[1]张丽娟,马蕊,王月英,等.免疫诱导IRM-2小鼠再生障碍性贫血模型的研究[J].国际放射医学核医学杂志,2016,40(2):125-127,138.[doi:10.3760/cma.j.issn.1673-4114.2016.02.008]
　Zhang Lijuan,Ma Rui,Wang Yueying,et al.Immune-induced aplastic anemia IRM-2 mice model research[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):125-127,138.[doi:10.3760/cma.j.issn.1673-4114.2016.02.008]
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免疫诱导IRM-2小鼠再生障碍性贫血模型的研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
40
期数:
2016年第2期
页码:
125-127,138
栏目:
出版日期:
2016-03-25

文章信息/Info

Title:
Immune-induced aplastic anemia IRM-2 mice model research
作者:
张丽娟;  马蕊;  王月英;  李德冠
300192 天津, 中国医学科学院放射医学研究所, 天津市放射医学与分子核医学重点实验室
Author(s):
Zhang Lijuan;  Ma Rui;  Wang Yueying;  Li Deguan
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China
关键词:
贫血; 再生障碍性; 模型; 动物; 免疫诱导; 评价研究
Keywords:
Anemia;  aplastic; Models;  animal; Immune induction; Evaluation studies
DOI:
10.3760/cma.j.issn.1673-4114.2016.02.008
摘要:
目的 建立IRM-2小鼠再生障碍性贫血(AA)实验动物模型,并检测其血液及相关免疫学指标。方法 IRM-2小鼠经137Cs γ射线6 Gy照射后,输入DBA/2小鼠的胸腺细胞,建成免疫介导型AA模型。实验分3组,即对照组、照射组和AA模型组,于照射后第15天检测小鼠的血常规和免疫学指标。结果 IRM-2小鼠AA模型组外周血WBC、RBC、骨髓有核细胞数和网织红细胞百分比分别为(2.38±0.53)×109/L、(8.22±0.21)×1012/L、(7.14±1.19)×106/股骨和(50.2±8.9)‰,均低于对照组[(8.23±0.41)×109/L、(10.24±0.91)×1012/L、(16.5±1.61)×106/股骨和(76.2±9.7)‰];IRM-2小鼠AA模型组免疫学指标中CD4+细胞比例[(8.91±2.55)%]低于对照组[(16.14±3.09)%],CD8+细胞比例[(13.55±2.12)%]高于对照组[(6.83±1.14)%]。IRM-2小鼠AA模型组外周血及免疫学指标与对照组比较,差异均具有统计学意义。结论 IRM-2小鼠AA动物模型的建立,对AA的研究具有较好的应用价值。
Abstract:
Objective To perform traditional blood and immunologic examinations for the pathogenesis of immune-induced aplastic anemia(AA) IRM-2 mice model. Methods IRM-2 mice were divided into three groups, namely, the control, radiation, and AA model groups. IRM-2 inbred mice in the AA model group were used to set up the AA model by whole body 137Cs γ irradiation and eye venous plexus injection with DBA/2 mice thymus cells. After 15 days of radiation, blood and bone marrow cells were counted, and immunologic detection was performed. Results The peripheral WBC, RBC, bone marrow cells, and reticulocyte[(2.38±0.53)×109/L,(8.22±0.21)×1012/L,(7.14±1.19)×106/femur, and(50.2±8.9)‰] in the AA model group were lower than those of the control group [(8.23±0.41)×109/L,(10.24±0.91)×1012/L,(16.5±1.61)×106/femur, and(76.2±9.7)‰]. CD4+ cells [(8.91±2.55)%] of the AA model group were lower than those of the control group[(16.14±3.09)%], whereas CD8+ cells [(13.55±2.12)%] were higher than those of the control group [(6.83±1.14)%]. Peripheral blood and immunological indexes of the AA model group were statistically different from those of the control group. Conclusion IRM-2 mice AA model was successfully established as a good model for the study of AA.

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备注/Memo

备注/Memo:
收稿日期:2016-01-06。
基金项目:国家自然科学基金(81372928);天津市重点基金(15JCZDJC35200);中国医学科学院放射医学研究所开发基金(1539)
通讯作者:李德冠,Email:lideguan@irm-cams.ac.cn
更新日期/Last Update: 1900-01-01