[1]李德冠,唐卫生,牟感恩,等.5-甲氧基色胺-α-硫辛酸盐对6.0 Gy受照小鼠造血系统的辐射防护作用[J].国际放射医学核医学杂志,2017,41(1):19-22,32.[doi:10.3760/cma.j.issn.1673-4114.2017.01.004]
　Li Deguan,Tang Weisheng,Mu Ganen,et al.Protective effects of 5-methoxytryptamine-α-lipoic acid salt on mice exposed to 6.0 Gy total body irradiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2017,41(1):19-22,32.[doi:10.3760/cma.j.issn.1673-4114.2017.01.004]
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5-甲氧基色胺-α-硫辛酸盐对6.0 Gy受照小鼠造血系统的辐射防护作用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
41
期数:
2017年第1期
页码:
19-22,32
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Protective effects of 5-methoxytryptamine-α-lipoic acid salt on mice exposed to 6.0 Gy total body irradiation
作者:
李德冠;  唐卫生;  牟感恩;  姜道利;  田真源;  孙召金;  张曼;  周则卫
300192 天津, 中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室
Author(s):
Li Deguan;  Tang Weisheng;  Mu Gan’en;  Jiang Daoli;  Tian Zhenyuan;  Sun Zhaojin;  Zhang Man;  Zhou Zewei
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300192, China
关键词:
辐射防护; 辐射损伤; 活性氧; NADPH氧化酶4; 5-甲氧基色胺-α-硫辛酸盐
Keywords:
Radiation protection; Radiation injuries; Reactive oxygen species; NADPH oxidase 4; 5-methoxytryptamine-α-lipoic acid salt
DOI:
10.3760/cma.j.issn.1673-4114.2017.01.004
摘要:
目的 探讨新化合物5-甲氧基色胺-α-硫辛酸盐（MLA）对亚致死剂量受照小鼠造血系统损伤的辐射防护作用。方法 将15只C57BL/6小鼠完全随机分为对照组、照射组和照射+MLA组。对照组接受假照射（0 Gy），其余两组进行6.0 Gy全身137Cs γ射线照射。照射后2 h将照射+MLA组小鼠按10 mg/kg灌胃给药，持续给药3 d。待照射后30 d处死小鼠，取外周血和单侧骨髓细胞进行计数，检测骨髓细胞克隆形成能力、造血细胞活性氧以及烟酰胺腺嘌呤二核苷磷酸氧化酶4（NOX4）的表达。结果 与对照组比较，照射组小鼠骨髓有核细胞计数、粒细胞-单核细胞集落生成数量（CFU-GM）均明显下降（t=9.304、7.493,均P<0.05）；骨髓细胞活性氧水平和NOX4表达显著升高（t=14.74、53.12,均P<0.05），且差异有统计学意义。与照射组相比，照射+ＭＬＡ组小鼠外周血WBC、CFU-GM显著升高（t=4.858、3.947,均P<0.05）；骨髓细胞活性氧水平和NOX4表达显著下降（t=11.21、33.93,均P<0.05），且差异有统计学意义。结论 ＭＬＡ对辐射引起的造血系统损伤有一定的防护作用。
Abstract:
Objective To study the protective effects of 5-methoxytryptamine-α-lipoic acid salt(MLA), a new synthetized compound, on the hematopoietic system of mice exposed to sublethal dose of total body irradiation(TBI). Methods Fifteen C57BL/6 mice were randomly assigned into three groups:control, irradiation, and MLA+irradiation. The mice in the control group received sham TBI, whereas the mice in the other groups underwent exposure to 6.0 Gy 137Cs γ-ray TBI. The mice in the MLA group were first administered with 10 mg/kg MLA after exposure to 6.0 Gy TBI for 2 h. The MLA was administered successively for 3 d. The peripheral blood and bone marrow nucleated cells(BMNCs) were then counted after the mice were exposed to TBI for 30 d. The CFU-GM ability, reactive oxygen species(ROS) levels, and nicotinamide adenine dinucleotide phosphate oxidase 4(NOX4) expression in the hematopoietic cells were also detected. Results In contrast to those of the control group, the number of BMNCs and the number CFU-GM in the irradiated groups decreased significantly(t=9.304, 7.493, both P<0.05), and the ROS levels and the NOX4 expression in the bone marrow cells of the irradiated groups increased(t=14.74, 53.12, both P<0.05). Compared with those of the irradiation group, the number of white blood cells and the number of BMNCs were elevated by MLA(t=4.858, 3.947, both P<0.05). The ROS levels and NOX4 expression in the bone marrow cells were inhibited by MLA(t=11.21, 33.93, both P<0.05). Conclusion MLA can protect mice from hematopoietic injury induced by irradiation.

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备注/Memo

备注/Memo:
收稿日期:2016-06-29。
基金项目:国家自然科学基金（81372928，81573094）；天津市自然科学基金重点项目（15JCZDJC35200，15JCZDJC33400）；中国医学科学院放射医学研究所所基金（1526）
通讯作者:周则卫,Email:zhouzewei@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01