[1]阳艳丽,王自正.噬菌体展示技术在肿瘤诊断和治疗中的应用[J].国际放射医学核医学杂志,2008,32(2):65-68.
　YANG Yan-li,WANG Zi-zheng.Application of phage display in diagnosis and therapy of cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(2):65-68.
点击复制

噬菌体展示技术在肿瘤诊断和治疗中的应用(/HTML)
分享到：

《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
32
期数:
2008年第2期
页码:
65-68
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Application of phage display in diagnosis and therapy of cancer
作者:
阳艳丽1;  王自正2
1. 210009 南京, 东南大学医学院临床检验诊断学;
2. 210006 南京, 南京医科大学附属南京第一医院临床核医学
Author(s):
YANG Yan-li1;  WANG Zi-zheng2
1. Clincal Laboretory Diagnostics, Medical College of Southeast University, Nanjing 210009, China;
2. Nanjing Clinical Nuclear Medicine Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China
关键词:
肽库; 药物筛选试验; 抗肿瘤; 分子诊断技术; 肿瘤辅助疗法; 噬菌体展示
Keywords:
Peptide library; Drug screening assays; antitumor; Molecular diagnostic techniques; Neoadjuvant therapy; Phage display
摘要:
噬菌体体内展示技术的出现,使器官靶向性多肽的筛选、器官特异性血管分子作图成为可能。更值得注意的是,通过动物体内的筛选,鉴定出了一系列肿瘤相关分子的标志物的特异性多肽配体,为肿瘤的诊断,肿瘤药物的输送以及肿瘤血管性基因治疗等提供了靶向性分子。最近,人们又成功地将此技术应用于肿瘤患者,并鉴定出与肿瘤特异性结合的多肽模序。
Abstract:
Phage display is possible to screening targeted peptides, mapping organ-specific vascular molecule because of in viva phage display. It is more remarkable that a variety of peptide ligands specific for tumor-associated makers is identified, which supplied targeted molecules for tumor diagnosis, delivery of tumor drugs and tumor vascular gene therapy. Recently, people succeed in applying the technology to tumor patients and identifying peptide motifs binding to tumor specifically.

参考文献/References:

[1] Hassan ME, Highsmith EW. Phage display technology:clinical applications and recent innovations[J]. Clin Biochem, 2002, 35(6):425-445.
[2] Mori T. Cancer-specificligandsidentified from screening of peptidedisplay libraries[J]. Curr Pharm Des, 2004, 10(19):2335-2343.
[3] Zitzmann S, Mier W, Schad A, et al. A new prostate carcinoma binding peptide (DUP-1) for tumor imaging and therapy[J]. Clin Cancer Res, 2005, 11(1):139-146.
[4] Weller EG, Wong MK, Modzelewsk RA, et al. Ultrasonic imaging of tumor angiogenesis using contrast mierobubbles targeted via the tumor-binding peptide arginine-arginine-leucine[J]. Cancer Res,2005, 65(2):533-539.
[5] Valadon P, Garnett JD, Testa JE, et al. Screening phage display libraries for organ-specific vascular immunotargeting in vivo[J]. Proc Natl Acad Sci USA, 2006, 103(2):407-412.
[6] Hoffman JA, Giraudo E, SinghM, et al. Progressive vascular changes in a transgenic mouse model of squamous cell carcinoma[J]. Cancer Cell, 2003, 4(5):383-391.
[7] Joyce JA, Laakkonen P, Bernasconi M, et al. Stage-specific vascular markers revealed by phage display in a mouse model of pancreatic islet tumorigenesis[J]. Cancer Cell, 2003, 4(5):393-403.
[8] Barth S, Huhn M, Matthey B, et al. Ki-4(scFv)-ETA, a new recombinant anti-CD30 immunotoxin with highly specific cytotoxic activity against disseminated Hodgkin tumors in SCID mice[J]. Blood, 2000, 95(12):3909-3914.
[9] Adams GP, Shaller CC, Dadachova E, et al. A single treatment of Yttrium-90-1abeled CHX-A"-C6.5 diabody inhibits the growth of established human tumor xenografts in immunodeficient mice[J]. Cancer Res, 2004, 64(17):6200-6206.
[10] Asano R, Sone Y, Makabe K, et al. Humanization of the bispeeifie epidermal growth factor receptor x CD3 diabody and its efficacy as a potential clinical reagent[J]. Clin Cancer Res, 2006, 12(13):4036-4042.
[11] Kerbel R, Folkman J. Clinical translation of angiogenesis inhibitors[J]. Nat Rev Cancer, 2002, 2(10):727-739.
[12] Curnis F, Sacchi A, Borgna L, et al. Enhancement of tumor necrosis factor alpha antitumor immunotherapeutic properties by targeted delivery to aminopeptidase N (CD13)[J]. Nat Biotechnol, 2000, 18(11):1185-1190.
[13] Laakkonen P, Akerman ME, Biliran H, et al. Antitumor activity of a homing peptide that targets tumor lymphatics and tumor eells[J]. Proc Natl Acad Sci USA, 2004, 101(25):9381-9386.
[14] Larocca D, Baird A. Receptor-mediated gene transfer by phagedisplay vectors:applications in functional genomics and gene therapy[J]. Drug Discov Today, 2001, 6(15):793-801.
[15] Krag DN, Fulle SP, Oligino L, et al. Phage-displayed random peptide libraries in mice:toxicity after serial panning[J]. Cancer Chemother Pharmacol, 2002, 50(4):325-332.
[16] Arap W, Kdonin MG, Trepel M, et al. Steps toward mapping the human vasculature by phage display[J]. Nat Med, 2002, 8(2):121-127.
[17] Krag DN, Shukla GS, Shen GP, et al. Selection of tumor-binding ligands in cancer patients with phage display libraries[J]. Cancer Res, 2006, 66(15):7724-7733.

相似文献/References:

[1]黄斌,俞杨,王自正.噬菌体展示肽库技术在肿瘤诊治研究中的应用[J].国际放射医学核医学杂志,2011,35(4):202.[doi:10.3760/cma.j.issn.1673-4114.2011.04.002]
　HUANG Bin,YU Yang,WANG Zi-zheng.Phage display peptide library technology’s application in the diagnosis and therapy of tumor[J].International Journal of Radiation Medicine and Nuclear Medicine,2011,35(2):202.[doi:10.3760/cma.j.issn.1673-4114.2011.04.002]
[2]褚丽萍,王彦,王月英,等.多肽类肺癌显像剂的制备及其初步鉴定[J].国际放射医学核医学杂志,2010,34(3):135.
　CHU Li-ping,WANG Yan,WANG Yue-ying,et al.The preparation and identification of peptide imaging agent of lung cancer[J].International Journal of Radiation Medicine and Nuclear Medicine,2010,34(2):135.
[3]俞杨,王自正.噬菌体展示体内筛选技术及其应用进展[J].国际放射医学核医学杂志,2008,32(1):5.
　YU Yang,WANG Zi-zheng.Phage display in vivo selection and its application[J].International Journal of Radiation Medicine and Nuclear Medicine,2008,32(2):5.

备注/Memo

备注/Memo:
收稿日期:2007-07-16。
基金项目:江苏省社会发展科技项目(BS2004507)
通讯作者:王自正(E-mail:zzwang136@yahoo.com.cn)
更新日期/Last Update: 1900-01-01