[1]李亚军,白人驹,高硕,等.兔VX2肺肿瘤PET-CT与血管生成的相关性研究[J].国际放射医学核医学杂志,2010,34(3):139-142.
 LI Ya-jun,BAI Ren-ju,GAO Shuo,et al.The correlation between PET-CT imaging and microvessed density in rabbit lung VX2 tumor model[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(3):139-142.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第3期
页码:
139-142
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
The correlation between PET-CT imaging and microvessed density in rabbit lung VX2 tumor model
作者:
李亚军1 白人驹1 高硕2 李彦生2 刘磊2 贾薇2 蔡莉2 邢喜玲2
1. 天津医科大学总医院放射科, 300052;
2. 天津医科大学总医院PET-CT中心, 300052
Author(s):
LI Ya-jun1 BAI Ren-ju1 GAO Shuo2 LI Yan-sheng2 LIU Lei2 JIA Wei2 CAI Li2 XING Xi-ling2
Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China
关键词:
肺肿瘤正电子发射断层显像氟脱氧葡萄糖F1811C-胆碱模型动物微血管密度
Keywords:
Lung neoplasmsPositron-emission tomopraphyFluorodeoxyglucose F1811C-cholineModelanimalRabbitsMicrovessel density
分类号:
10.3760/cma.j.issn.1673-4114.2010.03.003
摘要:
目的 探讨11C-胆碱和18F-FDG PET-CT反映兔VX2肺肿瘤血管生成的效能.方法 新西兰大白兔54只,右肺接种VX2肿瘤10~11 d后,行11C-胆碱和18F-FDG PET-CT,测量肿瘤11C-胆碱和18F-FDG最大标准化摄取值(SUVmax).同时,测量肿瘤标本大小,用免疫组化方法评价肿瘤微血管密度(MVD).然后对肿瘤胆碱SUVmax18F-FDG SUVmax与肿瘤大小、MVD进行相关性分析.结果 33只实验兔成功完成所有检查,得到Vχ2肺肿瘤的11C-胆碱SUVmax平均值为4.02±3.07(1.4~12.2),对18F-FDG的SUVmax平均值为5.70±3.45(1.0~13.0),肿瘤大小平均值为(1.68±1.61)cm3(0.13~8.00 cm3).高倍镜(200倍,0.739 mm2)视野下,肿瘤MVD平均值为(35.8±13.6)个(13~64个).经统计学分析,11C-胆碱SUVmax与肿瘤大小及MVD之间均不具有相关性;18F-FDG SUVmax与MVD(r=0.525,P=0.002)之间存在正相关关系,与肿瘤大小(r=0.335,P=0.057)之间呈临界正相关关系.结论 18F-FDG PET-CT可反映兔VX2肺肿瘤血管生成,11C-胆碱PET-CT不能反映肿瘤血管生成.
Abstract:
Objective To evaluate and compare the suitability of 11C-choline and 18F-FDG PET-CT for reflecting tumors angiogenesis. Methods Fifty-four New Zealand white rabbits which weighted 2.5~3.0 kg were used in the experiment. Under general anesthesia, a needle was transthoracically inserted into the right lung, 0.5 ml viable VX2 tumor cell suspension was slowly injected through the needle to establish the model.11C-choline and 18F-FDG PET-CT were performed after 10~11 d. The tumors SUVmax were calculated. The sections were stained with hematoxylin and eosin, and immunostained for CD34. Assessment of microvessel density (MVD) was performed by computer-assisted image analysis. The relationship 11C-choline SUVmax and 18F-FDG SUVmax with tumor size and MVD were statistically analyzed. Results Thirty-three rabbits successfully completed all imaging examinations, 11C-choline and 18F-FDG differently accumulated in all lung VX2 tumors. The mean of 11C-choline SUVmax was 4.02±3.07 (1.4~12.2), and the mean of 18F-FDG SUVmax was 5.70±3.45 (1.0~13.0). The mean size of tumor was (1.68±1.61)cm3 (0.13~8.00 cm3). Under high power microscope field of vision (200×, 0.739 mm2), the mean of MVD was 35.8±13.6 (13~64). 11C-choline SUVmax did not correlate with tumor size and MVD. 18F-FDG SUVmax significantly and positively related to MVD (r=0.525, P=0.002). There was a critical positive correlation between 18F-FDG SUVmax and tumor size (r=0.335,P=0.057). Conclusions In the rabbit VX2 lung tumor model, 18F-FDG SUVmax correlated with MVD, so 18F-FDG PET-CT could reflect tumor angiogenesis, 11C-choline SUVmax did not statistically correlate with MVD,and 11C-choline PET-CT could not reflect tumor angiogenesis.

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备注/Memo

备注/Memo:
收稿日期:2010-01-15。
通讯作者:高硕(E-mail:dr_shuogao@hotmail.com)
更新日期/Last Update: 1900-01-01