[1]李佳,王月英,邢志伟,等.角质细胞生长因子对受照小鼠的遗传保护作用[J].国际放射医学核医学杂志,2013,37(4):196-198,220.[doi:10.3760/cma.j.issn.1673-4114.2013.04.002]
　LI Jia,WANG Yue-ying,XING Zhi-wei,et al.Keratinocyte growth factor pretreatment prevents radiation-induced genetics damage in a mouse model[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(4):196-198,220.[doi:10.3760/cma.j.issn.1673-4114.2013.04.002]
点击复制

角质细胞生长因子对受照小鼠的遗传保护作用(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第4期
页码:
196-198,220
栏目:
论著
出版日期:
2013-07-25

文章信息/Info

Title:
Keratinocyte growth factor pretreatment prevents radiation-induced genetics damage in a mouse model
作者:
李佳;  王月英;  邢志伟;  李进;  郝建秀;  于程程;  姜恩海
300192 天津, 中国医学科学院放射医学研究所, 天津市分子核医学重点实验室
Author(s):
LI Jia;  WANG Yue-ying;  XING Zhi-wei;  LI Jin;  HAO Jian-xiu;  YU Cheng-cheng;  JIANG En-hai
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese A cademy of Medical Sciences, Tianjin 300192, China
关键词:
成纤维细胞生长因子; 辐射损伤; 彗星试验; 微核试验
Keywords:
Fibroblast growth factor; Radiation injuries; Comet assay; Micronucleus tests
DOI:
10.3760/cma.j.issn.1673-4114.2013.04.002
摘要:
目的 应用单细胞凝胶电泳(SCGE)和微核实验探讨角质细胞生长因子(KGF)对受照小鼠的遗传保护作用。方法 将40只C57BL/6J小鼠随机分为空白对照组、照射对照组、KGF低(1 mg/kg)、中(2 mg/kg)、高(4 mg/kg)剂量组5组。KGF的3个给药组于照射前给药,连续3d分别给予腹腔注射1、2、4 mg/kg的KGF,1次/d。照射对照组腹腔注射磷酸盐缓冲液。给药后,空白对照组给予假照射,其他4组给予7.2 Gy 137Cs γ射线全身一次性照射。应用SCGE和微核实验检测5组小鼠的DNA损伤情况。结果 与照射对照组相比,KGF低剂量组小鼠的DNA微核率显著降低,中、高剂量组的彗星尾矩、Olive尾矩和微核率均显著降低。结论 KGF对受照小鼠有显著的遗传保护作用。
Abstract:
Objective To study the protective effect of keratinocyte growth factor (KGF) in genetics damage induced by radiation using the methods of single cell gel electrophoresis (SCGE) and micronuclei assay.Methods The C57BL/6J mice were randomly divided into five groups:control group,radiation group and low,middle,high dose groups of KGF(1,2,4 mg/kg).Drugs were administrated by intraperitoneal injection.Then control group was given sham-irradiated,and other groups were given a whole body irradiation at the dose of 7.2 Gy with γ-rays.DNA damage of different groups were detected by SCGE and micronuclei assay.Results Compared with radiation group,pretreatment with KGF can significantly decrease the tail moment and micronucleus formation.Conclusion Pretreatment with KGF can effectively protect the radiation-induced genetics damage in mouse model.

参考文献/References:

[1] 苗莉,缪竞诚. 细胞因子在急性辐射损伤治疗中的作用. 国际放射医学核医学杂志,2006,30(3):173-176.
[2] 傅成波,王宝勤. 角质细胞生长因子及其辐射防护作用. 中华放射医学与防护杂志,2001,21(2):140-142.
[3] Banáth JP, Fushiki M, Olive PL. Rejoining of DNA single-and double-strand breaks in human white blood cells exposed to ionizing radiation. Int J Radiat Biol, 1998, 73(6):649-660.
[4] Acharya S, Sanjeev G, Bhat NN, et al. The effect of electron and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human blood lymphocytes. Radiat Environ Biophys, 2009, 48(2):197-203.
[5] Szerencsi A, Kubinyi G, Váliczkó E, et al. DNA integrity of human leukocytes after magnetic resonance imaging. Int J Radiat Biol, 2013[2013-05-28]. http://informahealthcare.com/doi/abs/10.3109/09553002.2013.804962.[published online ahead of print Jun 12,2013].
[6] Nakayama F, Hagiwara A, Kimura M, et al. Evaluation of radiation-induced hair follicle apoptosis in mice and the preventive effects of fibroblast growth factor-1. Exp Dermatol, 2009, 18(10):889-892.
[7] Liu CJ, Ha XQ,Jiang JJ, et al. Keratinocyte growth factor (KGF) gene therapy mediated by an attenuated form of Salmonella typhimurium ameliorates radiation induced pulmonary injury in rats. J Radiat Res, 2011,52(2):176-184.
[8] Cai Y, Wang W, Liang H, et al. Keratinocyte growth factor pretreatment prevents radiation-induced intestinal damage in a mouse model. Scand J Gastroenterol, 2013, 48(4):419-426.
[9] Kelly RM, Highfill SL, Panoskaltsis-Mortari A, et al. Keratinocyte growth factor and androgen blockade work in concert to protect against conditioning regimen-induced thymic epithelial damage and enhance T-cell reconstitution after murine bone marrow transplantation. Blood, 2008, 111(12):5734-5744.
[10] Takeoka M, Ward WF, Pollack H, et al. KGF facilitates repair of radiation-induced DNA damage in alveolar epithelial cells. Am J Physiol, 1997, 272(6 Pt1):L1174-L1180.

相似文献/References:

[1]包明月,刘玉龙.应用间充质干细胞治疗急性辐射损伤的研究现状与进展[J].国际放射医学核医学杂志,2016,40(1):65.[doi:10.3760/cma.j.issn.1673-4114.2016.01.013]
　Bao Mingyue,Liu Yulong.Recent advances in the application of mesenchymal stem cells for treatment of acute radiation injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):65.[doi:10.3760/cma.j.issn.1673-4114.2016.01.013]
[2]贺欣,储小飞,罗丹,等.穿心莲药物对辐射损伤效应影响的初步研究[J].国际放射医学核医学杂志,2015,39(5):375.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 005]
　he xin,chu xiaofei,luo dan,et al.exploration of andrographolide effects on radiation injury[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):375.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 005]
[3]路璐,张俊伶,李德冠,等.6 gy 137cs γ射线照射对小鼠造血功能损伤的动态观察研究[J].国际放射医学核医学杂志,2015,39(5):393.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 009]
　lu lu*,zhang junling,li deguan,et al.effects of 6 gy 137cs γ-irradiattion on the hematopoietic system of mice[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):393.[doi:10. 3760 / cma. j. issn. 1673-4114. 2015. 05. 009]
[4]刘惠惠,李险峰.放射性心脏损伤诊断和防治的临床研究进展[J].国际放射医学核医学杂志,2016,40(5):374.[doi:10.3760/cma.j.issn.1673-4114.2016.05.008]
　Liu Huihui,Li Xianfeng.Clinical research progress in diagnosis,prevention and treatment of radiation-induced heart disease[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):374.[doi:10.3760/cma.j.issn.1673-4114.2016.05.008]
[5]张倩如,李海涛,田红旗.小分子辐射防护药物的研究进展[J].国际放射医学核医学杂志,2016,40(5):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
　Zhang Qianru,Li Haitao,Tian Hongqi.Small molecule compounds against radiation:research advances[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(4):394.[doi:10.3760/cma.j.issn.1673-4114.2016.05.012]
[6]王浩,沈秀,靳瑾,等.“四物汤”对辐射危害的辅助保护作用研究[J].国际放射医学核医学杂志,2015,39(2):121.[doi:10.3760/cma.j.issn.1673-4114.2015.02.004]
　Wang Hao,Shen Xiu,Jin Jin,et al.Protective activity of Siwu Decoction against radiation hazards[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):121.[doi:10.3760/cma.j.issn.1673-4114.2015.02.004]
[7]曹婉婷,李浣洋,陈雪英,等.正常组织在放射治疗中并发的迟发性损伤[J].国际放射医学核医学杂志,2015,39(3):260.[doi:10.3760/cma.j.issn.1673-4114.2015.03.016]
　Cao Wanting,Li Huanyang,Chen Xueying,et al.Late effects of radiotherapy on normal tissue[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):260.[doi:10.3760/cma.j.issn.1673-4114.2015.03.016]
[8]陈雪梅,周树云,范源,等.表没食子儿茶素没食子酸酯对131I辐射损伤所致甲减大鼠模型抗氧化体系的保护作用[J].国际放射医学核医学杂志,2015,39(4):311.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
　Chen Xuemei,Zhou Shuyun,Fan Yuan,et al.Protection of antioxidant system of EGCG on the thyroid in rat model from 131I radiation damage[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(4):311.[doi:10.3760/cma.j.issn.1673-4114.2015.04.009]
[9]李德冠,路璐,吴红英,等.G-CSF联合SB203580对4Gy照射小鼠免疫系统的作用[J].国际放射医学核医学杂志,2014,38(4):216.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
　Li Deguan,Lu Lu,Wu Hongying,et al.The effects of G-CSF combined with SB203580 on the immune system of mice received 4 Gy total body irradiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(4):216.[doi:10.3760/cma.j.issn.1673-4114.2014.04.002]
[10]张洪明,李险峰.放射性心脏损伤动物模型的研究进展[J].国际放射医学核医学杂志,2014,38(6):412.[doi:10.3760/cma.j.issn.1673-4114.2014.06.015]
　Zhang Hong-ming,Li Xian-feng.Progress in animal model of radiation-induced heart disease[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(4):412.[doi:10.3760/cma.j.issn.1673-4114.2014.06.015]

备注/Memo

备注/Memo:
收稿日期:2013-05-28。
基金项目:卫生部行业基金(201002009);中国医学科学院放射医学研究所探索基金(1315)
通讯作者:姜恩海,Email:jnhl953@yahoo.com.cn
更新日期/Last Update: 1900-01-01