[1]马铁军,王毅,安源,等.多色荧光原位杂交显带技术在高、低传能线密度辐射损伤中的应用[J].国际放射医学核医学杂志,2012,36(3):168-171.[doi:10.3760/cma.j.issn.1673-4114.2012.03.012]
 MA Tie-jun,WANG Yi,AN Yuan,et al.Multicolour-banding fluorescence in situ hybridisation analysis of radiation damage induced by high- and low-linear energy transfer rays[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(3):168-171.[doi:10.3760/cma.j.issn.1673-4114.2012.03.012]
点击复制

多色荧光原位杂交显带技术在高、低传能线密度辐射损伤中的应用(/HTML)
分享到:

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第3期
页码:
168-171
栏目:
辐射剂量、损伤与防护
出版日期:
2012-05-25

文章信息/Info

Title:
Multicolour-banding fluorescence in situ hybridisation analysis of radiation damage induced by high- and low-linear energy transfer rays
作者:
马铁军 王毅 安源 王东平 秦雪琴 范青萍 张娟
解放军第五三七医院防护科, 宝鸡 721006
Author(s):
MA Tie-jun WANG Yi AN Yuan WANG Dong-ping QING Xue-qin FAN Qing-ping ZHANG Juan
Department of Pratection, the 537 th Hospital of People’s Liberation Army, Baoji 721006, China
关键词:
染色体畸变辐射损伤传能线密度荧光原位杂交显带多色
Keywords:
Chromosome aberrationsRadiation injuriesLinear energy transferFluorescence in situ hybridization multicolour-banding
DOI:
10.3760/cma.j.issn.1673-4114.2012.03.012
摘要:
淋巴细胞染色体畸变率与电离辐射密切相关,并且已经广泛应用于辐射损伤的生物剂量评估。多色荧光原位杂交显带技术(mBAND)是染色体畸变检测的重要新兴技术,并且逐步应用于不同传能线密度(LET)射线所引起的染色体畸变检测。该文对mBAND技术在X、γ射线所属的低LET射线和太空射线及高能重离子流所属的高LET射线中的应用,二者引起不同染色体畸变类型以及产生的不同辐射生物剂量效能作综合阐述。
Abstract:
Lymphocyte chromosome aberrations rate is closely related with ionizing radiation, and it has been widely used in biological dose evaluating. Multicolour-banding fluorescence in situ hybridisation(mBAND) is a newly-emerging technology in chromosome aberrations checking. It has been used in chromosome aberrations checking of different linear energy transfer(LET) rays. This article briefly reviewed mBAND using in different chromosome aberrations checking and different radiation biological evaluation by both low LET(such as X and γ rays) and high LET(such as space lines and energetic heavy ions).

参考文献/References:

[1] Chudoba I,Hickmann G,Friedrich T,et ah mBAND:a high reso-lution muhicolor banding technique for the detection of complex intrachromosomal aberrations.Cytogenet Genome Res,2004,104(1-4):390-393.
[2] ]Nikjoo H,Uehara S,Wilson WE,et al.Track structure in radiation biology:theory and applications.Int J Radiat Biol,1998,73(4):355-364.
[3] Cucinotta FA,Nikjoo H,Goodhead DT.Model for radial depen-dence of frequency distributions for energy imparted in nanometer volumes from HZE particles.Radiat Res,2000,153(4):459-468.
[4] Lobrich M,Cooper PK,Rydberg B.Non-random distribution of DNA double-strand breaks induced by particle irradiation.Int J Radiat Biol,1996,70(5):493-503.
[5] Hada M,Georgakilas AG.Formation of clustered DNA damage after high-LET irradiation:a review.J Radiat Res,2008,49(3):203-210.
[6] ForayN,Arlett CF,Malaise EP.Radiation-induced DNA double-strand breaks and the radiosensitivity of human cells:a closer look.Biochimie,1997,79(9-10):567-575.
[7] Pinto M,Prise KM,Michael BD,et al.Quantification of radiation induced DNA double-strand breaks in human fibroblasts by PFGE:testing the applicability of random breakage models.Int J Radiat Biol,2002,78(5):375-388.
[8] Johnson KL,Nath J,Pluth JM,et al.The distribution of chromo-some damage,non-reciprocal translocations and colonal aberrations in lymphocytes from Chernobyl clear-up workers.Mutat Res,1999,439(1):72-85.
[9] Chudoba I,Plesch A,Lrch T,et al.High resolution muhicolor-banding:a new technique for refined FISH analysis of human chro-mosomes.Cytogenet Cell Genet,1999,84(3-4):156-160.
[10] Johannes C,Chudoba I,Obe G.Analysis of X-ray-induced aberra-tions inhuman chromosome 5 using high-resolution muhicolor banding FISH (mBAND).Chromosome Res,1999,7(8):625-633.
[11] Hande MP,Azizova TV,Geard CR,et al.Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome.Am J Hum Genet,2003,72(5):1162-1170.
[12] Tawn EJ,Whitehouse CA,Holdsworth D,et al.mBAND analysis of chromosome aberrations in lymphocytes exposed in vitro to alpha-particles and gamma-rays.Int J Radiat Biol,2008,84(6):447-453.
[13] Hada M,Cucinotta FA,Gonda SR,et al.mBAND analysis of chro-mosomal aberrations in human epithelial cells exposed to low-and high-LET radiation.Radiat Res,2007,168(1):98-105.
[14] Horstmann M,Durante M,Johannes C,et al.Space radiation does not induce a significant increase of intrachromosomal exchanges in astronauts’ lymphocytes.Radiat Environ Biophys,2005,44(3):219-224.
[15] Johannes C,Horstmann M,Durante M,et al.Chromosome intra-changes and interchanges detected by multicolor banding in lym-phocytes:searching for clastogen signatures in human genome.Ra-diat Res,2004,161(5):540-548.
[16] Pignalosa D,Ritter S,Durante M.Inversion in chromosome 10 of human thyroid cells induced by accelerated heavy ions.Radiat Res,2010,174(1):14-19.
[17] Ponomarev AL,Cucinotta FA.Chromatin loops are responsible for higher counts of small DNA fragments induced by high-LET radia-tion,while chromosomal domains do not affect the fragment sizes.Int J Radiat Biol,2006,82(4):293-305.
[18] Lee R,Sommer S,Hartel C,et al.Intra-and inter-chromosome aber-rations in human lymphocytes exposed to radiation in vivo and in vitro detected by mBAND.GSI Scientific Report 2008,2009:373.
[19] Horstmann M,Durante M,Obe G.Distribution of breakpoints and fragment sizes in human chromosome 5 after heavy-ion bombard-ment.Int J Radiat Biol,2004,80(6):437-443.
[20] Hada M,Gersey B,Saganti PB,et al.mBAND analysis of chromo-some aberrations in human epithelial cells induced by gamma-rays and secondary neutrons of low dose rate.Mutat Res,2010,701(1):67-74.
[21] Hada M,Zhang Y,Feiveson A,et al.Association of inter and intra-chromosomal exchanges with the distribution of low-and high-LET radiation-induced breaks in chromosomes.Radiat Res,2011,176(1):25-37.
[22] Gijsbers AC,Schoumans J,Ruivenkamp CA.Interpretation of array comparative genome hybridization data:a major challenge.Cyto-genet Genome Res,2011,135(3-4):222-227.

相似文献/References:

[1]包明月,刘玉龙.应用间充质干细胞治疗急性辐射损伤的研究现状与进展[J].国际放射医学核医学杂志,2016,40(1):65.[doi:10.3760/cma.j.issn.1673-4114.2016.01.013]
 Bao Mingyue,Liu Yulong.Recent advances in the application of mesenchymal stem cells for treatment of acute radiation injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(3):65.[doi:10.3760/cma.j.issn.1673-4114.2016.01.013]
[2]贺欣,储小飞,罗丹,等.穿心莲药物对辐射损伤效应影响的初步研究[J].国际放射医学核医学杂志,2015,39(5):375.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 005]
 he xin,chu xiaofei,luo dan,et al.exploration of andrographolide effects on radiation injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):375.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 005]
[3]路璐,张俊伶,李德冠,等.6 gy 137cs γ射线照射对小鼠造血功能损伤的动态观察研究[J].国际放射医学核医学杂志,2015,39(5):393.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 009]
 lu lu*,zhang junling,li deguan,et al.effects of 6 gy 137cs γ-irradiattion on the hematopoietic system of mice[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):393.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 009]
[4]刘惠惠,李险峰.放射性心脏损伤诊断和防治的临床研究进展[J].国际放射医学核医学杂志,2016,40(5):374.[doi:10.3760/cma.j.issn.1673-4114.2016.05.008]
 Liu Huihui,Li Xianfeng.Clinical research progress in diagnosis,prevention and treatment of radiation-induced heart disease[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(3):374.[doi:10.3760/cma.j.issn.1673-4114.2016.05.008]
[5]张倩如,李海涛,田红旗.小分子辐射防护药物的研究进展[J].国际放射医学核医学杂志,2016,40(5):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
 Zhang Qianru,Li Haitao,Tian Hongqi.Small molecule compounds against radiation:research advances[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(3):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
[6]王浩,沈秀,靳瑾,等.“四物汤”对辐射危害的辅助保护作用研究[J].国际放射医学核医学杂志,2015,39(2):121.[doi:10.3760/cma.j.issn.1673-4114.2015.02.004]
 Wang Hao,Shen Xiu,Jin Jin,et al.Protective activity of Siwu Decoction against radiation hazards[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):121.[doi:10.3760/cma.j.issn.1673-4114.2015.02.004]
[7]曹婉婷,李浣洋,陈雪英,等.正常组织在放射治疗中并发的迟发性损伤[J].国际放射医学核医学杂志,2015,39(3):260.[doi:10.3760/cma.j.issn.1673-4114.2015.03.016]
 Cao Wanting,Li Huanyang,Chen Xueying,et al.Late effects of radiotherapy on normal tissue[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):260.[doi:10.3760/cma.j.issn.1673-4114.2015.03.016]
[8]陈雪梅,周树云,范源,等.表没食子儿茶素没食子酸酯对131I辐射损伤所致甲减大鼠模型抗氧化体系的保护作用[J].国际放射医学核医学杂志,2015,39(4):311.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
 Chen Xuemei,Zhou Shuyun,Fan Yuan,et al.Protection of antioxidant system of EGCG on the thyroid in rat model from 131I radiation damage[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(3):311.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
[9]李德冠,路璐,吴红英,等.G-CSF联合SB203580对4Gy照射小鼠免疫系统的作用[J].国际放射医学核医学杂志,2014,38(4):216.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
 Li Deguan,Lu Lu,Wu Hongying,et al.The effects of G-CSF combined with SB203580 on the immune system of mice received 4 Gy total body irradiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(3):216.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
[10]张洪明,李险峰.放射性心脏损伤动物模型的研究进展[J].国际放射医学核医学杂志,2014,38(6):412.[doi:10.3760/cma.j.issn.1673-4114.2014.06.015]
 Zhang Hong-ming,Li Xian-feng.Progress in animal model of radiation-induced heart disease[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(3):412.[doi:10.3760/cma.j.issn.1673-4114.2014.06.015]

备注/Memo

备注/Memo:
收稿日期:2012-02-05。
通讯作者:马铁军(Email:matiejun19860926@yahoo.com.cn)
更新日期/Last Update: 1900-01-01