[1]冯彦林,谭家驹,梁生,等.[188Re(CO)3(H2O)3]+间接标记免疫磁性纳米微粒的实验研究[J].国际放射医学核医学杂志,2007,31(4):211-214.
　FENG Yan-lin,TAN Jia-ju,LIANG Sheng,et al.Labeling of immuno-magnetic nanoparticle with[188Re(CO)3(H2O)3]+[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(4):211-214.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
31
期数:
2007年第4期
页码:
211-214
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Labeling of immuno-magnetic nanoparticle with[188Re(CO)3(H2O)3]+
作者:
冯彦林1;  谭家驹1;  梁生2;  孙静1;  吴校连1;  司建华1;  夏姣云2;  温广华1
1. 528000 广东佛山, 中山大学附属佛山医院(佛山市第一人民医院)核医学科;
2. 200000 上海, 中国科学院应用物理研究所
Author(s):
FENG Yan-lin1;  TAN Jia-ju1;  LIANG Sheng2;  SUN Jing1;  WU Xiao-lian1;  Si Jian-hua1;  XIA Jiao-yun2;  WEN Guang-hua1
1. Department of Nuclear Medcine, AfflUaed Foshan Hospital of Sun Yat-Sen University and The First People’s Hospital of Foshan, Foshan 528000, China;
2. Shanhai Institute of Applied Physics, Chinses Academy of Sciences, Shanhai 201800, China
关键词:
同位素标记; 铼; 螯合剂; 磁性纳米微粒
Keywords:
Isotope labeling; Rhenium; Chelating agents; Superparamagnetic nanoparticles
分类号:
R817.4
摘要:
目的 探讨标记单克隆抗体磁性纳米微粒的实验条件。方法 以[二(2-吡啶甲基)-氨基]-乙酸(PADA)作为双功能螯合剂,将[188Re(CO)3(H2O)3]+间接标记到耦联了单克隆抗体的磁性纳米微粒。结果 [188Re(CO)3(H2O)3]+间接标记免疫磁性纳米微粒的标记率大于80%,在小牛血清和生理盐水中48 h后稳定性仍能保持在90%以上。结论 使用PADA作为双功能螯合剂,[188Re(CO)3(H2O)3]+间接标记免疫磁性纳米微粒的标记率高,稳定性好,适于进一步体内研究。
Abstract:
Objective To investigate a fine experiment condition of indirect labeling method that Hepama-1-SPIONs(supeiparamagnetic iron oxide nanoparticles)was labelled with rhenium-188 complex([188Re(CO)3(H2O)3]+). Methods The Hepama-1-SPIONs bridged by bifunctional chelating agent 2-picolylamine-N,N-diacetic acid(PADA) was labelled rhenium-188-tricarbonyl complex prepared with high labeling yields. Results The labeling rate of [188Re(CO)3(H2O)3]+-PADA-Hepama-1-SPIONs was more than 80%, and was extremely stable in vitro, The labeling rate of 188Re-(CO)3(H2O)3-PADA-Hepama-1-SPIONs was more than 90% after 48 in calf serum and isotonic Na chloride. Conclusions 188Re-(CO)3(H2O)3-PADA-Hepama-1-SPIONs shows it’s fine stability and biological properties, and it is well worth further researching as bio-magnetically targeted radiotherapy for cancer.

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备注/Memo

备注/Memo:
收稿日期:2006-12-17。
通讯作者:冯彦林(E-mail:fylin@fsyyy.com)
更新日期/Last Update: 1900-01-01