[1]陈凤华,李进,谭志军,等.Ad-Rb94联合γ射线照射对食管癌细胞生长的影响[J].国际放射医学核医学杂志,2010,34(2):119-121.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
　CHEN Feng-hua,LI Jin,TAN Zhi-jun,et al.Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γ-ray on growth of esophageal carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):119-121.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第2期
页码:
119-121
栏目:
放射生物学
出版日期:
1900-01-01

文章信息/Info

Title:
Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γ-ray on growth of esophageal carcinoma cells
作者:
陈凤华1;  李进1;  谭志军2;  姜恩海1;  宋力1;  王欣茹3;  王芹1
1. 北京协和医学院中国医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津, 300192;
2. 天津市第一中心医院外科, 300192;
3. 北京第二炮兵总医院检验科, 100088
Author(s):
CHEN Feng-hua1;  LI Jin1;  TAN Zhi-jun2;  JIANG En-hai1;  SONG Li1;  WANG Xin-ru3;  WANG Qin1
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China
关键词:
基因; 视网膜母细胞瘤94; 食管肿瘤; 基因疗法; 放射疗法
Keywords:
Genes; retinoblastoma 94; Esophageal neoplasms; Gene therapy; Radiotherapy
DOI:
10.3760/cma.j.issn.1673-4114.2010.02.017
摘要:
目的 探讨人视网膜母细胞瘤94基因重组腺病毒载体(Ad-Rb94)联合γ射线对人食管癌细胞体外生长的抑制作用。方法 采用随机数字表法将培养细胞分为空白对照组、β-半乳糖苷酶基因重组腺病毒载体(Ad-LacZ)对照组、Ad-Rb94组、γ射线照射组和Ad-Rb94联合γ射线照射组(联合组),Ad-LacZ和Ad-Rb94于体外转染人食管癌EC109细胞系,转染6 h后进行4 Gy 137Csγ射线照射,用噻唑蓝法检测细胞的生长抑制率。结果 Ad-Rb94组、γ射线照射组和联合组对EC109细胞生长均具有抑制作用,其中,联合组抑制率最高(40.30±4.2)%,明显高于Ad-Rb94组(18.3±0.4)%和γ射线照射组(27.40±2.9)%(χ2=7.91,χ2=5.82,P<0.05);γ射线照射组与Ad-Rb94组比较差异具有统计学意义(χ2=5.12,P<0.05).结论 Ad-Rb94联合γ射线照射对食管癌细胞的抑瘤作用具有协同效应,能有效地抑制肿瘤细胞的生长。
Abstract:
Objective To study the combined effect of exogenous recombinant adenovirus-mediated retinoblastoma gene 94(Ad-Rb94) combined with γ-ray on the growth of esophageal carcinoma cells. Methods Cell culture were randomly divided into 5 groups:control group, recombinant adenovirus vector containing(β-galactosidase gene(Ad-LacZ) group, Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group. EC 109 cells were transfected by Ad-Rb94 and exposed to 4 Gy 137Cs γ-ray irradiation 6 hours after transfection. The inhibition rate of EC 109 cells were detected by MTT assay. Results EC 109 cells transfected with Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group were all inhibited. The inhibition rate of Ad-Rb94 combined with γ-ray radiation group reached(40.30%±4.2%), significantly higher than Ad-RL94 group(18.3%±0.4%)and γ-ray radiation group(27.40%±2.9%)(χ2=7.91, χ2=5.82, P<0.05). The difference of inhibition rate between γ-ray radiation group and Ad-Rb94 group was extremely significant(χ2=5.12, P<0.05).Conclusion The recombinant Ad-Rb94 gene transfection combined with γ-ray shows the synergism for the inhibition of the growth of EC 109 cells.

参考文献/References:

[1] Li D,Day KV,Yu S,et al.The role of adenovirus-mediated retinoblastoma 94 in the treatment of head and neck cancer.Cancer Res,2002,62(16):4637-4644.
[2] Zhang X,Multani AS,Zhou JH,et al.Adenoviral-mediated retinoblastoma 94 produces rapid telomere erosion,chromosomal crisis,and caspase-dependent apoptosis in bladder cancer and immortalized human urothelial cells but not in normal urothelial cells.Cancer Res,2003,63(4):760-765.
[3] Roig JM,Molina MA,Cascante A,et al.Adenovirus-mediated retinoblastoma 94 gene transfer induces human pancreatic tumor regression in a mouse xenograft model.Clin Cancer 2004,10(4):1454-1462.
[4] 张克君,李德春,朱东明,等.重组腺病毒mIκBα基因联合γ射线治疗肝细胞癌的实验观察.中华放射医学与防护杂志,2007,10(27):454-457.
[5] 张耀文,王芹,李进,等.重组腺病毒Ad-Rb94基因联合放疗对人肝癌细胞生长的抑制作用.中国辐射卫生,2008,17(2):147-148.
[6] 王蕾,李进,姜恩海,等.人Rb94基因联合γ射线照射对K150细胞生长影响的体外研究.中华放射与防护杂志,2009,29(6):568-570.

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备注/Memo

备注/Memo:
收稿日期:2009-12-10。
基金项目:国家自然科学基金(30770638)
通讯作者:王芹(E-mail:wangqin_l005@yahoo.com.cn)
更新日期/Last Update: 1900-01-01