[1]徐畅,王彦,杜利清,等.shRNA干扰沉默Net1基因对电离辐射损伤反应的影响[J].国际放射医学核医学杂志,2013,37(3):135-138.[doi:10.3760/cma.j.issn.1673-4114.2013.03.002]
　XU Chang,WANG Yan,DU Li-qing,et al.The effects of short hairpin RNA-mediated silencing Net1 on ionizing radiation-induced damage responses[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(3):135-138.[doi:10.3760/cma.j.issn.1673-4114.2013.03.002]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第3期
页码:
135-138
栏目:
论著
出版日期:
2013-05-25

文章信息/Info

Title:
The effects of short hairpin RNA-mediated silencing Net1 on ionizing radiation-induced damage responses
作者:
徐畅;  王彦;  杜利清;  曹嘉;  刘强
300192 天津, 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室
Author(s):
XU Chang;  WANG Yan;  DU Li-qing;  CAO Jia;  LIU Qiang
Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China
关键词:
电离辐射; RNA干扰; 细胞凋亡; 神经上皮细胞转化基因1
Keywords:
Ionizing radiation; RNA interference; Apoptosis; Neuroepithelioma transforming gene
DOI:
10.3760/cma.j.issn.1673-4114.2013.03.002
摘要:
目的 研究神经上皮细胞转化基因1(NET1)在电离辐射损伤反应中的生物学功能。方法 运用RNA干扰技术,在细胞中特异性沉默Net1基因,然后通过集落存活实验和免疫印迹法研究抑制Net1表达对细胞的电离辐射损伤反应的影响。结果 Net1敲低表达的细胞对电离辐射的敏感性增强,表现为更多细胞发生了凋亡,并且在γ射线照射后,毛细血管扩张性共济失调症突变蛋白(ATM)和细胞周期检查点激酶2(Chk2)的磷酸化水平也比对照细胞增强。结论 Net1对于受到电离辐射的细胞有一定的保护作用,很可能在电离辐射损伤修复中具有重要作用。
Abstract:
Objective To study the biological roles of the neuroepithelioma transforming gene 1 (Netl) in the cellular responses to ionizing radiation(IR)-induced damage.Methods Specific shRNA was used to deplete Netl in cells.The effects of Netl depletion on the cellular responses to ionizing radiation were investigated through the colonogenic survival assay and immunoblotting analysis of DNA damage response proteins’ phosphorylation.Results Netl-depleted cells were more sensitive to IR,with a significantly increased induction of apoptosis.In response to IR,the phosphorylation levels of ataxiatelangiectasia mutated and checkpoint kinase 2 were much higher in the Net1-depleted cells than that in the control cells.Conclusion Net1 protects IR-treated cells from apoptosis and possibly plays an important role in IR-induced damage response and repair.

参考文献/References:

[1] Wennerberg K,Der CJ. Rho-family GTPases:it’s not only Rac and Rho(and I like it). J Cell Sci,2004,117(Pt 8):1301-1312.
[2] Rossman KL,Der CJ,Sondek J. GEF means go:turning on RHO GTPases with guanine nucleotide-exchange factors. Nat Rev Mol Cell Biol,2005,6(2):167-180.
[3] Schmidt A,Hall A. The Rho exchange factor Netl is regulated by nuclear sequestration. J Biol Chem,2002,211(17):14581-14588.
[4] Guerra L,Carr HS,Richter-Dahlfors A,et al. A bacterial cytotoxin identifies the RhoA exchange factor Netl as a key effector in the response to DNA damage. PLoS One,2008,3(5):e2254.
[5] Dubash AD,Guilluy C,Srougi MC,et al. The small GTPase RhoA localizes to the nucleus and is activated by Netl and DNA damage signals. PLoS One,2011,6(2):el7380.
[6] Matsuoka S,Rotman G,Ogawa A,et al. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. Proc Natl Acad Sci USA,2000,97(19):10389-10394.
[7] Frisan T,Cortes-Bratti X,Chaves-Olarte E,et al. The Haemophilus ducreyi cytolethal distending toxin induces DNA double-strand breaks and promotes ATM-dependent activation of RhoA. Cell Microbiol,2003,5(10):695-707.

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备注/Memo

备注/Memo:
收稿日期:2013-04-15。
基金项目:国家自然科学基金(31200634);天津市回国人员绿色通道项目(12JCYBJC32900);中国医学科学院放射医学研究所引进人才启动基金(所人1232);协和青年基金和中央高校基本科研业务费专项资金(2012J05,2012G01)
通讯作者:刘强,Email:liuqiang@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01