[1]裴之俊,张永学.报告基因显像监测干细胞治疗的研究进展[J].国际放射医学核医学杂志,2010,34(2):73-75,87.[doi:10.3760/cma.j.issn.1673-4114.2010.02.002]
 PEI Zhi-jun,ZHANG Yong-xue.Advances of reporter gene imaging monitoring stem cell therapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):73-75,87.[doi:10.3760/cma.j.issn.1673-4114.2010.02.002]
点击复制

报告基因显像监测干细胞治疗的研究进展(/HTML)
分享到:

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第2期
页码:
73-75,87
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Advances of reporter gene imaging monitoring stem cell therapy
作者:
裴之俊 张永学
华中科技大学同济医学院附属协和医院核医学科, 武汉, 430002
Author(s):
PEI Zhi-jun ZHANG Yong-xue
Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Key Laboratory of Molecular Imaging of Hubei Province, Wuhan 430022, China
关键词:
基因报告分子诊断技术干细胞移植
Keywords:
GenereporterMolecular diagnostic techniquesStem cells transplantation
DOI:
10.3760/cma.j.issn.1673-4114.2010.02.002
摘要:
干细胞移植治疗各种组织损伤或退行性疾病是目前国内外研究热点,而对干细胞移植后的活体归巢、分化及功能表达等动态变化却所知甚少,影响了干细胞治疗的进一步发展。报告基因显像是分子影像技术的重要组成部分,通过间接监测转染报告基因的表达,可以动态监测移植干细胞活体内变化过程。该文简述报告基因显像活体监测移植干细胞治疗的最新研究进展和未来发展趋势。
Abstract:
Stem cell transplantation in the treatment of various tissue damage or degenerative diseases are research hotspots both at home and abroad. However, ignorance of the homing, differentiation and functional expression of the stem cell in vivo influence the further development of stem cell therapy. As an important component of molecular imaging technology, reporter gene imaging dynamically monitors the change of stem cell in vivo via monitoring the expression of transfected reporter gene. This paper briefly describes the latest research progress and the future development trend of the monitoring of reporter gene imaging in stem cell therapy in vivo.

参考文献/References:

[1] Shanthly N,Aruva MR,Zhang K,et al.Stem cells:a regenerative pharmaceutical.Q J Nucl Med Mol Imaging, 2006,50(3):205-216.
[2] Weissleder R.Molecular imaging:exploring the next frontier.Radiology,1999,212(3):609-614.
[3] Kang JH,Chung JK.Molecular-genctic imaging based on rporter gene expression.J Nucl Med,2008,49(suppl 2):S164-S179.
[4] Sun N,Lee A,Wu JC.Long term non-invasive imaging of embryonic stem cells using reporter genes.Nat Protoc,2009,4(8):1192-1201.
[5] Willmann JK,Paulmurugan R,Rodriguez-Porcel M,et al.Imaging gene expression in human mesenchymal stem cells:from small to large animals.Radiology,2009,252(1):117-127.
[6] Love Z,Wang F,Dennis J,et al.Imaging of mesenchymal stem cell transplant by bioluminescence and PET.J Nucl Med,2007,48(12):2011-2020.
[7] Roelants V,Labar D,de Meester C,et al.Comparison between aden-oviral and retroviral vectors for the transduction of the thymidine kinase PET reporter gene in rat mesenchymal stem cells.J Nucl Med,2008,49(10:1836-1844.
[8] Terrovitis J,Kwok KF,Lautam ki R,et al Ectopic expression of the sodium-iodide symporter enables imaging of transplanted cardiac stem cells in vivo by single-photon emission computedtomography or positron emission tomography.J Am Coll Cardiol,2008,52(20):1652-1660.
[9] Hofmann M,Woller KC,Meyer GP,et al.Monitoring of bone marrow cell homing into the infracted human myocardium.Circulation,2005,111(17):2198-2202.
[10] Louie AY,Hüber MM,Ahrens ET,et al.In vivo visualization of gene expression using magnetic resonance imaging.Nat Biotechnol,2000,18(3):321-325.
[11] Amsalem Y,Mardor Y,Feinberg MS,et al.Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium.Circulation,2007,116(11 Suppl):138-145.
[12] Kirchin MA,Runge VM.Contrast agents for magnetic resonance imaging:safety update.Top Magn Reson Imaging,2003,14(5):426-435.
[13] Kostura L,Kraitchman DL,Mackay AM,et al.Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis.NMR Biomed,2004,17(7):513-517.
[14] Daldrup-Link HE,Rudelius M,Oostendorp RA,et al.Targeting of hematopoietic progenitor cells with MR contrast agents.Radiology,2003,228(3):760-767.
[15] Hill JM,Dick AJ,Raman VK,et al.Serial cardiac magnetic resonance imaging of injected mesenchy-mal stem cells.Circulation,2003,108(8):1009-1014.
[16] Gao J,Dennis JE,Muzic RF,ct al.TTic dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion.Cells Tissues Organs,2001,169(1):12-20.
[17] Hofmann M,Wollert KC,Meyer GP,et al.Monitoring of bone marrow cell homing into the infarcted human myocardium.Circulation.2005,111(17):2198-2202.
[18] Zinn KR,Chaudhuri TR.The type 2 human somatostatin receptor as a platform for reporter gene imaging.Eur J Nucl Med Imaging,2002,29(3):388-399.
[19] Chaudhuri TR,Rogers BE,Zinn KR,et al.Noninvasive dual modality imaging of ovarian cancer in mice.Eur J Nucl Med,2001,28:1179.
[20] Ray P,De A,Min JJ,et al.Imaging tri-fusion multimodality reporter gene expression in living subjects.Cancer Res,2004,64(4):1323-1330.
[21] Ponomarev V,Doubrovin M,Serganova L,et al.A novel triplemodality reporter gene for whole-body fluorescent,bioluminescent,and nuclear noninvasive imaging.Eur J Nucl Med Mol Imaging,2004,31(3):740-751.
[22] Kim YJ, Dubey P,Ray P,et al,Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene.Mol Imaging Biol,2004,6(5):331-340.
[23] Lyer M,Wu L,Carey M,et al.Two-step transcriptional amplification as a method for imaging reporter gene expression using week promoter.Proc Natl Acad Sci USA,2001,98(25):14595-14600.

相似文献/References:

[1]路璐,李德冠,张俊伶,等.RNA干扰沉默p16基因对小鼠胚胎成纤维细胞衰老的影响[J].国际放射医学核医学杂志,2014,38(5):281.[doi:10.3760/cma.j.issn.1673-4114.2014.05.001]
 Lu Lu,Li De-guan,Zhang Jun-ling,et al.Application of RNA interference vector targeting mouse p16 gene in γ-irradiation-induced mouse embryonic fibroblast[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(2):281.[doi:10.3760/cma.j.issn.1673-4114.2014.05.001]
[2]王芹,王敬敏,徐畅,等.Ad-Rb94联合照射对体外人大肠癌细胞的抑瘤作用[J].国际放射医学核医学杂志,2014,38(6):351.[doi:10.3760/cma.j.issn.1673-4114.2014.06.001]
 Wang Qin,Wang Jing-min,Xu Chang,et al.The effect of Ad-Rb94 combined with radiation on inhibiting the growth of human colorectal cancer cells in vitro[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(2):351.[doi:10.3760/cma.j.issn.1673-4114.2014.06.001]
[3]李敏,孟庆慧,胡旭东,等.B细胞易位基因2的表达水平对肿瘤细胞放射敏感性的影响[J].国际放射医学核医学杂志,2013,37(3):129.[doi:10.3760/cma.j.issn.1673-4114.2013.03.001]
 LI Ming,MENG Qing-hui,HU Xu-dong,et al.Effect of B-cell translocation gene 2 alteration on radiosensitivity of cancer cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(2):129.[doi:10.3760/cma.j.issn.1673-4114.2013.03.001]
[4]王芹,刘晓秋,李进,等.白细胞介素21基因联合不同剂量γ射线照射对乳腺癌细胞生长的影响[J].国际放射医学核医学杂志,2012,36(2):94.[doi:10.3760/cma.j.issn.1673-4114.2012.02.009]
 WANG Qin,LIU Xiao-qiu,LI Jin,et al.Effects of interleukin-21 gene combined with different doses of γ-ray radiation on growth of breast carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):94.[doi:10.3760/cma.j.issn.1673-4114.2012.02.009]
[5]李继涛,何明远,袁德晓,等.p53调控的能量代谢对辐射效应的影响[J].国际放射医学核医学杂志,2012,36(6):380.[doi:10.3760/cma.j.issn.1673-4114.2012.06.014]
 LI Ji-tao,HE Ming-yuan,YUAN De-xiao,et al.Influence of p53-regulated energy metabolism in radiation effects[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(2):380.[doi:10.3760/cma.j.issn.1673-4114.2012.06.014]
[6]陈凤华,李进,谭志军,等.Ad-Rb94联合γ射线照射对食管癌细胞生长的影响[J].国际放射医学核医学杂志,2010,34(2):119.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
 CHEN Feng-hua,LI Jin,TAN Zhi-jun,et al.Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γ-ray on growth of esophageal carcinoma cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):119.[doi:10.3760/cma.j.issn.1673-4114.2010.02.017]
[7]贾立立,樊飞跃.Gadd45与基因组稳定性[J].国际放射医学核医学杂志,2009,33(2):105.[doi:10.3760/cma.j.issn.1673-4114.2009.02.013]
 JIA Li-li,FAN Fei-yue.Gadd45 and genomic stability[J].International Journal of Radiation Medicine and Nuclear Medicine,2009,33(2):105.[doi:10.3760/cma.j.issn.1673-4114.2009.02.013]
[8]王洗,刘强.脑胶质瘤辐射敏感性相关基因的研究进展[J].国际放射医学核医学杂志,2008,32(5):311.
 WANG Xi,LIU Qiang.The progress of radiosensitive genes of human brain glioma[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(2):311.
[9]袁瑛,顾兆祥.放射性核素显像在轻度认知功能障碍中的应用价值[J].国际放射医学核医学杂志,2008,32(4):209.
 YUAN Ying,GU Zhao-xiang.Applications of radionuclide imaging in mild cognitive impairment[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(2):209.
[10]林向飞,骆丹.MDM2基因与肿瘤放射生物效应[J].国际放射医学核医学杂志,2007,31(2):118.
 LIN Xiangfei,LUO Dan.MDM2 gene and radiobiological effect in tumors[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):118.
[11]吴涛,安锐.分子影像报告基因系统的研究进展[J].国际放射医学核医学杂志,2010,34(4):198.[doi:10.3760/cma.j.issn.1673-4114.2010.04.002]
 WU Tao,AN Rui.Advances in study of molecular imaging reporter gene systems[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):198.[doi:10.3760/cma.j.issn.1673-4114.2010.04.002]
[12]周翔,尹红燕,张一帆.报告基因显像监测干细胞治疗的研究进展[J].国际放射医学核医学杂志,2010,34(5):262.[doi:10.3760/cma.j.issn.1673-4114.2010.05.002]
 ZHOU Xiang,YIN Hong-yan,ZHA NG Yi-fan.Advances of reporter gene monitoring stem cell therapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):262.[doi:10.3760/cma.j.issn.1673-4114.2010.05.002]
[13]刘影,兰晓莉,张永学.单纯疱疹病毒1-胸苷激酶报告基因显像研究进展[J].国际放射医学核医学杂志,2007,31(6):329.
 LIU Ying,LAN Xiao-li,ZHA NG Yong-xue.Advances in study of perpes simplex virus type 1-thymidine kinase reporter gene imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):329.
[14]尹小花,张永学.心血管核医学分子影像研究进展[J].国际放射医学核医学杂志,2007,31(5):261.
 YIN Xiao-hua,ZHANG Yong-xue.The research progress of nuclear medicine on cardiovascular molecular imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):261.
[15]陈虞梅,黄钢.PET报告基因显像[J].国际放射医学核医学杂志,2006,30(3):139.
 CHEN Yu-mei,HUANG Gang.Investigation progress of PET reporter gene imaging[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(2):139.

备注/Memo

备注/Memo:
收稿日期:2009-10-05。
基金项目:国家自然科学基金(30830041,30571816,30772208,30970853)
通讯作者:张永学(E-mail:zhyxl229@163.com)
更新日期/Last Update: 1900-01-01