[1]李智恒,李雨.C57小鼠受照后胸腺细胞基因表达谱的变化[J].国际放射医学核医学杂志,2007,31(4):240-244.
 LI Zhi-heng,LI Yu.Profiling of differentially expressed genes induced by ionizing radiation in thymus cells of C57 mice[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(4):240-244.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
31
期数:
2007年第4期
页码:
240-244
栏目:
放射生物学
出版日期:
1900-01-01

文章信息/Info

Title:
Profiling of differentially expressed genes induced by ionizing radiation in thymus cells of C57 mice
作者:
李智恒 李雨
200433 上海, 第二军医大学海军医学系放射医学教研室
Author(s):
LI Zhi-heng LI Yu
Deparpnent of Radiation Medicine in Navy Facility, Second Military Medical University, Shanghai 200433, China
关键词:
基因表达谱电离辐射胸腺小鼠近交C57
Keywords:
Gene expression profilingIonizing radiationThymusMice indred C57
分类号:
Q345+.2
摘要:
目的 研究受1Gyγ射线照射后一个月,C57小鼠胸腺细胞细胞基因表达谱的变化。方法 使用Agilent小鼠oligo基因芯片技术,观察受照射小鼠和非受照射小鼠两组间的基因差异表达。结果 在所观察小鼠21319个基因中,107个基因在受照射小鼠胸腺组织中表达上调2倍以上(其中13个上调4倍以上),9个基因表达下调超过200%(其中2个下调超过400%),这些变化基因涉及细胞的一些基本代谢活动。结论 变化明显的基因功能涉及胚胎发育,组织形成与维持,免疫与应激、蛋白合成、凋亡、信号转导等,上调基因的数量远多于下调基因数量,其中编码角蛋白在胚胎发育中的作用值得注意。在变化明显的上调和下调基因功能中都涉及到PI3-K,也是一个值得继续关注的现象。
Abstract:
Objective Microarrays was used to text changes of gene expression of thymus cell in C57 mice one moth after exposed to 1 Gy γ-ray irradiation. Methods The difference of gene express between irradiated and un-irradiated mice being analysis by Agilent mouse oligo microarrays. Results In the observed 21319 known genes, 107 up-regulated at least 2-fold(13 excess 4-fold); whereas 9 genes were down-regulated 2-fold(2 more 4-fold). The function of theses genes are known as some basic metabolic process. Conclusions The gene changed in the experiment involved embryo development, organ formation and maintain, immunity or stress, protein compose, apoptosis, signal transduction. The number of gene is much more than that of down-regulated. It ought to be noticed that the gens which coding homy protein in embryo development, and aught also to be noticed that the gens which involve the function of PI3-K.

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备注/Memo

备注/Memo:
收稿日期:2006-12-11。
基金项目:国家自然科学基金面上项目(3027042O)
通讯作者:李雨(E-mail:liyuyaoming@hotmail.com)
更新日期/Last Update: 1900-01-01