[1]韩玲,李雨,陈杞.应用DNA芯片技术结合核技术对辐射致癌分子机制的研究[J].国际放射医学核医学杂志,2001,25(2):78-81.
　HAN Ling,LI Yu,CHEN Qi.Application of DNA chip and nuclear technology to study on molecular mechanism of radiation carcinogenesis[J].International Journal of Radiation Medicine and Nuclear Medicine,2001,25(2):78-81.
点击复制

应用DNA芯片技术结合核技术对辐射致癌分子机制的研究(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
25
期数:
2001年第2期
页码:
78-81
栏目:
综述
出版日期:
1900-01-01

文章信息/Info

Title:
Application of DNA chip and nuclear technology to study on molecular mechanism of radiation carcinogenesis
作者:
韩玲;  李雨;  陈杞
200433 上海, 第二军医大学放射医学核医学研究所
Author(s):
HAN Ling;  LI Yu;  CHEN Qi
Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China
关键词:
DNA芯片; 核技术; 辐射致癌
Keywords:
DNA chip; nuclear technology; radiation carcinogenesis
分类号:
Q52
摘要:
DNA芯片技术是新近出现的新的基因分析方法。它是将成千上万个寡核苷酸固定在约厘米大小的硅片上,将待测材料用荧光素或同位素标记,在DNA芯片上与探针杂交,通过激光共聚焦显微镜对芯片进行扫描获取杂交探针的荧光信号。该技术可用于DNA测序,转录情况分析,基因诊断与基因药物设计,突变及多肽性检测遗传作图等方面的研究。
Abstract:
One major challenge facing todays cancer researchers is the development of new approaches for the identification of carcinogens and other environmental hazards. Here, we describe the potential impact of emerging technologies for measuring gene expression profiles on carcinogen identification and on the general field of radiation carcinogenesis. An example of one of these technologies is the use of DNA chips. We provide an overview the mechanism and applications of DNA chip, and the application to study on molecular mechanism of radiation carcinogenesis.

参考文献/References:

[1] Schcena M,Shalon D,Davis W,et al.Quantitative monitoring of gene expression patterns with a complementary DNA microarray[J].Science,1995,270(5235):467-470.
[2] Wilson M,DeRisi J,Kristensen HH,et al.Exploring drug-induced alterations in gene expression in mycobacterium tuberculosis by microarray hybridization[J].Proc Natl Acad Sci USA,1999,96(22):12833.
[3] Afshari CA,Nuwaysir EF,Barrett JC.Application of complementary DNA microarray technology to carcinogen identification,toxicology,and drug safety evaluation[J].Cancer Res,1999,59(19):4759-4760.
[4] Medlin JF.Timely toxicology[J].Environ Health Perspect,1999,107(5):A256-A258.
[5] Amundson SA,Bittner M,Chen Y,et al.Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses[J].Oncogene,1999,18(24):3666-3672.
[6] Schena M,Heller RA,Theriault TP,et al.Microarrays:biotechnologys discovery platform for functional genomics[J].TIB Tech,1998,16(3):301-306.
[7] Schummer M,Ng W,Nelson PS,et al.Inhexpensive handheld device for the construction of high-densitynucleic acid arrays[J].Biol Tech,1997,23(6):1087.
[8] MatsonRS,Rampal J,Pentoney SL,et al.Biopolymer synthesis on polypropylene supports:oligonucleotide arrays[J].Anal Biochem,1995,224(1):110.
[9] Brazma A,Vilo J.Gene expression data analysis[J].FEBS Lett,2000,480(1):17-24.
[10] SchenaM,Heller RA,Theriault TP,et al.Microarrays biotechnologys discovery platform for functional genomics[J].TIB Tech,1998,16(3):301-306.
[11] Editorial.Getting hip to chip[J].Nature Genet.1998,18(3):195.
[12] DeRilsi J,Penland L,Brown PO,et al.Use of a cDNA microarray to analysis gene expression patterns in human cancer[J].Nature Genet,1996,14(4):457-460.
[13] Lipshutz D,Morris D,Chee M,et al.Using oligonucleotide probe arrays to access genetic diversity[J].Biol Tech,1995,19(3):442-447.
[14] Chee M,Yang R,Hubbell E,et al.Accessing genetics information with hig-density DNA arrays[J].Science,1996,274(5287):610-614.
[15] Hacia JG,Brody LC,Chee M S,et al.Detection of heterozygous mutations in BRCA 1 using high density oligonucleotide arrays and two-colour fluorescence analysis[J].Nature Genet,1996,14(4):441-447.
[16] Hacia JG,Makalowski W,Edgemon K,et al.Evolutionary sequence comparisons using high-density oligonucleotide arrays[J].Nature Genet,1998,18(3):155-158.
[17] Brown P,Hartwell L.Genomics and human disease-variations on variation[J].Nature Genet,1998,18(1):91-93.
[18] Gress TM,Muller-Pillasch F,Geng M,et al.A pancreatic cancer-specific expression profile[J].Oncogene,1996,13(8):1819-1830.
[19] Ramsayg G.DN A chips:state of the art[J].Nature Biotechnol,1998,16(1):40-44.
[20] Griffin TJ,Tang W,Smith LM.Genetic analysis by peptide nucleic acid affinity MALDI-TOF mass spectrometry[J].Nature Biotechnol,1997,15(3):1368.
[21] Cox R.The multi-step nature of carcinogenesis and the implications for risk analysis[J].Int J Radiat,1998,73(4):373-376.

相似文献/References:

[1]杨春平,卢圣栋.基因表达的序列分析[J].国际放射医学核医学杂志,1999,23(3):131.

备注/Memo

备注/Memo:
收稿日期:2000/9/4。
作者简介:韩玲(1961-),女,上海第二军医大学放射医学核医学研究所副教授。;李雨(1956-),男,河南开封人,上海第二军医大学放射医学教研室副教授,博士,主要从事DNA辐射损伤和修复的研究。;陈杞(1932-),男,上海第二军医大学放射医学核医学研究所教授,博士生导师。
更新日期/Last Update: 1900-01-01