[1]杨爱民,于燕,邓惠兴,等.99Tcm-HL91和18F-FDG显像与放疗关系的实验研究[J].国际放射医学核医学杂志,2011,35(4):217-219,248.[doi:10.3760/cma.j.issn.1673-4114.2011.04.005]
　YANG Ai-min,YU Yan,DENG Hui-xing,et al.Experimental study on the relationship between radiotherapy effect and 99Tcm-HL91 and 18F-FDG imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(4):217-219,248.[doi:10.3760/cma.j.issn.1673-4114.2011.04.005]
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99Tcm-HL91和18F-FDG显像与放疗关系的实验研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
35
期数:
2011年第4期
页码:
217-219,248
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Experimental study on the relationship between radiotherapy effect and 99Tcm-HL91 and 18F-FDG imaging
作者:
杨爱民1;  于燕2;  邓惠兴2;  李杰2
1. 西安交通大学医学院第一附属医院核医学科, 西安 710061;
2. 西安交通大学医学院公共卫生系, 西安 710061
Author(s):
YANG Ai-min1;  YU Yan2;  DENG Hui-xing2;  LI Jie2
Department of Nuclear Medicine, the First Hospital, Medical School of Xi’an Jiaotong University, Xi’an, 710061, China
关键词:
放射疗法; 氟脱氧葡萄糖F18; 体层摄影术; 发射型计算机; 单光子; HL-91
Keywords:
Radiotherapy; Fluorodeoxyglucose F18; Tomography; emission-computed; single-photon; HL91
DOI:
10.3760/cma.j.issn.1673-4114.2011.04.005
摘要:
目的 用99Tcm-4,9-二氮-2,3,10,10-四甲基十二烷-2,11-二酮肟(99Tcm-HL91)和18F-FDG为显像剂,探讨肿瘤放疗前、后的乏氧状态与放疗疗效之间的关系。方法 ①动物:选取20只昆明种、清洁级健康成年雄性小鼠供实验用(另备5只用于腹水模型),采用完全随机方法分为2组(对照组和实验组),每组10只。②细胞:将S180腹水瘤细胞株复苏后分别接种于5只小鼠腹腔,待腹水形成后,抽取1 ml稀释至活瘤细胞计数大于2×106个/ml瘤细胞悬液,于每只小鼠右后肢皮下接种0.2 ml。待瘤体长至1~1.5 cm时用于实验。③99Tcm-HL91显像:通过小鼠尾静脉注射99Tcm-HL9137 MBq,4h后分别于放疗前、放疗后1h、2d和10d进行最像。④18F-FDG显像:分别于放疗前和放疗后11d进行18F-FDG显像。显像前12 h禁食,小鼠尾静脉注射18F-FDG 11.5MBq,30 min后腹腔注射0.1%戊巴比妥钠麻醉。⑤放射治疗:第1次99Tcm-HL91和18F-FDG显像结束后,2组小鼠分别给予0 Gy和8 Gy的X线照射。⑥图像处理和半定量分析:于图像上肿瘤部位和小鼠肺野设等大感兴趣区(ROI),获得显像中瘤/非瘤(T/NT)摄取比值(UR)。结果 ①放疗前和放疗后1h、2d、10d 99Tcm-HL91的UR分别为3.53±1.62、3.41±1.42、2.55±1.57和1.26±0.03,表明放疗后肿瘤出现再氧合,乏氧状态依次减低。对照组各时相的UR值分别为3.62±1.65、3.02±1.94、4.10±1.48和2.96±2.02,UR无明显递减趋势,表明肿瘤乏氧状态未见减低,甚至在2d时段出现加重。②放疗前和放疗后11 d 18F-FDG显像的UR分别为2.49±1.29和1.49±0.56,UR亦呈现依次递减趋势,与99Tcm-HL91显像一致。表明放疗后肿瘤葡萄糖代谢活性减低。对照组的UR分别为2.22±0.45和1.89±0.08,UR也出现减低,但变化不明显,而且大部分小鼠在显像前死亡。结论 放疗前后99Tcm-HL91显像的UR能够较好地评价肿瘤乏氧水平,并可初步评价疗效,为在活体评价放疗后肿瘤再氧合过程提供了一种新方法。
Abstract:
Objective To investigate the relationship between radiotherapy effect and 99Tcm-4, 9-diaza-2, 3, 10, 10-tetramethyldodecan-2, 11-dioxime(99Tcm-HL91) and 18F-FDG imaging in S180 mouse. Methods ① Animals:twenty male Kunming mice were randomly divided into two groups of radiotherapy and non-radiotherapy control group. ②Cells:S180 cell lines were injected into peritoneal cavity of the other 5 mice. When the SI 80 tumor developed, 1 ml liquid were dripped and diluted to the suspension solution of 2×106cells. Then, 0.2 ml cells solution were was injected into the hippo of right rear leg of mice. The mousemodel was used to experiment while the tumor dimension developed to 1-1.5 cm. ③99Tcm-HL91 imaging:37 MBq 99Tcm-HL91 was injected into mouse models by tail vein. Alter 4h, SPECT were performed before and at 1h, 2d and 10d after radiotherapy. ④18F-FDG imaging:11.5 MBq 18F-FDG was injected into mouse models by tail vein. After 30 min, SPECT were perfored before and after radiotherapy at the time of 11d. ⑤Radiotherapy:two groups of mice were irradiated to 0 Gy and 8 Gy X-ray after the first 99Tcm-HL91 and 18F-FDG imaging. ⑥Images analysis:the region of interest(ROI) region, in tumor and lung site, was drawed to calculate the uptake ratio(UR). Results Before and at 1h, 2d, and 10d after radiotherapy, the UR in99Tcm-HL91 imaging were 3, 53±1.62, 3.41 ±1.42, 2.55±1.57 and 1.26±0.03, respectively, while the UR were 3.62 ±1.65, 3.02±1.94, 4.10±1.48 and 2.96±2.02 in control group. This revealed that tumors hypoxic level was decreased after radiation and suggested that tumors developed reoxygenation. Before and at lid after radiotherapy, the UR in 18F-FDG imaging were 2.49±1.29 and 1.49±0.56, while the UR were 2.22±0.45 and 1.89±0.08 in control group, which suggested a coincident trend with 99Tcm-HL91 imaging. The tumor glucose metabolism rate was also decreased which suggested a good radiotherapy outcome. Conclusions UR can effectively be used to evaluate the tumor hypoxic level in vivo. This affords a new useful method to assess the whole volume of tumor hypoxic level in clinic.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-04-06。
基金项目:陕西省科技攻关项目(2004K 13-G9(3))
通讯作者:杨爱民(Email:yangaimin@mail.xjtu.edu.cn)
更新日期/Last Update: 1900-01-01