[1].HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells[J].国际放射医学核医学杂志,2016,40(2):85-90.[doi:10.3760/cma.j.issn.1673-4114.2016.02.001]
　Lu Lu,Qinghui Meng,Ming Cui,et al.HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells[J].International Journal of Radiation Medicine and Nuclear Medicine,2016,40(2):85-90.[doi:10.3760/cma.j.issn.1673-4114.2016.02.001]
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HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
40
期数:
2016年第2期
页码:
85-90
栏目:
出版日期:
2016-03-25

文章信息/Info

Title:
HPV16 E6/E7 Negatively Affect Radiosensitivity of Lung Cancer Cells
Author(s):
Lu Lu1;  Qinghui Meng2;  Ming Cui1;  Xiaofei Chu1;  Shuyi Zhao1;  Huiwen Xiao1;  Jiali Dong1;  Eliot Marty Rosen2;  Saijun Fan1
1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin 300192, China;
2. Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington DC 20079, USA
Keywords:
Papillomavirus;  human; Lung neoplasms; Radiosensitivity; ERK1/2 and AKT; ROS
DOI:
10.3760/cma.j.issn.1673-4114.2016.02.001
文献标志码:
A
Abstract:
Objective Lung cancer cells associated with radioresistance are likely to give rise to local recurrence and distant metastatic relapse, but little is known about its underlying mechanisms. In the present paper, the effects of the HPV16 E6 and HPV16 E7 oncoprotein on the radiosensitivity of lung cancer cell lines were investigated. Methods The HPV16 E6 or HPV16 E7 oncoprotein was expressed by a transient transfection with pcDNA3-HPV16 E6 or pcDNA3-HPV16 E7 expression vector. Human lung cancer H2179 cells and mouse lung cancer Lewis cells were exposed to a γ-ray radiation source, cellular survival was evaluated by using a colony formation assay. The expression of HPV16 oncoproteins E6/E7, extracellular signal-regulated kinases 1/2(ERK1/2) and AKT signaling was determined by Western blot assay. VEGF secretion was determined by ELISA. Results Both HPV16 oncoproteins E6 and E7 significantly decreased radiosensitivity of H2179 cells, associated with a promotion of the ERK1/2 and AKT phosphorylation. A decrease of reactive oxygen species(ROS) and an increase of VEGF levels were observed in the cells expressing the HPV16 oncoproteins E6 and E7. Furthermore, a similar reduction of radiosensitivity mediated by the HPV16 oncoproteins E6 and E7 was also observed in a mouse lung cancer Lewis cells. Conclusion The findings indicate that the HPV16 oncoproteins E6 and E7 negatively affects susceptibility of lung cancer cells to radiotherapy via regulation of the ERK1/2 and Akt signaling pathway and VEGF expression.

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备注/Memo

备注/Memo:
收稿日期:2016-03-01。
基金项目:National Natural Science Foundation of China(81071906, 81172127, 81572969);Ministry of Science and Technology Researth Institutes Development Project(2014EG150134);Technology and Development and Research Projects for Research Institutes, Ministry of Science and Technology(2014EG150134);Tianjin Science and Technology Support Plan Project(14ZCZDSY00001);PUMC Youth Fund, the Fundamental Research Funds for the Central Universities(33320140187);Institute of Radiation Medicine, Chinese Academy of Medical Sciences Research Fund (1547, 1522).
通讯作者:Saijun Fan,Email:fansaijun@irm-cams.ac.cn
更新日期/Last Update: 2016-05-17