[1]范光磊,吴翼伟,章斌.用99mTc标记胰岛素样生长因子1类似物的方法学研究[J].国际放射医学核医学杂志,2007,31(4):208-211.
　FAN Guang-lei,WU Yi-wei,ZHANG Bin.Investigations of labeling insulin-like growth factor 1 analogue with 99mTc[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(4):208-211.
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用99mTc标记胰岛素样生长因子1类似物的方法学研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
31
期数:
2007年第4期
页码:
208-211
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Investigations of labeling insulin-like growth factor 1 analogue with 99mTc
作者:
范光磊;  吴翼伟;  章斌
215006 苏州, 苏州大学附属第一医院核医学科
Author(s):
FAN Guang-lei;  WU Yi-wei;  ZHANG Bin
Department of Nuclear Medicine, The First Affiliated Hospital to Smkou Unwersity, Jiangsu Suzhou 215006, China
关键词:
胰岛素样生长因子1; 锝; 同位素标记
Keywords:
Insulin-like growth factor-1; Technetium; Isotope labeling
分类号:
R817.4
摘要:
目的 建立99mTc标记胰岛素样生长因子1类似物(IGF-1A)的方法,探讨其标记条件。方法 通过预锡化法标记IGF-1A,改变标记条件:Tween 80的用量从0~10μl,在0.25~6 h不同时间点测定标记率,SnCl2·2H2O质量浓度0.75~4 g/L,IGF-1A的用量20~100μg,淋洗液的体积10~200μl,加入生理盐水或人血清后1 h~24 h测定放化纯度,纸层析法测定标记率及胶体水平。结果 99mTc-IGF-1A最高标记率为(94.43±0.75)%,放射性胶体质量分数为(3.47±0.71)%。室温下放置6h标记率为(85.57±2.81)%,加入人血清后放置24h标记率为(54.07±3.86)%。结论 上述预锡化法进行99mTc标记IGF-1A的最佳标记方法为:浓盐酸溶解SnCl2·2H2O后用葡萄糖酸钠溶液配成3g/L,吸取100μl后加入40μl IGF-1A(2g/L);300μl Na3PO4和2μl 0.1%Tween 80混匀后加入50μl 99mTcO4-新鲜淋洗液;在IGF-1A体系中加入淋洗液体系,混匀,室温下反应0.5h;加入500μl NaH2PO4-将pH值调节到7左右。该方法简捷且具有较高标记率和良好稳定性。
Abstract:
Objective To establish a useful and stable method for labeling insulin-like growth factor I analogue(IGF-1A) with 99mTc. Methods The "pretinning" method was adopted to label IGF-1A. Several labeling conditions were tested. The volume of Tween 80 was from 0 to 10μl, the labeling efficiency was determined from 0.25 h to 6 h after labeling, SnCl2·2H20 from 0.75g/L to 4g/L, the amount of IGF-1A from 20 to 100μg, ihe volume of 99mTc perrhenate was from 10 to 200μL The in vitro stability of 99mTc-IGF-IA was analyzed by using Iranian serum or sodium chloride as challenging agent, and the labeling efficiency was determined from 1 h to 24 h after added challenging agent. Results The labeling efficiency of 99mTc-IGF-1A could reach(94.43±0.75)% and the mass fraction of radiocolloid was(3.47±0.71)%. It was(85.57±2.81)% after incubation 6h at room temperature, and was(54.07±3.86)% after incubation 24 h with human serum. Conclusions The optimum labeling method was 100μl stannous chloride(3g/L) dissolved in sodium gluconate, 40μl ICF-1A(2g/L), 300μl Na3PO4, 2μl 0.1% Tween 80, 50μl 99mTc perrhenate, incubation 0.5h at room temperature, then added 500μl NaH2PO4. This method of labeling IGF-1A with 99mTc using SnCl2·2H2O and sodium gluconate was stable and high labeling efficiency was obtained.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2006-12-23。
基金项目:江苏省苏州市社会发展基金项目(SSY0617)
通讯作者:吴翼伟(E-mail:wu_yiwei3988@msn.com)
更新日期/Last Update: 1900-01-01