[1]黄少祥,樊体强.塞来昔布对非小细胞肺癌移植瘤的辐射增敏实验研究[J].国际放射医学核医学杂志,2013,37(3):150-152.[doi:10.3760/cma.j.issn.1673-4114.2013.03.006]
　HUANG Shao-xiang,FAN Ti-qiang.Radiosensitization on non-small cell lung cancer induced by celecoxib[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(3):150-152.[doi:10.3760/cma.j.issn.1673-4114.2013.03.006]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第3期
页码:
150-152
栏目:
论著
出版日期:
2013-05-25

文章信息/Info

Title:
Radiosensitization on non-small cell lung cancer induced by celecoxib
作者:
黄少祥1;  樊体强2
1. 300450 天津, 天津市第五中心医院呼吸科;
2. 300192 天津, 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室
Author(s):
HUANG Shao-xiang1;  FAN Ti-qiang2
Department of Respiration, the Fifth Center Hospital of Tianjin, 300450 Tianjin, China
关键词:
癌; 非小细胞肺; 辐射增敏药; 放射疗法; 塞来昔布; 模型; 动物
Keywords:
Carcinoma; non-small-cell lung; Radiation-sensitizing agents; Radiotherapy; Celecoxib
DOI:
10.3760/cma.j.issn.1673-4114.2013.03.006
摘要:
目的 建立裸鼠非小细胞肺癌H460细胞动物模型,观察环氧化酶2选择性抑制剂塞来昔布对H460放疗的增敏作用,并对其作用机理进行初步探讨。方法 将40只荷瘤鼠用完全随机法分成4组,分别为空白对照组、放疗组、塞来昔布组、塞来昔布+放疗组,每组10只。采用灌肠法给予塞来昔布16 mg·kg-1·d-1,给药2h后进行放疗,放疗分割剂量为5 Gy/次,2次/周,4周后处死所有荷瘤鼠,解剖瘤块称重。采用免疫组化方法分析毛细血管扩张性共济失调症突变蛋白(ATM)和表皮生长因子受体(EGFR)表达的变化。结果 空白对照组、塞来昔布组、放疗组、塞来昔布+放疗组肿瘤瘤重分别为(133.62±12.37)、(130.37±12.59)、(81.17±8.29)、(35.51±4.23)mg,塞来昔布+放疗组与放疗组比较差异有统计学意义(t=5.41,P<0.01)。塞来昔布+放疗组的ATM和EGFR表达水平明显降低,与放疗组比较差异有统计学意义(t=4.23和3.17,P均<0.01)。结论 塞来昔布可能通过降低ATM和EGFR的表达水平,增强H460细胞的放疗敏感性,将来可能具有较好的临床应用价值。
Abstract:
Objective To establish the nude mice model of non-small cell lung cancer (NSCLC)H460 cell to investigate the combined effects of radiotherapy and celecoxib.Methods Athymic mice bearing H460 were randomly divided into 4 groups:control,radiotherapy,celecoxib and radiotherapy plus celecoxib group.The administration dose of celecoxib was 16 mg·kg-1·d-1.The mice were treated with radiotherapy 2 hours after administration and the fractionated dose was 5 Gy,2 fractions per week.Mice were killed to detect tumor weight 4 weeks after treatment.The expression levels of ataxia telangiectasis mutated (ATM)and epidermal growth factor receptor (EGFR)in tumor tissues were detected by immune-histochmical method.Results The tumor weight in control,radiotherapy,celecoxib and radiotherapy plus celecoxib group were(133.62±12.37),(130.37±12.59),(81.17±8.29) and (35.51±4.23) mg respectively.There was significant difference between the radiotherapy plus celeeoxib group and the radiotherapy group (t=5.41,P<0.01).The expression levels of ATM and EGFR in the radiotherapy plus celecoxib group were significantly lower than that in radiotherapy group (t=4.23 and 3.17,both P<0.01).Conclusions Celecoxib promotes radiotherapeutic sensitivity of H460 by down-regulating the expression levels of ATM and EGFR.Celecoxib may presents potency in curing human lung cancer.

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备注/Memo

备注/Memo:
收稿日期:2012-12-09。
基金项目:国家自然科学基金(31170804,31240052,31200634)
通讯作者:黄少祥,Email:xuxu991216@yahoo.com.cn
更新日期/Last Update: 1900-01-01