[1]李雨,李瑶.基因芯片技术在放射生物学研究领域的应用[J].国际放射医学核医学杂志,2002,26(4):177-179.
　Li Yu,Li Yao.The application of radilbiological study by gene chip technique[J].International Journal of Radiation Medicine and Nuclear Medicine,2002,26(4):177-179.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
26
期数:
2002年第4期
页码:
177-179
栏目:
放射医学
出版日期:
1900-01-01

文章信息/Info

Title:
The application of radilbiological study by gene chip technique
作者:
李雨1;  李瑶2
1. 200433 上海, 第二军医大学海军医学系放射医学教研室;
2. 200433 上海, 复旦大学遗传研究所
Author(s):
Li Yu1;  Li Yao2
1. Department of Navy Radiation Medicine, Second Military Medical University, Shanghai 200433, China;
2. Institute of Genetics of Fudan University of China, Shanghai 200433, China
关键词:
基因芯片; 放射生物学; 电离辐射
Keywords:
gene chip; radiobiology; irradiation
分类号:
Q345.2;Q78
摘要:
机体对电离辐射反应非常复杂,受许多分子调节途径控制,p53就是这样的应激途径之一。已经知道它涉及100多个基因的活动,这些应激基因活动具有某种细胞特异性,DNA芯片技术可以完整地研究整个细胞或器官全部基因变化,可以通过基因分析发现对电离辐射的基因反应差异,从而建立一种新的分子放射生物学方法。应用该技术已经初步发现一些新的辐射反应基因活动,在不同基因背景这些基因活动变化很大,提示生物细胞对环境有害因素反应的复杂性及其在正常组织和肿瘤分化增殖过程中基因调控的重要意义,这种技术也可能成为肿瘤治疗和其他环境毒理研
Abstract:
The responses to ionizing radiation are complex and are regulated by a number of overlapping molecular pathways.One such stress-signaling pathway involves p53,which regulates the expression of over 100 genes already identified.It is also becoming increasingly apparent that the pattern of stress gene expression has some cell type specificity.It may be possible to exploit these differences in stress gene responsiveness as molecular markers through the use of a combined informatics and functional genomics approach.The techniques of microarray analysis potentially offer the opportunity to monitor changes in gene expression across the entire set of expressed genes in a cell or organism.It again highlights the importance of a cellular context to genotoxic stress responses;it also raises the prospect of expression pro-filing of cell lines,tissues,and tumors.Such profiles may have a predictive value in cancer therapy regimens,or identification of exposures to environmental toxins.

参考文献/References:

[1] Amundson SA, Bitterner M, Meltzer P, et al. Induction of gene express as a monitor of exposure to ionizing radiation[J]. Radiat Res, 2001, 156(5):657-661.
[2] Fomace Jr AJ, Alamo IJ and Hollander MC. DNA damageinducible transcripts in mammalian cells[J]. Proc Natl Acad Sci USA, 1988,85:8800-8804.
[3] Fomace AJ. Mammalian genes induced by radiation; activation of genes associated with growth control[J]. Ann Rev Genet, 1992, 26:507-526.
[4] Amundson SA, Myers TG and Fornace AJ. Roles for p53 in growth arrest and apoptosis:Putting on the brakes after genotoxic stress[J]. Oncogene, 1998, 17:3287-3300.
[5] Amundson SA, Bittner M, Chen YD, et al. cDNA microarray hybridization reveals complexity and heterogeneity of cellul ar genotoxic stress responses[J]. Oncogene, 1999, 18:3666-3672.
[6] Amundson SA, Shahab S, Bittner M, et al. Identincation of potential mRNA markers in peripheral blood lymphocytes for human exposure to ionizing radiation[J]. Radiat Res, 2000, 154:342-346.
[7] Venter JC, Adams MD, Myers EW, et al. The sequence of the human genome[J]. Science, 2001, 291:1304-1351.
[8] Koch PCA, Momenan R, Amundson SA, et al. Estimation of relative mRNA content by filter hybridization to a polvuridylic probe[J]. Biotechniques, 2000, 29:712-714.
[9] Scherf L, Ross DT, Waltham M, et al. A gene expression database for the molecular pharmacology of cancer[J]. Genet, 2000, 24:236-244.
[10] Tusher VG, Tibshiranni R and Chu G. Significant analysis of microarrys applied to the ionizing radiation response[J]. PNAS, 2001, 98(9):5116-5121.
[11] 李雨.辐射致癌研究进展[J].国外医学·肿瘤学分册,2000,增刊:206.

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备注/Memo

备注/Memo:
收稿日期:2002-04-26。
作者简介:李雨(1956-),男,河南开封人,博士,副教授,主要从事分子放射生物学研究;李瑶(1965-),女,四川达川人,副教授,主要从事分子生物学及基因芯片技术的研究。
更新日期/Last Update: 1900-01-01