[1]吴涛,安锐.分子影像报告基因系统的研究进展[J].国际放射医学核医学杂志,2010,34(4):198-202.[doi:10.3760/cma.j.issn.1673-4114.2010.04.002]
 WU Tao,AN Rui.Advances in study of molecular imaging reporter gene systems[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(4):198-202.[doi:10.3760/cma.j.issn.1673-4114.2010.04.002]
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分子影像报告基因系统的研究进展(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第4期
页码:
198-202
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Advances in study of molecular imaging reporter gene systems
作者:
吴涛 安锐
430022, 武汉, 华中科技大学同济医学院附属协和医院核医学科, 湖北省分子影像重点实验室
Author(s):
WU Tao AN Rui
Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Molecular Imaging Key Laboratory of Hubei Province, Wuhan 430022, China
关键词:
基因报告分子探针分子影像技术
Keywords:
GenesreporterMolecular probesMolecular imaging techniques
DOI:
10.3760/cma.j.issn.1673-4114.2010.04.002
摘要:
分子影像报告基因系统的应用使基因治疗从实验阶段逐渐走向了临床应用,为无创性监测治疗基因的表达及进行定量分析提供了方法学技术。目前研究最多、最成熟的仍是放射性核素报告基因系统,近年来磁共振报告基因系统和光学报告基因系统的兴起和发展,极大地丰富和完善了分子影像报告基因系统的内容,并且在与应用化学和分子生物学等多学科交叉合作的基础上,涌现出了很多新型的修饰或改良的报告基因和分子探针。该文主要概述各类报告基因系统的优缺点和发展趋势。
Abstract:
The use of molecular imaging reporter gene systems has allowed gene therapy to move from the laboratory to the clinical application, which provides methodology to monitor the expression of therapeutic gene noninvasively and achieve quantitative outcome in vivo. Recently, the radionuclide reporter gene still is the focus of many studies, but MRI and optical reporter gene have gradually played a important part in reporter gene systems. On the basis of combination of multi-subject, for example applied chemistry and molecular biology, more and more new modified reporter genes and molecular probes have spread out. This paper mainly introduces the advantages and disadvantages of reporter gene system and development trends.

参考文献/References:

[1] Chinen J,Puck JM.Successes and risks of gene therapy in primary immunodeficiencies.J Allergy Clin Immunol,2004,113(4):595-603.
[2] Rosinberg A,Khan TA,Sellke FW,et al.Therapeutic angio-genesis for myocardial ischemia.Expert Rev Cardiovasc Ther,2004,2(2):271-283.
[3] Scanlon KJ.Cancer gene therapy:challenges and opportunities.Anticancer Res,2004,24(2A):501-504.
[4] 张龙江,祁吉.分子影像学报告基因显像的研究进展.中国医学影像技术,2005,21(11):1772-1775.
[5] Buursma AR,Rutgers V,Hospers GA,et al.18F-FEAU as a radiotracer for herpes simplex virus thymidine kinase gene expression:invitro comparison with other PET tracers.Nucl Med Commun,2006,27(1):25-30.
[6] Likar Y,Dobrenkov K,Olszewska M,et al.A new acycloguanosine-specific supermutant of herpes simplex virus type 1 thymidine kinase suitable for PET imaging and suicide gene therapy for potential use in patients treated with pyrimidine-based cytotoxic drugs.J Nucl Med,2008,49(5):713-720.
[7] Likar Y,Dobrenkov K,Olszewska M,et al.PET imaging of HSV1-tk mutants with acquired specificity toward pyrimidine- and acycloguanosine-based radiotracers.Eur J Nucl Med Mol Imaging,2009,36(8):1273-1282.
[8] Najjar AM,Nishii R,Maxwell DS,et al.Molecular-genetic PET imaging using an HSV1-tk mutant reporter gene with enhanced specificity to acycloguanosine nucleoside analogs.J Nucl Med,2009,50(3):409-416.
[9] Ponomarev V,Doubrovin M,Shavrin A,et al.A human-derived reporter gene for noninvasive imaging in humans:mitochondrial thymidine kinase type 2.J Nucl Med,2007,48(5):819-826.
[10] Airaksinen AJ,Nag S,Finnema SJ,et al.[11C] cyclopropyl-FLB 457:a PET radioligand for low densities of dopamine D2 receptors.Bioorg Med Chem,2008,16(13):6467-6473.
[11] Narendran R,Frankle WG,Mason NS,et al.Positron emission tomography imaging of amphetamine-induced dopamine release in the human cortex:a comparative evaluation of the high affinity dopamine D2/3 radiotracers[11C] FLB457 and[11C] fallypride.Synapse,2009,63(6):447-461.
[12] Kummer C,Winkeler A,Dittmar C,et al.Multitracer positron emission tomographic imaging of exogenous gene expression mediated by a universal herpes simplex virus 1 amplicon vector.Mol Imaging,2007,6(3):181-192.
[13] Yuan M,Wang J,Deng J,et al.Combination therapy in A549 cells.Nucl Med Biol,2010,37(3):317-326.
[14] Jeon YH,Choi Y,Kim CW,et al.H sodium/iodide symportermediated radioiodine gene therapy enhances the killing activities of CTLs in a mouse tumor model.Mol Cancer Ther,2010,9(1):126-133.
[15] Terrovitis J,Kwok KF,Lautamäki R,et al.Ectopic expression of the sodium-iodide symporter enables imaging of transplanted cardiac stem cells in vivo by single-photon emission computed tomography or positron emission tomography.J Am Coll Cardiol,2008,52(20):1652-1660.
[16] Gilad AA,Ziv K,McMahon MT,et al.MRI reporter genes.J Nucl Med,2008,49(12):1905-1908.
[17] Zurkiya O,Ch an AW,Hu X.MagA is sufficient for producing magnetic nanoparticles in mammalian cells,making it an MRI reporter.Magn Reson Med,2008,59(6):1225-1231.
[18] McMahon MT,Gilad AA,DeLiso MA,et al.New "multicolor" polypeptide diamagnetic chemical exchange saturation transfer (DIACEST) contrast agents for MRI.Magn Reson Med,2008,60(4):803-812.
[19] Gilad AA,McMahon MT,Walczak P,et al.Artificial reporter gene providing MRI contrast based on proton exchange.Nat Biotechnol,2007,25(2):217-219.
[20] Patel D,Kell A,Simard B,et al.Cu2+-labeled,SPION loaded porous silica nanoparticles for cell labeling and multifunctional imaging probes.Biomaterials,2010,31(10):2866-2873.
[21] Yamamoto S,Imaizumi M,Kanai Y,et al.Design and performance from an integrated PET/MRI system for small animals.Ann Nucl Med,2010,24(2):89-98.
[22] Oliver SL,Zerboni L,Scmmer M,et al.Development of recombinant varicellazoster viruses expressing luciferase fusion proteins for live in vivo imaging in human skin and dorsal root ganglia xenografts.J Virol Methods,2008,154(1-2):182-193.
[23] Kang HG,Tokumasu F,Clarke M,et al.Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells.Wiley Interdiscip Rev Nanomed Nanobiotechnol,2010,2(1):48-58.
[24] Ghoroghchian PP,Therien MJ,Hammer DA.In vivo fluorescence imaging:a personal perspective.Wiley Interdiscip Rev Nanomed Nanobiotechnol,2009,1(2):156-167.
[25] Lee YE,Kopelman R.Optical nanoparticle sensors for quantitative intracellular imaging.Wiley lnterdiscip Rev Nanomed Nanobiotechnol,2009,1(1):98-110.
[26] Garofalakis A,Zacharakis G,Meyer H,et al.Three-dimensional in vivo imaging of green fluorescent protein-expressing T cells in mice with noncontact fluorescence molecular tomography.Mol Imaging,2007,6(2):96-107.
[27] Lo WC,Hsu CH,Wu AT,et al.A novel cell-based therapy for contusion spinal cord injury using GDNF-delivering NIH3T3 cells with dual reporter genes monitored by molecular imaging.J Nucl Med,2008,49(9):1512-1519.
[28] Higuchi T,Auton M,Dumler K,et al.Combined reporter gene PET and iron oxide MRI for monitoring survival and localization of transplanted cells in the rat heart.J Nucl Med,2009,50(7):1088-1094.

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备注/Memo

备注/Memo:
收稿日期:2010-04-03。
基金项目:国家自然科学基金(30770605)
通讯作者:安锐,E-mail:amuiwh@163.com
更新日期/Last Update: 1900-01-01