[1]杨天-,张士猛,秦夏,等.转录反式激活因子对DNA-PKcs启动子的调控作用[J].国际放射医学核医学杂志,2012,36(1):30-34.[doi:10.3760/cma.j.issn.1673-4114.2012.01.008]
　YANG Tian-yi,ZHANG Shi-meng,QIN xia,et al.The regulation of transactivator of transcription on the activity of DNA-PKcs promoter[J].International Journal of Radiation Medicine and Nuclear Medicine,2012,36(1):30-34.[doi:10.3760/cma.j.issn.1673-4114.2012.01.008]
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转录反式激活因子对DNA-PKcs启动子的调控作用(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
36
期数:
2012年第1期
页码:
30-34
栏目:
放射生物学
出版日期:
2012-01-25

文章信息/Info

Title:
The regulation of transactivator of transcription on the activity of DNA-PKcs promoter
作者:
杨天-;  张士猛;  秦夏;  李兵;  刘晓丹;  周平坤
100850 北京, 军事医学科学院放射与辐射医学研究所
Author(s):
YANG Tian-yi;  ZHANG Shi-meng;  QIN xia;  LI Bing;  LIU Xiao-dan;  ZHOU Ping-kun
Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China
关键词:
反式激活; 辐射效应; DNA依赖蛋白激酶催化亚单位基因启动子; 染色体重构
Keywords:
Trans-activation; Radiation effects; DNA dependent protein kiuase catalytic subunit promoter; Chromatin remodeling
DOI:
10.3760/cma.j.issn.1673-4114.2012.01.008
摘要:
目的 检测人类免疫缺陷病毒转录反式激活因子(TAT)对DNA依赖蛋白激酶催化亚单位(DN A-PKcs)基因启动子活性的影响。方法 用PCR方法克隆DNA-PKcs基因启动子的系列截短体,通过DNA重组构建pGL3-basic-DN A-PKcs肩动子报告质粒载体,通过双荧光素酶报告基因检测DNA-PKcs基因的肩动子活性,采用基于乳糖抑制子和乳糖操纵子并结合绿色荧光蛋白分子荧光的大规模染色质松弛报告系统观察染色质的重构活性,并研究了电离辐射对TAT参与染色体重构的影响。结果 构建了DNA-PKcs启动子(全长区域为-939 bp~-1 bp)的系列截短体的报告质粒载体,鉴定出其核心区域为-64 bp~-1 bp。TAT能够抑制DNA-PKcs基因启动子的转录活性。TAT具有大规模的染色体松弛活性,电离辐射能抑制TAT参与染色体重构的作用。结论 TAT能抑制DNA修复基因DNA-PKcs的启动子活性,电离辐射能抑制TAT参与染色体重构的作用。
Abstract:
Objective To explore the influence of human immunodeficiency virus transactivator of transcription(TAT) on the promoter activity of DNA dependent protein kinase catalytic subunit (DNA-PKcs).Methods The truncated promoters of DNA-PKcs were cloned by PCR from the template DNA from HeLa genomic DNA,and the pGL3-basic-DNA-PKcs promoter reporter plasmids were constructed.The activity of DNA-PKcs promoters was detected by dual-luciferase reporter assay system.A Lac-repressor and Lacoperator based green fluorescent protein imaging system was used to assay the chromatin remodeling activity.Results A series of reporter plasmids harboring the truncated promoters of DNA-PKcs from -939 bp to -1 bp were constructed.The sequence of -64 bp to -1 bp was identified as a critical element for the activity of DNA-PKes promoter.TAT can suppress the activity of DNA-PKcs promoter.TAT participates in the regulation of the large scale chromatin relaxation.Ionizing radiation attenuates the activity of TAT played in the chromatin remodeling.Conclusion TAT represses the promoter activity of DNA repair protein DNA-PKcs,and also play a role of large scale chromatin remodeling which can te attenuated by ionizing radiation.

参考文献/References:

[1] Futaki, S, Suzuki T, Ohashi W, et al. Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intraeellular protein delivmy. J Biol Chem, 2001, 276(8):5836-5840.
[2] Aoki Y, Tosato G. HIV-I Tat enhances Kaposi sarcoma-associated herpesvirus(KSHV)infectivity. Blood, 2004, 104(3):810-814.
[3] Cantaluppi V, Biancone L, Boccellino M, et al. HIV type 1 Tat protein is a survival factor for Kaposi’s sarcoma and endothelial cells. AIDS Res Hum Retroviruses, 2001, 17(10):965-976.
[4] Smith GC, Jackson SP. The DNA-denpendent protein kinase. Genes Dev, 1999, 13(8):916-934.
[5] Allen C, Kurimasa A, Brenneman MA, et al. DNA-dependent protein kinase suppresses double-strand bleak-induced and spontaneous homologous reeombination. Proc Natl Acad Sci U S A, 2002, 99(6):3758-3763.
[6] Park SJ, Oh EJ, Yoo MA, et al. Involvement of DNA-dependent protein kinase in regulation of stress-induced JNK activation. DNA Cell Biol, 2001, 20(10):637-645.
[7] Sun Y, Huang YC, Xu QZ, et al. HIV-1 Tat depresses DNA-PK(CS) expression and DNA repair, and sensitizes cells to ionizing radiation. Int J Radiat Oncol Biol Phys, 2006, 65(3):842-850.
[8] 秦夏,张士猛,徐勤枝,等.一种基于GFP分子荧光显示的检测大尺度染色质松弛技术的建立.生物化学与生物物理进展,2011,38(6):558-562.
[9] Connelly MA, Zhang H, Kieleczawa J, et al. The promoters for human DNA-PKcs (PRKDC) and MCM4:divergently transcribed genes located at chromosome 8 band q11. Genomics,1998, 47(l):71-83.
[10] Jeang KT, Xiao H, Rich EA. Multifaceted activities of the HIV-I transactivator of transcription, Tat. J Biol Chem, 1999, 274(4l):28837-28840.

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备注/Memo

备注/Memo:
收稿日期:2011-05-20。
基金项目:国家自然科学基金(30970677,81071361)
通讯作者:周平坤,Email:zhoupk@ni.bmi.ac.cn
更新日期/Last Update: 1900-01-01