[1]刘光伟,龚守良.siRNA与肿瘤放射治疗[J].国际放射医学核医学杂志,2004,28(4):169-172.
 LIU Guang-wei,GONG Shou-liang.siRNA and tumor radiotherapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2004,28(4):169-172.
点击复制

siRNA与肿瘤放射治疗(/HTML)
分享到:

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
28
期数:
2004年第4期
页码:
169-172
栏目:
放射医学
出版日期:
1900-01-01

文章信息/Info

Title:
siRNA and tumor radiotherapy
作者:
刘光伟 龚守良
130021 长春, 吉林大学卫生部放射生物学重点实验室
Author(s):
LIU Guang-wei GONG Shou-liang
MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China
关键词:
RNA干涉肿瘤放射治疗基因
Keywords:
RNA interferencetumorradiotherapygene
分类号:
R730.55;Q691.9
摘要:
近年来,放射肿瘤学研究者致力于采用特异性阻断剂阻断DNA修复程中关键调控基过因,并通过有效抑制DNA损伤修复,促进肿瘤细胞死亡,收到较好的肿瘤放疗效果。但是同时发现,阻断剂技术本身存在诸多缺陷,这极大地限制其尽快应用于临床肿瘤治疗。自小片段干扰核糖核酸(small interference RNA,siRNA)干涉技术发现以来,其强大的阻断真核细胞中特异表达蛋白的能力为众多研究者所关注。更重要的是,它较好地解决了应用阻断剂带来的上述问题,从而为肿瘤放射基因治疗研究提供了新途径。
Abstract:
Recently, many radio oncology researchers had gained some good curative effects on DNA damage repair inhibited and tumor cell death increased by blocking key control genes in the course of DNA repair with specific blocking agents. Otherwise, the blocking agents have many defects, which extremely limited its application on curing tumor. Since small interference RNA (siRNA) technology was discovered, its powerful function of blocking expressible specific protein in eukaryocytes was attented by many researchers. Meanwhile, this technology admirably resolved mentioned above problems caused by using the blocking agents, which provided a new way for tumor radio gene therapy.

参考文献/References:

1 Ferber D.A new way to combat therapy side effects[J].Science,1999,285(5434):1651-1653.
2 Ponder BA.Cancer genetics[J].Nature,2001,411(6835):336-341.
3 Hoeijmakers JH.Genome maintenance mechanisms for preventing cancer[J].Nature,2001,411(6835):366-374.
4 Ahlquist P.RNA-dependent RNA polymerases,viruses,and RNA silencing[J].Science,2002,296(5571):1270-1273.
5 Cullen BR.RNA interference:the new somatic cell genetics?[J].Cancer cell,2002,29(1):17-23.
6 Ardelt B,Ardelt W,Darzynkiewicz Z.Cytotoxic ribonucleases and RNA interference (RNAi)[J].Cell Cycle,2003,2(01):22-24.
7 Mcmanus MT,Sharp PA.Gene silencing in mammals by small interfering RNAs[J].Nat Rev Genet,2002,3(10):737-747.
8 Gitlin L,Karelsky S,Andino R.Short interfering RNA confers intracellular antiviral immunity in human cells[J].Nature,2002,418(6896):430-434.
9 Vanitharani R,Chellappan P,Fauquet CM.Short interfering RNA-mediated interference of gene expression and viral DNA accumulation in cultured plant cells[J].Proc Nati Acad Sci USA,2003,100(16):9632-9636.
10 Thijn R,Brummelkam P,Rene B.A system for stable expression of short interfering RNAs in mammalian cells[J].Science,2002.296:550-553.
11 Kiang A,Farrar E J,Kenna PF.Use of RNA interference to knockdown expression of mouse rhodopsin in Cos-7 cells[J].Investi Ophthalm ol Vis Sci,2003.44:2335-2337.
12 Vargason JM,Szittya G,Burgyan J.Size selective recognition of siRNA by an DNA silencing suppressor[J].Cell,2003,115(07):799-813.
13 Crnkovic F,Hoppeseyler F,Butz K.Induction of apoptosis in tumor cells by siRNA-mediated silencing of the livin/ML-IAP/KIAP gene[J].Oncogene,2003,22(05):8330-8336.
14 Miyagishi M,Taira K.U6 promoter-driven siRNAs with four uridine 3’ overhangs efficiently suppress targeted gene expression in mammalian cells[J].Nat Biotechnol,2002,20:497-500.
15 Spencer JC,Michael JS,William GN,et al.Enhanced radiation and chemotherapy mediated cell killing of human cancer cells by small inhibitory RNA silencing of DNA repair factors[J].Cancer Res,2003.63:1550-1554.
16 Parik SS,Mol CD,Tainer JA.Base excision repair enzymes family portait integrating the structure and chemistry of an entire DNA repair pathway[J].Structure,1997.12:1543-1550.
17 Herceg Z,Wang ZQ.Functions of poly (ADP-ribose) polymerase(PARP) in DNA repair,genomic integrity and cell death[J].Mutat Res,2000,477:97-110.
18 Masutani M,Nozaki T,Nishiyama E,et al.Function of poly (ADP-ribose) polymerasein response to DNA damage:gene-disruption study in mice[J].Mol Cell Biochem,1999,193(1-2):149-152.
19 Curtin NJ,Golding BT,Griffin TJ,et al.New poly (ADP-ribose)polymerase inhibitors for chem-and radiotherapy of cancer[J].Oxford University Press,2000.99:177-198.
20 Perkin S,Sun D,Nguyen A,et al.Novel Inhibitors of poly (ADP-ribose) polymerase/PARP1 and PARP2 identified using a cell-based screen in yeast[J].Cancer Res,2001.61:4175-4183.
21 Khanna KK,Jackson SP.DNA double strand breaks:signaling,repair and the cancer connection[J].Nature Genetic,2001,27:247-254.
22 Durocher D,Jackson SP.DNA-PK,ATM and ATR as sensors of DNA damage:variations on a theme?[J].Curr Opin Cell Biol,2001,27:225-231.
23 Boulton S,Kyle S,Yalcintepe L,et al.Wortmannin is a potent inhibitor of DNA double strand break but not signal strand break repair in Chinese hamster ovary cells[J].Carcinogenesis (Lond),1997.17:2285-2290.
24 Sarkaria JN,Bus by EC,Tibbetts RS,et al.Inhibition of ATM and ATR kinase actibity by the radiaosensitizing agent caffeine[J].Cancer Res,1999,59(17):4375-4382.
25 Zhou BB,Elledge SJ.The DNA damage response:putting checkpoints in perspective[J].Nature,2000,408(6811):433-439.
26 Hartwell LH,Kastan MB.Cell cycle control and cancer[J].Science,1994,266(5192):1821-1828.
27 Bus by EC,Leistritz DF,Abraham RT,et al.The radiosensitizing agent 7-hydroxy staurosporince (UCN-O1) inhibits the DNA damage checkpoint Chk 1[J].Cancer Res,2000,60(08):2108-2112.
28 Collis S,Swartz M,Deweese T.siRNA silencing of DNA repair factors results in enhanced radiation and chemotherapy mediated killing of human cancer cells[J].Int J Radiat Oncol,Biol,Phys,2003,57(02):S144.
29 Xu M,Myerson R,Hunt C,et al.Treatment of cells with Mrell siRNA increases radiation sensitivity and reduces heat induced radiosensitization[J].Int J Radiat Oncol,Biol,Phys,2003,57(02):S144-145.

相似文献/References:

[1]何燕,苏晋,郑晓霞,等.P-糖蛋白抑制剂在PET显像中的应用研究[J].国际放射医学核医学杂志,2016,40(1):1.[doi:10.3760/cma.j.issn.1673-4114.2016.01.001]
 He Yan,Su Jin,ZhengXiaoxia,et al.Developing P-glycoprotein inhibitor marked by PET[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):1.[doi:10.3760/cma.j.issn.1673-4114.2016.01.001]
[2]许飞,刘建军,黄钢,等.PET乏氧显像在预测肿瘤乏氧及指导临床治疗中的应用进展[J].国际放射医学核医学杂志,2016,40(1):35.[doi:10.3760/cma.j.issn.1673-4114.2016.01.008]
 Xu Fei,Liu Jianjun,Huang Gang,et al.The application of hypoxia imaging with PET in predicting tumor hypoxia and guiding clinical therapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):35.[doi:10.3760/cma.j.issn.1673-4114.2016.01.008]
[3]陈顺军,程兵.肿瘤细胞凋亡核素显像分子探针研究进展[J].国际放射医学核医学杂志,2016,40(2):149.[doi:10.3760/cma.j.issn.1673-4114.2016.02.013]
 Chen Shunjun,Cheng Bing.Progress in molecular probes of radionuclide tumor apoptosis imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):149.[doi:10.3760/cma.j.issn.1673-4114.2016.02.013]
[4]陈晓艳,张江虹,邵春林.STAT3与辐射敏感相关性的研究进展[J].国际放射医学核医学杂志,2016,40(3):191.[doi:10.3760/cma.j.issn.1673-4114.2016.03.007]
 Chen Xiaoyan,Jianghong,Shao Chunlin.Research progresses of correlation between STAT3 and radiosensitivity[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):191.[doi:10.3760/cma.j.issn.1673-4114.2016.03.007]
[5]赵舒怡,储小飞,樊赛军.血清肿瘤标志物与肿瘤放疗疗效评估的研究进展[J].国际放射医学核医学杂志,2015,39(5):427.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 018]
 zhao shuyi,chu xiaofei,fan saijun..progression of study on serum tumor markers in evaluation of tumor radiotherapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):427.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 018]
[6]赵徵鑫,翟贺争,张文艺,等.质子重离子治疗肿瘤的进展[J].国际放射医学核医学杂志,2016,40(5):384.[doi:10.3760/cma.j.issn.1673-4114.2016.05.010]
 Zhao Zhixin,Zhai Hezheng,Zhang Wenyi,et al.Development of proton heavy ion in tumor therapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):384.[doi:10.3760/cma.j.issn.1673-4114.2016.05.010]
[7]任佳忠,李永梅,刘岩,等.99Tcm-MDP骨显像胸部异常放射性摄取的原因分析[J].国际放射医学核医学杂志,2016,40(6):459.[doi:10.3760/cma.j.issn.1673-4114.2016.06.011]
 Jiazhong,Li Yongmei,Liu Yan,et al.Reasons for the abnormal 99Tcm-MDP uptake in the thoracic tissue on bone scintigraphy[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):459.[doi:10.3760/cma.j.issn.1673-4114.2016.06.011]
[8]马彦云,张辉.磁共振体素内不相干运动扩散加权成像的原理及应用进展[J].国际放射医学核医学杂志,2016,40(6):469.[doi:10.3760/cma.j.issn.1673-4114.2016.06.013]
 Ma Yanyun,Zhang Hui.The basic principle and application progress of intravoxel incoherent motion imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):469.[doi:10.3760/cma.j.issn.1673-4114.2016.06.013]
[9]杨卫东,汪静.肿瘤核素靶向治疗新进展[J].国际放射医学核医学杂志,2015,39(1):19.[doi:10.3760/cma.j.issn.1673-4114.2015.01.006]
 Yang Weidong,Wang Jing.Advance progress of radionuclide target tumor therapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):19.[doi:10.3760/cma.j.issn.1673-4114.2015.01.006]
[10]徐金苹,袁德晓,张江虹,等.辐射诱导的外泌体在肿瘤细胞侵袭转移中的作用[J].国际放射医学核医学杂志,2015,39(2):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]
 Xu Jinping,Yuan Dexiao,Zhang Jianghong,et al.The role of radiation-induced exosomes in tumor invasion and metastasis[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):144.[doi:10.3760/cma.j.issn.1673-4114.2015.02.009]

备注/Memo

备注/Memo:
收稿日期:2004-01-17。
更新日期/Last Update: 1900-01-01