[1]李敏,孟庆慧,胡旭东,等.B细胞易位基因2的表达水平对肿瘤细胞放射敏感性的影响[J].国际放射医学核医学杂志,2013,37(3):129-134.[doi:10.3760/cma.j.issn.1673-4114.2013.03.001]
　LI Ming,MENG Qing-hui,HU Xu-dong,et al.Effect of B-cell translocation gene 2 alteration on radiosensitivity of cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(3):129-134.[doi:10.3760/cma.j.issn.1673-4114.2013.03.001]
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B细胞易位基因2的表达水平对肿瘤细胞放射敏感性的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第3期
页码:
129-134
栏目:
论著
出版日期:
2013-05-25

文章信息/Info

Title:
Effect of B-cell translocation gene 2 alteration on radiosensitivity of cancer cells
作者:
李敏1;  孟庆慧1;  胡旭东1; 2;  焦旸1;  徐加英1;  樊赛军3
1. 215123 苏州, 苏州大学医学部放射医学与公共卫生学院肿瘤放射生物学实验室;
2. 250117 济南, 山东省肿瘤医院放疗科;
3. 300192 天津, 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室
Author(s):
LI Ming1;  MENG Qing-hui1;  HU Xu-dong1; 2;  JIAO Yang1;  XU Jia-ying1;  FAN Sai-jun3
Lab oratory of Tumor Radiobiology, School of Radiation and Public Health, Medical College of Soochow University, Jiangsu 215123, China
关键词:
肿瘤; 辐射耐受性; B细胞易位基因2; 基因; BRCA1
Keywords:
Neoplasms; Radiation tolerance; B-cell translocation gene 2; Gene; BRCA1
DOI:
10.3760/cma.j.issn.1673-4114.2013.03.001
摘要:
目的 研究B细胞易位基因2(BTG2)表达水平的改变对肿瘤细胞放射敏感性的影响。方法 通过pcDNA3-BTG2脂质体转染的方法提高细胞的BTG2的表达水平,利用噻唑蓝和细胞克隆形成实验研究细胞放射敏感性的改变,应用Wester blot方法研究蛋白表达水平的变化。结果 噻唑蓝和细胞克隆形成实验结果显示,在不同剂量的γ射线照射后,提高BTG2的表达水平可明显提高乳腺癌MCF-7和MDA-MB-231细胞的放射敏感性。免疫共沉淀-Wester blot实验结果显示BTG2蛋白与DNA损伤修复和抗氧化蛋白乳腺癌易感基因1(BRCA1)相互作用,形成复合物。高表达的BRCA1明显地抑制了BTG2高表达对乳腺癌细胞放射敏感性的调节作用,而降低BRCA1的表达水平则提高了BTG2对乳腺癌细胞放射敏感性的调节作用。另外,肺癌细胞放射敏感性与其所含的BRCA1的表达水平成反比,而与BTG2的表达水平成正比。结论 BTG2的高表达明显提高了肿瘤细胞的放射敏感性,其机制可能与其同BRCA1形成复合物有关。
Abstract:
Objective To investigate the effects of B-cell translocation gene 2 (BTG2) overexpression on the radiosensitivity of cancer cells.Methods Cancer cells with overexpression of BTG2 were established via stable transfection of full-length human BTG2 cDNA which was inserted into a mammalian expression plasmid pcDNA3 (pcDNA3-BTG2).Cell survival was determined by thiazolyl blue tetrazolium bromide (MTT) and clonogenic survival assays.Protein-protein interaction was performed by immune precipitation (IP)-Western blot assay.Protein expression was assayed by Western blot assay.Results As demonstrated in MTT assay and clonogenic survival assay,enforced expression of BTG2 significantly enhanced radiosenstivity of human breast cancer MCF-7 and MADMB-231 cells.The BTG2 protein was able to be determined in the breast cancer susceptibility genel (BRCA1) IP.Silence of BRCA1 enhanced the increased radiosensitivity by BTG2,however,co-over-expression of BRCA1 reduced the BTG2-mediated radiosenstivity.Finally,the radiosensitivity of lung cancer cell lines tested exhibited a positive relationship with the levels of BTG2 protein expression and a negative correlation with the levels of BRCA1 protein expression.Conclusion The present study further demonstrates that there is a significant relationship of radiosenstivity with BTG2 and BRCA1 expression,suggesting that BTG2 may be a new and important target in cancer radiotherapy via its binding to BRCA1.

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备注/Memo

备注/Memo:
收稿日期:2013-02-17。
基金项目:国家自然科学基金(81172127,81071906)
通讯作者:樊赛军,Email:fansaijun@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01