGR-2C was studied at 15, 30, 60, 120, 240 and 360 min after caudal vein injection in normal mice. Results MAG3-isoDGR-2C was successfully labeled with 99Tcm, and the radiochemical purity was 94.2%. The biodistribution revealed that the tracer was rapidly cleared out from the blood with mild activity in the heart, lung, spleen, stomach, and bone. Conclusion s MAG3-isoDGR-2C was labeled with 99Tcm with high radiochemical purity, and the tracer could be cleared out quickly. Therefore, MAG3-isoDGR-2C is a potential molecular tracer in the study of early cancer diagnosis.

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备注/Memo

备注/Memo:
收稿日期:2016-07-29
基金项目:河北省自然科学基金(H2014206286)
通讯作者:黄建敏,Email:jm_huang2003@126.com
更新日期/Last Update: 1900-01-01