[1]唐刚华.基因表达正电子发射断层显像[J].国际放射医学核医学杂志,2001,25(6):241-245.
　TANG Gang-hua.Positron emission tomography imaging of gene expression[J].International Journal of Radiation Medicine and Nuclear Medicine,2001,25(6):241-245.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
25
期数:
2001年第6期
页码:
241-245
栏目:
综述
出版日期:
1900-01-01

文章信息/Info

Title:
Positron emission tomography imaging of gene expression
作者:
唐刚华
510515 广东 广州, 第一军医大学南方医院南方PET中心
Author(s):
TANG Gang-hua
Nanfang PET Centre, Nan Fang Hospital, First Military Medical University, Guangdong Guangzhou 510515, China
关键词:
基因表达; 反义寡核苷酸或寡脱氧核苷酸; 报告基因; 正电子发射断层显像
Keywords:
gene expression; antisense oligonucleotides or oligodeoxynucleotides; reporter genes; positron emission tomography
分类号:
R817.4
摘要:
分子生物学与核医学的结合形成了分子核医学,基因表达正电子发射断层(PET)显像是当今分子核医学研究的热点和前沿领域之一。基因表达PET显像包括反义PET显像和报告基因PET显像,反义PET显像由于技术上的问题,远不如报告基因PET显像那样发展迅速,报告基因PET显像已广泛用于动物实验研究,可望不久的将来会用于临床研究。
Abstract:
The merging of molecular biology and nuclear medicine is developed into molecular nuclear medicine.Positron emission tomography (PET) of gene expression in molecular nuclear medicine has become an attractive area. Positron emission tomography imaging gene expression includes the antisense PET imaging and the reporte gene PET imaging. It is likely that the antisense PET imaging will lag behind the reporte rgene PET imaging because of the numerous issues that have not yet to be resolved with this approach. The reporter gene PET imaging has wide application into animal experimental research and human applications of this approach will likely be reported soon.

参考文献/References:

[1] Phelps ME.Positron emission tomography provides molecular imaging of biological processes[J].Proc Natl Acad Sci USA, 2000, 97(16):9226-9233.
[2] Phelps ME.PET:The merging of biology and imaging into molecular imaging[J].J Nucl Med, 2000, 41(4):661681.
[3] Gambhir SS, Barrio JR, Herschman HR, et al.Assay for noninvasive imaging of reporter gene expression[J].Nucl Med Biol, 1999, 26(5):481-490.
[4] Gambhir SS, Barrio JR, Herschman HR, et al.Imaging gene expression:principles and assays[J].J Nucl Cardiol, 1999, 6(2):219-233.
[5] Hnatowich DJ.Antisense and nuclear medicine[J].J Nucl Med, 1999, 40(4):693-703.
[6] Dewanjee MK, Ghafouripour AK, Kapadvanjwala M, et al.Noninvasive imaging of c-myc oncogene messenger RNA with indium-111-antisense probes in a mammary tumour-bearing mouse model[J].J Nucl Med, 1994, 35:1054-1063.
[7] Kobori N, Imahori Y, Mineura K, et al.Visualization of mRNA expression in CNS using 11C-labeled phosphorothioate oligodeoxynucleotide[J].Neuroreport, 1999, 10(14):2971-2974.
[8] Pan D, Gambhir SS, Toyokuni T, et al.Rapid synthesis of a 5’-fluorinated oligodeoxynucleotide a model antisense probe for use in imaging with positron emission tomography (PET)[J].Bioorg Med Chem Lett, 1998, 8:1317-1320.
[9] Tavitian B, Terrazzino S, Kuhnast B, et al.In vivo imaging of oligonucleotides with positron emission tomography[J].Nature Med, 1998, 4:467-471.
[10] MacLaren DC, Toyokuni T, Cherry SR, et al.PET imaging of transgene expression[J].Biol Psychiatry, 2000, 48(5):337-348.
[11] Gambhir SS, Herschman HR, Cherry SR, et al.Imaging transgene expression with radionuclide imaging technologies[J].Neoplasia, 2000, 21(1-2).118-138.
[12] Herschman HR, MacLaren DC, Iyer M, et al.Seeing is believing:non-invasive, quantitative and repetitive imaging of reporter gene expression in living animals, using positron emission to mography[J].J Neurosci Res, 2000, 59(6):699-705.
[13] Maclaren DC, Gambhir SS, Satyamurthy N, et al.Repetitive, non-invasive imaging of the dopamine D2 receptor as a reporter gene in living animals[J].Gene Ther, 1999, 6:785-791.
[14] Maclaren DC, Gambhir SS, Cherry SR, et al.Repetitive and non-invasive in vivo imaging of reporter gene expression using adenovirus delivered dopamine D2 receptor as a reporter gene and FESP as a PET reporter probe[J].J Nucl Med, 1998, 39:35P.
[15] Tjuvajev JG, Avril N, Oku T, et al.Imaging herpes virus thymidine kinase gene transfer and expression by positron emission tomography[J].Cancer Res, 1998, 58:4333-4341.
[16] de Vries EFJ, van Waarde A, Harmsen MC, et al.11C-FMAU and 18F-FHPG as PET tracers for herpes simplex virus thymidine kinase enzyme activity and human cytomegalovirus infections[J].Nucl Med Biol, 2000, 27(2):113-119.
[17] Hospers GAP, Calogero A, van Waarde A, et al.Monitoring of herpes simplex virus thymidine kinase enzynie activity using positron emission tomography[J].Cancer Res, 2000, 60:1488-1491.
[18] Alauddin MM, Conti PS.Synthesis and preliminary evaluation of 9-(4-1818F-FHBG):A new potential imaging agent for viral infection and gene therapy using PET[J].Nucl Med Biol, 1998, 25, 175-180.
[19] Yu Y, Annala AJ, Barrio JR, et al.Quantification of target gene expression by imaging reporter gene expression in living animals[J].Nature, Med, 2000, 6(8):933-937.
[20] Wunderbaldinger P, Bogdanov A, Weissleder R.New approaches for imaging in gene therapy[J].Eur J Radiol, 2000, 34(3):156-165.
[21] Gambhir SS, Barrio JR, Phelps ME, et al.Imaging adenoviral-directed reporter gene expression in living animals with positron emission tomography[J].Proc Natl Acad Sci USA, 1999, 96:2333-2338.

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备注/Memo

备注/Memo:
收稿日期:2001-1-3。
作者简介:唐刚华(1968-),男,湖南东安人,第一军医大学南方医院南方PET中心讲师,博士后,主要从事药物合成与代谢、PET药物、神经递质受体PET显像以及基因表达PET显像研究。
更新日期/Last Update: 1900-01-01