[1]褚丽萍,王彦,王月英,等.多肽类肺癌显像剂的制备及其初步鉴定[J].国际放射医学核医学杂志,2010,34(3):135-138.
　CHU Li-ping,WANG Yan,WANG Yue-ying,et al.The preparation and identification of peptide imaging agent of lung cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(3):135-138.
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多肽类肺癌显像剂的制备及其初步鉴定(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第3期
页码:
135-138
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
The preparation and identification of peptide imaging agent of lung cancer
作者:
褚丽萍;  王彦;  王月英;  刘金剑;  吴红英;  刘鉴峰
中国医学科学院放射医学研究所实验核医学室, 天津市分子核医学重点实验室, 天津 300192
Author(s):
CHU Li-ping;  WANG Yan;  WANG Yue-ying;  LIU Jin-jian;  WU Hong-ying;  LIU Jian-feng
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192. China
关键词:
噬菌体展示; 肽类; 肽库; 肺肿瘤
Keywords:
Phage display; Peptides; Peptide library; Lung neoplasms
分类号:
10.3760/cma.j.issn.1673-4114.2010.03.002
摘要:
目的 通过噬菌体随机七肽文库筛选出与体内肺癌组织d:\PDF\.pdf特异结合的七肽,并制备肽类肺癌早期诊断试剂.方法 通过噬菌体展示体内筛选技术获得与肺癌特异结合的噬菌体,进行序列测定,合成特异结合的多肽,125I标记后经静脉注射入小鼠体内,观察其在小鼠Lewis肺癌模型中的分布.结果 通过4轮筛选获得了QYGQFAY七肽,该七肽经125I标记后可以很好地与肺癌组织特异结合,在体内2 h左右与肿瘤结合得最充分,而且体内代谢较快,6 h左右体内分布明显减少,基本代谢完毕.结论 该七肽可以较好地对Lewis肺癌进行显像和诊断.
Abstract:
Objective To screen in vivo lung cancer specific binding 7-peptide from T7 phage display random peptide library and prepare peptide imaging agent in early diagnosis of lung cancer. Methods Used phage display in vivo technology to get the 7-peptide phage that can bind the lung cancer specifically, then sequenced and synthesized 7-peptide. After being labeled by 125I, this 7-peptide was injected into mice via vein and the distribution in the mice tumor mold was observed. Results One 7-peptide was obtained after four rounds of screening, and the peptide could bind lung cancer tissue specifically. Metabolism of this peptide in mice was fast and imaging of lung cancer was best two hours later after injection. The distribution in vivo decreased and almost disappeared after six hours. Conclusion This 7-peptide could be used to image and diagnose of lung cancer effectively.

参考文献/References:

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　CHEN Su-yun.Application of radiolabeled RGD peptides in imaging of integrin receptor[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(3):129.

备注/Memo

备注/Memo:
收稿日期:2010-02-03。
基金项目:国家自然科学基金(30700178);天津市自然科学基金(09JCYBJC13400)
通讯作者:刘鉴峰(E-mail:lewis78@163.com)
更新日期/Last Update: 1900-01-01