[1]洪智慧,周小林,石怡珍,等.131I标记抗胃泌素释放前体单链抗体的制备及其稳定性和免疫活性研究[J].国际放射医学核医学杂志,2010,34(2):107-109.[doi:10.3760/cma.j.issn.1673-4114.2010.02.013]
 HONG Zhi-hui,ZHOU Xiao-lin,SHI Yi-zhen,et al.Preparation and evaluation of 131I-anti-ProGRP(31-98) single-chain antibody fragment[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):107-109.[doi:10.3760/cma.j.issn.1673-4114.2010.02.013]
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131I标记抗胃泌素释放前体单链抗体的制备及其稳定性和免疫活性研究(/HTML)
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
34
期数:
2010年第2期
页码:
107-109
栏目:
核医学技术与方法
出版日期:
1900-01-01

文章信息/Info

Title:
Preparation and evaluation of 131I-anti-ProGRP(31-98) single-chain antibody fragment
作者:
洪智慧1 周小林2 石怡珍1 刘增礼1
1. 苏州大学附属第二医院核医学科, 215004;
2. 中国辐射防护研究院, 太原, 030006
Author(s):
HONG Zhi-hui1 ZHOU Xiao-lin2 SHI Yi-zhen1 LIU Zeng-lu1
Department of Nuclear Medicine, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
关键词:
碘放射性同位素胃泌素释放钛同位素标记免疫活性单链抗体
Keywords:
Iodine radioisotopesGastrin-releasing peptideIsotope labelingImmunocompetenceSingle chain antibody
DOI:
10.3760/cma.j.issn.1673-4114.2010.02.013
摘要:
目的 研究抗胃泌素释放前体(ProGRP(31-98))单链抗体(scFv)的131I标记方法,并对其标记后的稳定性和免疫活性进行分析。方法 采用氯胺-T法碘化标记制备131I-anti-ProGRP(31-98)scFv,凝胶柱层析法分离纯化标记产物,利用纸层析法测定标记物的标记率、放化纯和稳定性,通过细胞结合试验测定131I-anti-ProGRP(31-98)scFv的免疫活性。结果 131I-anti-ProGRP(31-98)标记率为93.35%.标记产物纯化后即刻放化纯为98.49%,在37℃水浴箱中放置24 h后放化纯为94.59%,48h后测定仍大于90%,与正常人血清充分混合24 h后放化纯为85.16%,48 h测定大于80%.131I-anti-ProGRP(31-98)scFv对人小细胞肺癌NCI-H446和肺腺癌A549细胞株的免疫结合率分别为85.36%和21.02%。结论 131I-anti-ProGRP(31-98)scFv的标记率高,且有良好的稳定性和免疫活性,有望成为高表达ProGRP的恶性肿瘤放射免疫显像及治疗药物,值得进一步深入研究。
Abstract:
Objective To study the 131I labeling methods, stability and immunological activity of anti-Progastrin-releasing peptide(3l-98) single-chain antibody(anti-ProGRP(3l-98)scFv). Methods The anti-Pro-GRP(3l-98)scFv was labeled by Chloramine-T method using 131I, and purified by gel column separation method.The labeling efficiency, radiochemical purity and stability were estimated by using paper chromatography.Immunological activity was detected with cell conjugation assay. Results The labeling efficiency and radiochemical purity of 131I-anti-ProGRP (3l-98)scFv were 93.35% and 98.49%, respectively. The radiochemical purity was 94.59% after incubation in water bath at 37℃ for 24h, and still maintained above 90% for 48 h.After incubation with healthy human serum for 48 h, the radiochemical purity was still above 80%. The immunobinding ratios of 131I-anti-ProGRP (3l-98)scFv for NCI-H446 cells and A549 cells were 85.36% and 21.02%, respectively. Conclusions 131I-anti-ProGRP(3l-98)scFv not only has high labeling efficiency and radiochemical purity, but also has good stability and keeps good immunological activity, Therefore, it could be a promising agent for radioimmunoimaging and radioimmunotherapy for the malignant tumors which highly express ProGRP.

参考文献/References:

[1] 徐巧玲,周小林,石怡珍,等.抗ProGRP(31-98)单克隆抗体E-B5的131I标记及体内生物学分布研究.中华核医学杂志,2009,29(4):274-278.
[2] 高晓天,周小林,梁克,等.抗前胃泌素释放肽单克隆抗体在小细胞肺癌诊断应用.中国热带医学,2009,9(1):51-53.
[3] Nordlund MS,Fermer C,Nilsson O,et aL Production and characterization of monoclonal antibodies for immunoassay of the lung cancer marker proGRP.Tumour Biol,2007,28(2):100-110.
[4] Yamaguchi H,Soda H,Kitazaki T,et al.Serum progastrin-releasing peptide levels followed by whole-body positron emission tomography detects early recurrence of a small-cell lung cancer.Respirology,2007,12(1):137-139.
[5] Satoh H,Kagohashi K,Kurishima K,et al.Comparison of neurone-specific enolase and pro-gastrin releasing peptide in the prognostic evaluation of small cell lung cancer patients.Clin Oncol,2006,18(9):720.
[6] 尹晓玲,彭志平,文明,等.131I标记抗肺癌单克降抗体1E2在荷瘤小鼠体内分布及对小鼠移植瘤作用.中国肿瘤临床,2008,35(23):1364-1368.
[7] 苗积生,沈毅.放射性核素标记单克隆抗体(McAb)免疫反应活性分数的测定.同位素,1996,9(2):85-88.
[8] 魏莉,罗荣城.放射性核素标记抗CD20单克隆抗体治疗B细胞淋巴瘤的研究进展.国际放射医学核医学杂志,2006,30(3):153-157.
[9] Davda JP,Jain M,Batra SK,et al.A physiologically based pharmacokinetic (PBPK) mode to characterize and predict the disposition of monoclonal antibody CC49 and its single chain Fv constructs.Int Immunopharmacol,2008,8(3):401-413.
[10] 王世真.分子核医学.2版.北京:中国协和医科大学出版社,2004:253-256.
[11] Ferl GZ,Kenanova V,Wu AM,et al,A two-tiered physiologically based model for dually labeled single-chain Fv-Fc antibody fragments.Mol Cancer Ther,2006,5(6):1550-1558.
[12] 包贺菊,李亚明,于彤,等.99Tcm标记的ND1重组单链抗体在荷人结肠癌裸鼠体内分布及放射免疫显像研究.中W临床医学影像杂志,2006,17(Suppl):41-43.
[13] 刘长征,张汝森.氯胺-T法和Iodogen法碘标记抗体的比较.中山医科大学学报,1995,16(3):43-46.

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备注/Memo

备注/Memo:
收稿日期:2009-12-16。
基金项目:苏州市科技发展计划应用基础研究(医疗卫生)项目(YJS0926)
通讯作者:刘增礼(E-mail:liuzengli@126.com)
更新日期/Last Update: 1900-01-01