[1].Ionizing Radiation Induces HMGB1 Cyto-plasmic Translocation and Extracellular Release[J].国际放射医学核医学杂志,2016,40(2):91-99.[doi:10.3760/cma.j.issn.1673-4114.2016.02.002]
　Lili Wang,Li He,Guoqiang Bao,et al.Ionizing Radiation Induces HMGB1 Cyto-plasmic Translocation and Extracellular Release[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):91-99.[doi:10.3760/cma.j.issn.1673-4114.2016.02.002]
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
40
期数:
2016年第2期
页码:
91-99
栏目:
出版日期:
2016-03-25

文章信息/Info

Title:
Ionizing Radiation Induces HMGB1 Cyto-plasmic Translocation and Extracellular Release
Author(s):
Lili Wang1;  Li He2;  Guoqiang Bao3; 5;  Xin He4;  Saijun Fan1; 4;  Haichao Wang1; 4; 5
1. School of Radiation and Public Health, the First Affiliated Hospital of Soochow University Medical College, Suzhou 215123, China;
2. Department of Ophthalmology, School of Medicine, Emory University, Atlanta, GA 30322, USA;
3. Department of General Surgery, Tangdu Hospital, The 4th Military Medical University, Xi’an 710032, China;
4. Tianjin Key Laboratory of Radiation Medicine and Molecular Naclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China;
5. Laboratory of Emergency Medicine, Feinstein Institute for Medical Research, Manhasset, NY 11030, USA
Keywords:
X-ray; HMGB1; Tumor cells; Inflammatory response; Damage-associated molecule pattern molecules
DOI:
10.3760/cma.j.issn.1673-4114.2016.02.002
文献标志码:
A
Abstract:
Objective A nucleosomal protein, HMGB1, can be secreted by activated immune cells or passively released by dying cells, thereby amplifying rigorous inflammatory responses. In this study we aimed to test the possibility that radiation similarly induces cytoplasmic HMGB1 translocation and release. Methods Human skin fibroblast (GM0639) and bronchial epithelial (16HBE) cells and rats were exposed to X-ray radiation, and HMGB1 translocation and release were then assessed by immunocytochemistry and immunoassay, respectively. Results At a wide dose range(4.0-12.0 Gy), X-ray radiation induced a dramatic cytoplasmic HMGB1 translocation, and triggered a time- and dose-dependent HMGB1 release both in vitro and in vivo. The radiation-mediated HMGB1 release was also associated with noticeable chromosomal DNA damage and loss of cell viability. Conclusions Radiation induces HMGB1 cytoplasmic translocation and extracellular release through active secretion and passive leakage processes.

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备注/Memo

备注/Memo:
收稿日期:2016-02-27。
基金项目:National Natural Science Foundation of China(81071906, 81172127, 81572969);U.S. National Center of Complementary and Alternative Medicine(NCCAM, R01AT005076);U.S. National Institute of General Medical Sciences (NIGMS, R01GM063075)
通讯作者:Saijun Fan,Email:fansaijun@irm-cams.ac.cn
更新日期/Last Update: 2016-05-17