[1]陈凤华,沈秀,李德冠,等.Tat-SmacN7蛋白对食管癌109细胞辐射敏感性的影响[J].国际放射医学核医学杂志,2013,37(3):146-149.[doi:10.3760/cma.j.issn.1673-4114.2013.03.005]
 CHEN Feng-hua,SHEN Xiu,LI De-guan,et al.The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma 109[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(3):146-149.[doi:10.3760/cma.j.issn.1673-4114.2013.03.005]
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Tat-SmacN7蛋白对食管癌109细胞辐射敏感性的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第3期
页码:
146-149
栏目:
论著
出版日期:
2013-05-25

文章信息/Info

Title:
The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma 109
作者:
陈凤华1 沈秀1 李德冠1 付岳1 王津晗2 郭艳婷1 樊飞跃1 刘强1
1. 300192 天津, 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室;
2. 300134 天津, 天津商业大学食品工程学院
Author(s):
CHEN Feng-hua1 SHEN Xiu1 LI De-guan1 FU Yue1 WANG JIN-han2 GUO Yan-ting1 FAN Fei-yue1 LIU Qiang1
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
辐射耐受性凋亡抑制蛋白质类Smac
Keywords:
Radiation toleranceInhibitors of apoptosis proteinsSecond mitochondria-derived activator of caspase
DOI:
10.3760/cma.j.issn.1673-4114.2013.03.005
摘要:
目的 探讨Tat-SmacN7蛋白(其中,Tat:反式激活蛋白;Smac:第二线粒体来源的caspase激活因子;N7:Smac的N端含有Ala-Val-Pro-Ile的七肽)对食管癌109(EC109)细胞放疗敏感性的影响。方法 对EC109细胞给予Tat-SamcN7、照射、以及联合处理,观察不同处理在24 h和48 h时对细胞的抑制作用,采用Western blot检测各组细胞中Smac蛋白表达水平。结果 通过水溶性四唑盐1检测发现Tat-SmacN7单独使用对细胞的抑制作用不明显,但与Tat-SmacN7组和照射组相比,Tat-SmacN7联合放射组可以明显增强对细胞的抑制(24 h:t=16.821和9.825,P<0.05;48 h:t=23.553和11.930,P<0.05)。说明Tat-SmacN7能增强EC109细胞对放射的敏感性。Western blot证明了Tat-SmacN7可以使细胞内Smac蛋白表达增加。结论 Tat-SmacN7蛋白能通过细胞高表达Smac,增强EC109细胞对辐射的敏感性。
Abstract:
Objective To investigate the effects of Tat-SmacN7 protein on sensitivity of esophageal carcinoma 109 (EC109) to radiation.Methods Cells were treated with Tat-SmacN7 peptides,radiation or combination,the inhibition rate of EC109 cells were detected by water-soluble tetrazolium salt-1 assay.The protein level expression of Smac was determined by Western blot.Results The cells were resistant to TatSmacN7 as a single agent,but it could improve the sensitization of EC 109 to radiation.Compared with the TatSamcN7 group and the radiation group,the Tat-SmacN7 combined with radiation group was inhibited significantly (24 h:t=16.821 and 9.825,both P<0.05; 48 h:t=23.553 and 11.930,both P<0.05).Western blot assay showed that Tat-SmacN7 could increase the expression of Smac protein.Conclusions Tat-SmacN7 could improve the radiosensitization of EC 109 cell by increasing the expression of Smac.

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备注/Memo

备注/Memo:
收稿日期:2012-10-26;。
基金项目:国家自然科学基金(31170804);天津市自然科学基金(10JCZDJC16900,11ZCGYSY02400);教育部高等学校博士学科点专项科研基金(20101106110046);卫生行业科研专项(201002009)
通讯作者:刘强,Email:dr.qiangliu@yahoo.com.cn
更新日期/Last Update: 1900-01-01