[1]王芹,杜利清,徐畅,等.白细胞介素21基因联合放射对肺癌细胞生长的协同抑制作用[J].国际放射医学核医学杂志,2014,38(3):161-163.[doi:10.3760/cma.j.issn.1673-4114.2014.02.005]
　Wang Qin,Du Liqing,Xu Chang,et al.Synergism inhibition effect of adenovirus-mediated IL-21 gene combined with ionizing radiation on the growth of pulmonary carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(3):161-163.[doi:10.3760/cma.j.issn.1673-4114.2014.02.005]
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白细胞介素21基因联合放射对肺癌细胞生长的协同抑制作用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
38
期数:
2014年第3期
页码:
161-163
栏目:
出版日期:
2014-05-25

文章信息/Info

Title:
Synergism inhibition effect of adenovirus-mediated IL-21 gene combined with ionizing radiation on the growth of pulmonary carcinoma cells
作者:
王芹;  杜利清;  徐畅;  孙志娟;  王彦;  宋力;  李进;  樊飞跃;  刘强
300192 天津, 北京协和医学院中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室
Author(s):
Wang Qin;  Du Liqing;  Xu Chang;  Sun Zhijuan;  Wang Yan;  Song Li;  Li Jin;  Fan Feiyue;  Liu Qiang
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
白细胞介素类; 肺肿瘤; 放射
Keywords:
Interleukins; Lung neoplasms; Radiation
DOI:
10.3760/cma.j.issn.1673-4114.2014.02.005
摘要:
目的 探讨白细胞介素21(IL-21)基因联合放射对肺癌A549细胞生长的协同抑制作用。方法 将肺癌A549细胞分为4组:空白对照组、Ad-IL-21组(用Ad-IL-21腺病毒转染细胞)、照射组(行6 Gy 137Cs γ射线照射)以及Ad-IL-21联合照射组(用Ad-IL-21腺病毒转染细胞后再行6 Gy 137Cs γ射线照射),观察IL-21基因对A549细胞生长的抑制效应。结果 Ad-IL-21联合照射组的A549细胞的抑制效应最强(抑制率约为40%),显著高于Ad-IL-21组(约22%)和照射组(约17%)(F=45.6、57.5,P<0.05)。Ad-IL-21联合照射组A549细胞发生明显的G2期阻滞(35.4%),与Ad-IL-21组(25.2%)和照射组(17.5%)比较,差异有统计学意义(F=16.6、32.8,P<0.05)。Ad-IL-21联合照射组凋亡细胞所占比例最高,凋亡率达到33.1%,与Ad-IL-21组(27.8%)和照射组(14.8%)相比差异有统计学意义(F=15.9、41.7,P<0.05)。结论 IL-21基因联合放射对肺癌细胞生长的抑制效应具有协同作用,可显著地抑制肿瘤细胞的生长。
Abstract:
Objective To explore the synergism inhibition effect of adenovirus-mediated inter-leukin-21(IL-21) gene combined with ionizing radiation on the growth of pulmonary carcinoma cells A549.Methods A549 cells were divided into four groups:blank control group,Ad-IL-21 group (cells were transfected with Ad-IL-21),radiation group (cells were exposed to 6 Gy137Cs γ-ray) and Ad-IL-21 combined with radiation group (cells were transfected with Ad-IL-21 before exposed to 6 Gy 137Cs γ-ray).The cell growth,cell cycle and cell apoptosis of A549 cells were observed.Results The inhibition rate of Ad-IL-21 combined with radiation group was the highest,reached 40%,which was significantly higher than that of Ad-IL-21 group (about 22%) and radiation group (about17%)(F=45.6 and 57.5,both P<0.05).Cells of Ad-IL-21 combined with radiation group were arrested at G2 phase (35.4%),significantly higher than that of Ad-IL-21 group (25.2%) and radiation group (17.5%)(F=16.6 and 32.8,both P <0.05).The cell apoptosis rate of combination group was the highest,reached 33.1% and there were significant differences compared with Ad-IL-21 group (27.8%) and ra-diation group (14.8%)(F=15.9 and 41.7,both P<0.05).Conclusion Ad-IL-21 transfection com-bined with radiation shows synergism effect on the inhibition of pulmonary carcinoma cells growth.

参考文献/References:

[1] Parrish-Novak J,Dillon SR,Nelson A,et al.Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lym-phocyte function[J].Nature,2000,408(6808):57-63.
[2] Sφndergaard H,Skak K.IL-21:roles in immunopathology and can-cer therapy[J].Tissue Antigens,2009,74(6):467-479.
[3] Castellani ML,Salini V,Frydas S,et al.The proinflammatory inter-leukin-21 elicits anti-tumor response and mediates autoimmunity[J].Int J Immunopathol Pharmacol,2006,19(2):247-251.
[4] 王芹,李进,赵欣然,等.人白细胞介素21基因重组复制缺陷型腺病毒的制备与鉴定[J].中国医科大学学报,2010,39(12):1005-1008.
[5] 张耀文,曹永珍,李进,等.基因治疗联合放射治疗恶性肿瘤[J].国际放射医学核医学杂志,2008,32(4):247-250.
[6] 范凌云,谢庆军.腺病毒载体的研究进展[J].中国生物制品学杂志,2008,21(2):153-157.
[7] 王芹,刘晓秋,李进,等.白细胞介素21基因联合不同剂量γ射线照射对乳腺癌细胞生长的影响[J].国际放射医学核医学杂志,2012,36(2):94-96.
[8] 谢大兴,冯永东,张鹏,等.不同剂量X射线对细胞周期检测点和细胞凋亡的影响[J].中华实验外科杂志,2005,22(7):798-800.

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备注/Memo

备注/Memo:
收稿日期:2013-05-26。
基金项目:天津市自然科学基金(09JCYBJC09300)
通讯作者:刘强,Email:liuqiang@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01