[1]戴光复,金月英,田源,等.冰冻切片放射自显影技术在微剂量研究中的应用[J].国际放射医学核医学杂志,2006,30(5):264-267.
　DAI Guang-fu,JIN Yue-ying,TIAN Yuan,et al.Application of study in microdosimetry with the frozen sectioning microaut-oradiogtraphy technique[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(5):264-267.
点击复制

冰冻切片放射自显影技术在微剂量研究中的应用(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
30
期数:
2006年第5期
页码:
264-267
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Application of study in microdosimetry with the frozen sectioning microaut-oradiogtraphy technique
作者:
戴光复;  金月英;  田源;  苑淑渝;  张良安
300192 天津, 中国医学科学院放射医学研究所保健物理室
Author(s):
DAI Guang-fu;  JIN Yue-ying;  TIAN Yuan;  YUAN Shu-yu;  ZHANG Liang-an
Department of Health Physics, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
辐射剂量; 冷冻超薄切片术; 放射自显影术; 碘放射性同位素; 锝
Keywords:
Radiation dosage; Cryoultramicrotomy; Autoradiography; lodine radiois; Technetium
分类号:
R144.1
摘要:
目的 用放射自显影技术观察放射性核素在细胞水平的分布。方法 采用放射自显影和冰冻切片技术,建立银颗粒密度与放射性药物强度的刻度曲线,确定放射性药物131I和99mTc的微观分布,基于核素的微观分布数据,建立微剂量的剂量估算模式。结果 银颗粒密度与施入放射性药物比活度的相关系数分别为0.9915和0.9963,刻度系数分别为1.59×10-4Bq和6.48×10-5Bq。结论 131I和99mTc在细胞水平的分布是不均匀的,银颗粒多数分布在细胞质中,因此在计算细胞水平的剂量时应考虑到其分布的不均匀性。
Abstract:
Objective To survey the distribution of radionuclide in cell level by frozen sectioning microautoradiogtraphy technique. Methods The article will describe the micro distribution of 131I and 99mTc using autoradiogtraphy and frozen sectioning technique. Results To describe the relationship between argent grain density and nuclide radioactivity a regression curve of 99mTc and 131I was depicted and the R value calculated to be 0.9915 and 0.9963 respectively, The scale coeflqcient of argent grain were 1,59×10-4Bq and 6.48×10-5Bq respectively. Conclusion The distribution of radiomedicament 131I and 99mTc at cellular level is nonunidoem.

参考文献/References:

1 戴光复,金月英,田源,等.131Ⅰ在细胞水平分布的实验研究.中华放射医学与防护杂志,2006,26(4):332-334.
2 戴光复,金月英,寇明英,等.99mTc-DTPA细胞水平分布的实验研究.中华放射医学与防护杂志,2005,25(4):319-321.
3 Flynn AA,Pedley RB,Green AJ,et al.The nonuniformity of antibody distribution in the kidney and its influence on dosimetry.Radiat Res,2003,159(2):182-189.
4 Denardo GL,Siantar CL,DeNardo SJ.Radiation dosimetry for radionuclide therapy in a nonmyeloablative strategy.Cancer Biother Radiopharm,2002,17(1):107-118.
5 戴光复,王明席,等.放射自显影术用于细胞水平微吸收剂量分布研究.中华核医学杂志,2002,22(1):55-56.

相似文献/References:

[1]邓大平,卢峰,孙洪强,等.颅脑肿瘤放射治疗时射野外器官吸收剂量体模法测量与分析[J].国际放射医学核医学杂志,2016,40(4):272.[doi:10.3760/cma.j.issn.1673-4114.2016.04.007]
　Deng Daping,Lu Feng,Sun Hongqiang,et al.Test and analysis of out-of-field organ dose in intracranial tumor radiotherapy using phantom[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):272.[doi:10.3760/cma.j.issn.1673-4114.2016.04.007]
[2]马永忠,王宏芳,冯泽臣,等.回旋加速器工作场所辐射水平的调查与分析[J].国际放射医学核医学杂志,2015,39(5):407.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 013]
　ma yongzhong,wang hongfang,feng zechen,et al.investigation and analysis of radiation dose levels in a cyclotron room[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):407.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 013]
[3]张欣,孙志娟,赵永成.医用X射线工作者回顾性剂量估算进展[J].国际放射医学核医学杂志,2016,40(5):379.[doi:10.3760/cma.j.issn.1673-4114.2016.05.009]
　Zhang Xin,Sun Zhijuan,Zhao Yongcheng.Advances in retrospective dose estimation for medical X-ray workers[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):379.[doi:10.3760/cma.j.issn.1673-4114.2016.05.009]
[4]徐佳欢,孙凯.第二代双源CT大螺距模式低辐射剂量血管成像进展[J].国际放射医学核医学杂志,2016,40(5):389.[doi:10.3760/cma.j.issn.1673-4114.2016.04.011]
　Xu Jiahuan,Sun Kai.Application of high-pitch and low-dose mode using dual-source CT by computed tomography angiography[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):389.[doi:10.3760/cma.j.issn.1673-4114.2016.04.011]
[5]郑玉明,程霞,郑棒.自制90Sr保持架在皮肤病敷贴治疗中的辐射防护作用[J].国际放射医学核医学杂志,2014,38(1):19.[doi:10.3760/cma.j.issn 1673-4114.2014.01.005]
　Zheng Yuming,Cheng Xia,Zheng Bang.Effects of serf-made 90Sr applicator retainer on radiation protection in treatment of skin disease[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(5):19.[doi:10.3760/cma.j.issn 1673-4114.2014.01.005]
[6]成钊汀,谭建.分化型甲状腺癌术后患者131I治疗的辐射剂量与防护[J].国际放射医学核医学杂志,2014,38(2):110.[doi:10.3760/cma.j.issn.1673-4114.2014.02.010]
　Cheng Zhaoting,Tan Jian.Radiation dose and protection of differentiated thyroid carcinoma postoperative patients with 131I treatment[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(5):110.[doi:10.3760/cma.j.issn.1673-4114.2014.02.010]
[7]王亮,张文艺,焦玲.CR-39固体探测器蚀刻后径迹分析和影响因素分析[J].国际放射医学核医学杂志,2014,38(2):130.[doi:10.3760/cma.j.issn.1673-4114.2014.02.013]
　Wang Liang,Zhang Wenyi,Jiao Ling.Track analysis and influencing factors analysis on CR-39 solid state detector after etching[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(5):130.[doi:10.3760/cma.j.issn.1673-4114.2014.02.013]
[8]武萍,李思进,张艳兰,等.基于30mA管电流低剂量呼吸门控PET/CT扫描条件的建立讨论[J].国际放射医学核医学杂志,2013,37(5):257.[doi:10.3760/cma.j.issn.1673-4114.2013.05.001]
　WU Ping,LI Si-jin,ZHANG Yan-lan,et al.Low-dose respiratory-gated PET/CT: based on 30 mA tube current[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):257.[doi:10.3760/cma.j.issn.1673-4114.2013.05.001]
[9]王忠周,刘素兰,王新怡,等.影响数字乳腺X线摄影辐射剂量因素研究[J].国际放射医学核医学杂志,2012,36(2):113.[doi:10.3760/cma.j.issn.1673-4114.2012.02.014]
　WANG Zhong-zhou,LIU Su-lan,WANG Xin-yi,et al.Study on factors of influence to radiation dose of digital mammography[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):113.[doi:10.3760/cma.j.issn.1673-4114.2012.02.014]
[10]张良安,张文艺,焦玲,等.《β射线所致皮肤剂量估算规范》释疑[J].国际放射医学核医学杂志,2012,36(4):238.[doi:10.3760/cma.j.issn.1673-4114.2012.04.011]
　ZHANG Liang-an,ZHA NG Wen-yi,JIAO Ling,et al.Explanation of Specifications of Skin Dose Estimagon from β Ray Exposure[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):238.[doi:10.3760/cma.j.issn.1673-4114.2012.04.011]

备注/Memo

备注/Memo:
收稿日期:2006-05-15。
基金项目:国家自然科学基金资助项目(10375091)
通讯作者:戴光复,E-mail:dgf815@126.com
更新日期/Last Update: 1900-01-01