[1]王璐,李晴,徐畅,等.sirna干扰sirt1对辐射后il-6表达的影响[J].国际放射医学核医学杂志,2015,39(5):423-426.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
　wang lu,li Qing,xu chang,et al.effect of sirt1 sirna interference on the expression of il-6 after radiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):423-426.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
39
期数:
2015年第5期
页码:
423-426
栏目:
论著
出版日期:
2015-09-15

文章信息/Info

Title:
effect of sirt1 sirna interference on the expression of il-6 after radiation
作者:
王璐; 李晴; 徐畅; 王彦; 杜利清; 王芹; 杨福军; 孙元明; 刘强
300192 天津，中国医学科学院北京协和医学院放射医学研究所，天津市放射医学与分子核医学重点实验室
Author(s):
wang lu;  li Qing;  xu chang;  wang yan;  du liqing;  wang Qin;  yang fujun;  sun yuanming;  liu Qiang.
tianjin key laboratory of radiation medicine and molecular nuclear medicine,　institute of radiation medicine chinese academy of medical sciences,　tianjin 300192,　china
关键词:
沉默信息调节蛋白质类; rna干扰; 间充质干细胞; 白细胞介素6; 辐射; 电离
Keywords:
silent information regulator proteins;  rna interference;  mesenchyal stem cells;  interleukin- 6;  ironing;  radiation
DOI:
10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 017
摘要:
目的 研究sirna干扰沉默信息调节因子2相关酶1（sirt1）对间充质干细胞（mscs）电离辐射后诱导的炎性因子白细胞介素6（il-6）的影响，探究sirt1的辐射防护作用。方法 使用 0、2、4、8 gy照射mscs，分别在照后3、6、12、24 h提取总rna和总蛋白，检测il-6表达水平；将mscs分为空白对照组、单纯照射组和sirt1干扰联合照射组，使用western blot、rt-pcr检测sirt1和il-6胞内表达。结果 mscs在电离照射后，il-6的表达水平先升高后降低；在照后12 h达到最高，24 h恢复基准水平；而联合sirt1干扰会引起il-6水平进一步升高，加重mscs电离辐射后的炎症反应。结论 sirt1可能通过抑制电离辐射诱导的炎性因子il-6的表达，发挥辐射防护作用。
Abstract:
objective to investigate the effect of the knockdown of ilent information regulation factor 2 related enzyme on inflammatory factor il-6 produced in irradiated mesenchymal stem cells（mscs） and explore the radioprotective effect of sirt1. methods mscs was treated with different dose of irradiation, and the expression of il-6 in different time was detected by western-blot and rt-pcr；mscs were divided into control group, radiation group and combination group，the expression of il-6 and sirt1 was detected by western-blot and rt-pcr. results ionizing radiation could lead to the increasing of il-6 expression in mscs. in detail，the expression level of il-6 protein reached the peak in 12 hours after radiation and then slowly recovered to the baseline in 24 hours after radiation. and interfered with sirt1 could aggravate the expression of ionizing radiation-induced il-6 and worsen inflammation. conclusion sirt1 may inhibit the ionizing radiation-induced expression of inflammatory cytokines il-6.

参考文献/References:

[1]price nl, gomes ap, ling aj, et al. sirt1 is required for ampk activation and the beneficial effects of resveratrol on mitochondrial function[j]. cell metab, 2012, 15（5）：675-690.
[2]fu ye, wang y, du lQ, et al. resveratrol inhibits ionising irradiation-induced inflammation in mscs by activating sirt1 and limiting nlrp-3 inflammasome activation[j]. int j mol sci, 2013, 14（7）：14105-14118.
[3]chamberlain g, fox j, ashton b, et al. concise review：mesenchymal stem cells：their phenotype, differentiation capacity, immunological features, and potential for homing[j]. stem cells, 2007, 25（11）：2739-2749.
[4]scheller j, chalaris a, schmidt-arras d, et al. the pro-and anti-inflammatory properties of the cytokine interleukin-6[j]. biochim biophys acta, 2011, 1813（5）：878-888.
[5]calabrese lh, rose-john s. il-6 biology：implications for clinical targeting in rheumatic disease[j]. nat rev rheumatol, 2014, 10（12）：720-727.
[6]taniguchi k, karin m. il-6 and related cytokines as the critical lynchpins between inflammation and cancer[j]. semin immunol, 2014, 26（1）：54-74.
[7]王璐, 徐畅, 刘强. 沉默信息调节因子2相关酶1在炎症反应反应中的调节机制研究进展[j]. 国际免疫学杂志, 2015, 38（3）：252-255．
[8]liu tf, yoza bk, el gazzar ma, et al. nad（+） -dependent sirt1 deacetylase participates in epigenetic reprogramming during endotoxin tolerance[j]. j biol chem, 2011, 286（11）：9856-9864.
[9]kauppinen a, suuronen t, ojala j, et al. antagonistic crosstalk between nf-κb and sirt1 in the regulation of inflammation and metabolic disorders[j]. cell signal, 2013, 25（10）：1939-1948.
[10]韩昀, 鲁建华, 张文芳, 等. 白藜芦醇对兔急性高眼压视网膜节细胞nf-κb表达和il-6浓度的影响[j]． 国际眼科杂志, 2011, 11（6）：958-960.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（31300695, 31170804）；天津市自然科学基金（13jcybjc23500,13jcQnjc11600）；中国医学科学院放射医学研究所发展基金（1553）
通信作者：刘强（email：liuqiang@irm-cams.ac.cn）
更新日期/Last Update: 2015-09-15