[1]王月英,吴红英,李德冠,等.小鼠不同肿瘤模型对放化疗敏感性的研究[J].国际放射医学核医学杂志,2012,36(5):289-292.[doi:10.3760/cnla.j.issn.1673-4114.2012.05.006]
　WANG Yue-ying,WU Hong-ying,LI De-guan,et al.The radiation sensitivities of different mice tumor model to radiotherapy and chemotherapy[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(5):289-292.[doi:10.3760/cnla.j.issn.1673-4114.2012.05.006]
点击复制

小鼠不同肿瘤模型对放化疗敏感性的研究(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第5期
页码:
289-292
栏目:
放射生物学
出版日期:
1900-01-01

文章信息/Info

Title:
The radiation sensitivities of different mice tumor model to radiotherapy and chemotherapy
作者:
王月英;  吴红英;  李德冠;  王小春;  宋娜玲;  路璐;  张俊伶;  孟爱民
中同医学科学院放射医学研究所, 天津市分子核医学重点实验室, 天津 300192
Author(s):
WANG Yue-ying;  WU Hong-ying;  LI De-guan;  WANG Xiao-chun;  SONG Na-ling;  LU Lu;  ZHANG Jun-ling;  MENG Ai-min
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
淋巴瘤; 肉瘤180; 肝肿瘤; 白血病L1210; 食管肿瘤; 模型; 动物; 放射疗法; 药物疗法
Keywords:
Lymphoma; Sarcoma 180; Liver neoplasms; Leukemia L1210; Esophageal neoplasms; Models; animal; Radiotherapy; Drug therapy
DOI:
10.3760/cnla.j.issn.1673-4114.2012.05.006
摘要:
目的 建立人食管癌及小鼠不同移植瘤模型,比较放、化疗对小鼠的不同肿瘤模型的抑制作用。方法 动物模型建立24 h后,将不同肿瘤细胞(淋巴瘤、肉瘤S180、肝癌H22、白血病L1210)的荷瘤IRM-2近交系小鼠随机分为对照组、照射组、环磷酰胺组,每组10只.环磷酰胺组于接种隔日腹腔注射环磷酰胺(25 mg/kg)1次,0.2 ml/只,共4次.照射组于接种后第4天进行全身1 Gy照射,每日1次,连续5d.将食管癌EC901荷瘤裸鼠随机分为对照组、照射组、5-氟尿嘧啶组,每组5只.照射组于接种后第4天和第8天对肿瘤局部进行2 Gy照射,5-氟尿嘧啶组于接种后隔日腹腔注射5-氟尿嘧啶(25 mg/kg)1次,0.2 ml/只,共4次.所有小鼠于第12天处死,解剖瘤块称重,计算抑瘤率。结果 裸鼠及IRM-2近交系小鼠皮下移植瘤模型的成瘤率均为100%,放疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210荷瘤小鼠的肿瘤有显著的抑制作用,抑瘤率分别为34.57%、32.69%、31.31%和18.32%,与对照组比较差异有统计学意义(t=4.130、3.222、3.581和2.713,P分别为<0.01、<0.01、<0.01、<0.05).放疗对食管癌EC901的抑瘤率为22.99%,但与对照组比较差异无统计学意义(t=1.235,P>0.05).化疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210、食管癌EC901荷瘤小鼠的抑瘤率分别为74.47%、72.59%、69.12%、77.53%和56.32%,与对照组比较差异有统计学意义(t=12.694、12.208、7.223、11.964和5.266,P均<0.01)。结论 放、化疗对小鼠的不同肿瘤模型均有抑制作用.由于小鼠的品系和瘤源不同,对放、化疗的敏感性也存在差异.
Abstract:
Objective To set up the models of human esophageal cancer in nude mice and the models of different xenograft tumors in IRM-2 mice,observe the effects of chemotherapy and radiotherapy on the tumors.Methods Twenty-four hours after setting up the models,all the mice carrying tumors including lymphoma,hepatic carcinoma H22,leukemia L1210 and sarcoma S180 were randomly divided into 3 groups:control group,radiotherapy group and cyclophosphamidum group,10 mice per group.Mice in cyclophosphamidum group were injected cyclophosphamidum (25 mg/kg) by intraperitoneal 4 times every other day;mice in radiotherapy group were given total body irradiation at the fourth day and last for 5 days.Mice with human esophageal cancer were randomly divided into 3 groups:control group,radiotherapy group and 5-fluorouracil group,5 mice per group.Mice in radiotherapy group were given local body irradiation at the fourth day and the eighth day.Mice in 5-fluorouracil group were injected 5-fluorouracil (25 mg/kg) by intraperitoneal 4 times every other day.All the mice were killed at the twelfth day and the rates of tumor inhibition were calculated.Results The tumor inhibitory rates of IRM-2 mice in radiotherapy group were 34.57% (lymphoma),32.69%(sarcoma S180),31.31%(hepatic carcinoma H22) and 18.32%(leukemia L1210) respectively,and there were significant difference compared with control group(t=4.130,3.222,3.581 and 2.713,P<0.01,<0.01,<0.01 and <0.05).The tumor inhibitory rates of nude mice with human esophageal cancer in radiotherapy group was 22.99%,but there was no significant difference compared with control group(t=1.235,P>0.05).The tumor inhibitory rates in radiotherapy group were 74.47%(lymphoma),72.59%(sarcoma S180),69.12%(hepatic carcinoma H22),77.53%(leukemia L1210) and 56.32%(human esophageal cancer),and there were significant difference compared with control group(t=12.694,12.208,7.223,11.964 and 5.266,all P<0.01).Conclusions There were inhibitory effects of chemotherapy and radiotherapy on all types of tumors.However,the efficiency depends on the mouse strains and the type of tumor.

参考文献/References:

[1] 曹建忠,罗京伟,徐国镇.红细胞生成素在肿瘤放疗中的研究现状[J].中华放射肿瘤学杂志,2006,15(4):267-269.
[2] 蔡学瑜,陈志哲.同种移植性小鼠白血病模型的建立及应用[J].医学综述,2007,13(16):1222-1225.
[3] Rygaard J, Povlsen CO. Heterotransplantation of a human malignant tumour to "Nude" mice. Acta Pathol Microbiol Scand, 1969, 77(4):758-760.
[4] 蒲才秀,常淑芳,孙江川,等.2种人卵巢癌裸鼠移植瘤模型的生物学特性比较研究[J].重庆医科大学学报,2011,36(11):1343-1346.
[5] 王月英,吴红英,李德冠,张恒,刘强,路璐,王小春,王汝勤,孟爱民.E838保护放化疗小鼠造血功能的疗效观察[J].陕西医学杂志,2009(11):1443-1447.
[6] 王月英,王小春,吴红英,李德冠,张恒,宋娜玲,褚丽萍,路璐,杜丽清,王彦,孟爱民.17aα-D-高炔雌二醇-3-乙酯联合γ射线照射对不同品系小鼠的抑瘤作用[J].国际放射医学核医学杂志,2012,36(2):95-98.
[7] Lin DC, Du XL, Wang MR. Protein alterations in ESCC and clinical implications:a review. Dis Esophagus, 2009, 22(1):9-20.
[8] 王小春,刘金剑,王月莫,等.SKP2表达对食管癌细胞辐射敏感性的影响[J].国际放射医学核医学杂志,2012,36(2):105-108.

相似文献/References:

[1]岳进,苏丽萍.全身MRI与PET/CT在淋巴瘤骨髓浸润诊断及预后中的作用[J].国际放射医学核医学杂志,2016,40(1):50.[doi:10.3760/cma.j.issn.1673-4114.2016.01.010]
　Yue Jin,Su Lipin.Function of whole-body MRI and PET/CT in the diagnosis and prognosis of lymphoma with bone marrow infiltration[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):50.[doi:10.3760/cma.j.issn.1673-4114.2016.01.010]
[2]尤阳,轩昂,张杰,等.淋巴瘤患者大脑静息葡萄糖代谢改变[J].国际放射医学核医学杂志,2016,40(4):255.[doi:10.3760/cma.j.issn.1673-4114.2016.04.003]
　You Yang,Xuan Ang,Zhang Jie,et al.Changes in resting-state brain glucose metabolism in patients with lymphoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(5):255.[doi:10.3760/cma.j.issn.1673-4114.2016.04.003]
[3]胡娜,吴永港,肖立志,等.18F-FDG PET/CT代谢活性参数及其在淋巴瘤中的应用[J].国际放射医学核医学杂志,2015,39(4):342.[doi:10.3760/cma.j.issn.1673-4114.2015.04.015]
　Hu Na,Wu Yonggang,Xiao Lizhi,et al.Metabolic parameters of 18F-FDG PET/CT and their application in lymphoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2015,39(5):342.[doi:10.3760/cma.j.issn.1673-4114.2015.04.015]
[4]戴娜,吴翼伟.影像学技术在霍奇金淋巴瘤诊疗中的价值[J].国际放射医学核医学杂志,2014,38(2):117.[doi:10.3760/cma.j.issn.1673-4114.2014.02.011]
　Dai Na,Wu Yiwei.The value of medical imaging methods in diagnosis and treatment of Hodgkin lymphoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2014,38(5):117.[doi:10.3760/cma.j.issn.1673-4114.2014.02.011]
[5]杨希,党春江.泌尿系统原发性结外淋巴瘤的临床及影像学表现[J].国际放射医学核医学杂志,2013,37(4):230.[doi:10.3760/cma.j.issn.1673-4114.2013.04.010]
　YANG Xi,DANG Chun-jiang.The clinical and imaging appearances of primary extranodal lymphoma of the urinary system[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):230.[doi:10.3760/cma.j.issn.1673-4114.2013.04.010]
[6]李艳梅,杨建伟,李鹏,等.单眼虹膜原发非霍奇金淋巴瘤18F-FDGPET/CT显像及疗效评价一例[J].国际放射医学核医学杂志,2013,37(5):326.[doi:10.3760/cma.j.issn.1673-4114.2013.05.017]
[7]萨日,赵红光,关锋,等.18F-FDG PET/CT在弥漫性大B细胞淋巴瘤疗效评价中的临床价值[J].国际放射医学核医学杂志,2013,37(1):9.[doi:10.3760/cma.j.issn.1673-4114.2013.01.003]
　SA Ri,ZHAO Hong-guang,GUAN Feng,et al.Clinical value of 18F-FDG PET/CT in evaluation of curative effect on diffuse large B cell lymphoma[J].International Journal of Radiation Medicine and Nuclear Medicine,2013,37(5):9.[doi:10.3760/cma.j.issn.1673-4114.2013.01.003]
[8]李倩,刘建军.18F-FDG PET-CT评价淋巴瘤疗效及预后的价值[J].国际放射医学核医学杂志,2011,35(4):220.[doi:10.3760/cma.j.issn.1673-4114.2011.04.006]
　LI Qian,LIU Jian-jun.The value of 18F-FDG PET-CT in lymphoma therapy evaluation and prognosis[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(5):220.[doi:10.3760/cma.j.issn.1673-4114.2011.04.006]
[9]崔玥婵,朱晓琳,张雪君.超声引导下穿刺活检术对颈部淋巴结诊断的现状及进展[J].国际放射医学核医学杂志,2011,35(6):369.[doi:10.3760,cma.j.issn.1673-4114.2011.06.013]
　CUI Yue-chaan,ZHU Xiao-lin,ZHANG Xue-jun.Status and advances of cervical lymph nodes diagnosis by ultrasound-guided core-needle biopsy[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(5):369.[doi:10.3760,cma.j.issn.1673-4114.2011.06.013]
[10]袁航,章斌,邓胜明,等.淋巴瘤细胞株Raji摄取18F-氟脱氧葡萄糖和18F-氟脱氧胸腺嘧啶核苷的体外实验研究[J].国际放射医学核医学杂志,2009,33(6):327.[doi:10.3760/cma.j.issn.1673-4114.2009.06.002]
　YUAN Hang,ZHANG Bin,DENG Sheng-ming,et al.In vitro study of uptaking 18F-fluorodeoxyglucose and 18-fluorodeoxythymidine in human lymphoma Raji cell[J].International Journal of Radiation Medicine and Nuclear Medicine,2009,33(5):327.[doi:10.3760/cma.j.issn.1673-4114.2009.06.002]

备注/Memo

备注/Memo:
收稿日期:2012-06-29。
基金项目:国家重点基础研究发展计划(2011CB964800G);周家自然科学基金(81072237,81102873);天津市自然科学基金(12JCQNJC09100,11JCZDJC9100)
通讯作者:孟爱民(Email:ai_min_meng@126.com)
更新日期/Last Update: 1900-01-01