[1]陆克义,王洁,刘建中,等.自噬在X射线照射所致心肌线粒体损伤中的作用[J].国际放射医学核医学杂志,2013,37(6):333-336.[doi:10.3760/cma.j.issn.1673-4114.2013.06.002]
　LU Ke-yi,WANG Jie,LIU Jian-zhong,et al.Effects of autophagy in myocardial mitochondrial injury caused by X-ray irradiation[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(6):333-336.[doi:10.3760/cma.j.issn.1673-4114.2013.06.002]
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自噬在X射线照射所致心肌线粒体损伤中的作用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
37
期数:
2013年第6期
页码:
333-336
栏目:
论著
出版日期:
2013-11-25

文章信息/Info

Title:
Effects of autophagy in myocardial mitochondrial injury caused by X-ray irradiation
作者:
陆克义1;  王洁2;  刘建中1;  李思进1;  李险峰3
1. 山西医科大学第一医院核医学科, 太原 030001;
2. 山西医科大学第一医院神经内科, 太原 030001;
3. 山西医科大学第一医院放疗科, 太原 030001
Author(s):
LU Ke-yi1;  WANG Jie2;  LIU Jian-zhong1;  LI Si-jin1;  LI Xian-feng3
Department of Nuclear Medicine, the First Hospital of Shanxi Medical University, Taiyuan 030001, China
关键词:
线粒体; 心脏; 辐射损伤; 自噬; X线
Keywords:
Mitochondria; heart; Radiation injury; Autophagy; X-rays
DOI:
10.3760/cma.j.issn.1673-4114.2013.06.002
摘要:
目的 观察X射线照射对大鼠心肌线粒体功能的影响以及自噬在线粒体功能损伤中的作用.方法 将135只实验大鼠分成假照射组(10只)、照射组(80只)和照射+3-甲基腺嘌呤(3-MA)组(45只),假照射组接受0 Gy X射线照射,照射组给予20 Gy X射线照射,照射+3-MA组接受20 Gy X射线照射前30 min腹腔注射2 μl 3-MA.采用蛋白免疫印迹法和实时定量PCR法检测心肌细胞的LC3Ⅱ及Beclin 1蛋白表达及其mRNA水平变化,检测左心室舒张压及±dP/dtmax(左心室压力上升及下降的最大速率)等离体心功能指标.结果 与假照射组相比,照射组大鼠经20 GyX射线照射后离体心功能在21 d时出现降低;而在1 d时心肌组织线粒体功能便出现了损伤;同时,心肌细胞自噬水平在3 h、6 h、12 h、1 d、2 d、4 d及7 d时出现明显上升,与假照射组之间的差异显著,且在6 h达到最高值.与照射组相比,照射+3-MA组大鼠心肌LC3Ⅱ及Beclin 1表达水平明显下降,且心功能出现异常的时间提前至14 d.结论 X射线照射损伤大鼠心肌线粒体功能并诱导自噬发生,可能是心肌保护的重要因素.
Abstract:
Objective To observed the effects of X-ray irradiation on myocardial mitochondrial function and role of autophagy in mitochondrial injury.Methods The rats were divided into shamirradiation control group,irradiation group and irradiation+3-methyladenine (3-MA) group.Rat in shamirradiation control group accepted no irradiation,while rat in irradiation group accepted local 20 Gy X-ray irradiation in the chest,the last group accepted 20 Gy X-ray irradiation prior to intraperitoneal injection 2 μl 3-MA.Western blot and real time PCR were used to detect the expression of LC3 Ⅱ and Beclin 1 in protein and their mRNA levels.In vitro cardiac function was used to detect the changes of the left ventricular diastolic pressure (LVDP),the maximal rate of rise of pressure in the isovolumetric systolic left ventricle (+dP/dtmax)and the maximal rate of rise of pressure in the isovolumetric diastolic left ventricle (-dP/dtmax).Results Compared with the sham-irradiation control group,there were the decreased of the LVDP,+dP/dtmax and -dP/dtmax at 21 d,and myocardial mitochondrial injury at 1 d after 20 Gy irradiation.The expression of LC3 and Beclin 1 in protein and mRNA levels increased significantly at 3 h,6 h,12 h,1 d,2 d,4 d and 7 d after 20 Gy irradiation,up to peak at 6 h.Compared with irradiation group,intraperitoneal injection 2 μl 3-MA 30 min before irradiation,the expression of LC3 Ⅱ and Beclin 1 in protein decreased significantly at 6 h after irradiation,there were the decreased of the LVDP,+dP/dtmax and -dP/dtmax at 14 d,then the irradiation group for 21 days.Conclusion X-ray irradiation could injury myocardial mitochondrial function and induce autophagy in rat myocardial cell,which was an important factor of myocardial protection.

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备注/Memo

备注/Memo:
收稿日期:2012-11-30。
基金项目:国家自然科学基金(81371600);山西医科大学科技创新基金(057279);山西医科大学博士启动基金(055217)
通讯作者:李险峰,Email:lixianfeng-1xf@263.net
更新日期/Last Update: 1900-01-01