[1]王芹,刘晓秋,李进,等.白细胞介素21基因联合不同剂量γ射线照射对乳腺癌细胞生长的影响[J].国际放射医学核医学杂志,2012,36(2):94-96.[doi:10.3760/cma.j.issn.1673-4114.2012.02.009]
　WANG Qin,LIU Xiao-qiu,LI Jin,et al.Effects of interleukin-21 gene combined with different doses of γ-ray radiation on growth of breast carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):94-96.[doi:10.3760/cma.j.issn.1673-4114.2012.02.009]
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白细胞介素21基因联合不同剂量γ射线照射对乳腺癌细胞生长的影响(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第2期
页码:
94-96
栏目:
放射生物学
出版日期:
2012-03-25

文章信息/Info

Title:
Effects of interleukin-21 gene combined with different doses of γ-ray radiation on growth of breast carcinoma cells
作者:
王芹1;  刘晓秋1;  李进1;  荣庆林2;  宋力1;  刘强1;  张恒1;  樊飞跃1
1. 天津市分子核医学重点实验室, 北京协和医学院中国医学科学院放射医学研究所, 天津 300192;
2. 天津医科大学第二医院放疗科, 天津 300211
Author(s):
WANG Qin1;  LIU Xiao-qiu1;  LI Jin1;  RONG Qing-lin2;  SONG Li1;  LIU Qiang1;  ZHANG Heng1;  FAN Fei-yue1
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
乳腺肿瘤; 基因; 白细胞介素21; 放射疗法; 基因疗法
Keywords:
Breast neoplasms; Gene; Interleukin-21; Radiotherapy; Gene therapy
DOI:
10.3760/cma.j.issn.1673-4114.2012.02.009
摘要:
目的 研究人白细胞介素21基因重组腺病毒载体(Ad-IL-21)联合不同剂量γ射线照射对人乳腺癌细胞体外生长的抑制作用.方法 采用随机数字表法设立空白对照组、β-半乳糖苷酶基因重组腺病毒载体(Ad-LacZ)对照组、Ad-IL-21组、γ射线照射组和Ad-IL-21联合γ射线照射组(联合照射组),将Ad-IL-21于体外转染人乳腺癌MCF-7细胞,转染6h后进行0~10 Gy 137Cs γ射线照射,用噻唑蓝法检测MCF-7细胞的生长抑制率.结果 Ad-IL-21转染MCF-7细胞后,MCF-7细胞生长受到的抑制效应显著高于Ad-lacZ组(F=26.34,P<0.05),单独照射组和联合照射组的抑制率均显著高于Ad-IL-21组(F=23.51,F=27.55,P均<0.05),随着照射剂量的增大,细胞抑制率逐渐增高.同一照射剂量中,联合照射组对MCF-7细胞的抑制作用均显著高于γ射线照射组,联合照射组抑制率最高,与Ad-IL-21组和γ射线照射组比较,差异具有统计学意义(F=35.68,F=38.67,P均<0.05).结论 IL-21基因联合γ射线照射对乳腺癌细胞的抑瘤作用具有协同效应,有效地抑制肿瘤细胞的生长。
Abstract:
Objective To study the effect of recombinant adenovirus vector carrying interleukin-21 gene (Ad-IL-21) combined with different doses of γ-ray radiation on the growth of breast neoplasms.Methods MCF-7 cells were transfected by Ad-IL-21 and exposed from 0 to 10 Gy 137Cs γ-ray 6 h after transfection.The cohorts were divided into 5 groups:blank control group,adenovirus vector containing β-galactosidase gene (Ad-LacZ) group,Ad-IL-21 group,γ-ray radiation group and Ad-IL-21 combined with radiation group.The inhibition rates of MCF-7 cells were detected by MTT assay.Results MCF-7 cells transfected with AdIL-21 were inhibited significantly greater than that of Ad-LacZ(F=26.34,P<0.05).The inhibition rates of γ-ray radiation group and Ad-IL-21 combined with radiation group were significantly higher than that of AdIL-21 group (F=23.51,F=27.55,both P<0.05) and the inhibition rate increased when γ-ray radiation doses increased.The inhibition rate of Ad-IL-21 combined with radiation group was higher than that of γ-ray radiation group at the same dose.The inhibition rate of Ad-IL-21 combined with radiation group was the highest,which was significantly higher than that of Ad-IL-21 group and γ ray radiation group (F=35.68,F=38.67,both P<0.05).Conclusion The recombinant Ad-IL-21 transfection combined with γ-ray radiation shows the synergism for the inhibition of the growth of MCF-7 cells.

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备注/Memo

备注/Memo:
收稿日期:2012-02-01。
基金项目:天津市自然科学基金(09JCYBJC09300)
通讯作者:樊飞跃,Email:faithyfan@yahoo.com
更新日期/Last Update: 1900-01-01