[1]薛艳,安瑞芳,喻明启,等.99mTc标记抗β-人绒毛膜促性腺激素单克隆抗体[J].国际放射医学核医学杂志,2007,31(2):68-69.
　XUE Yan,AN Rui-fang,YU Ming-qi,et al.Direct labeling of anti β-human chorionic gonadotropin monoclonal antibody with 99mTc[J].International Journal of Radiation Medicine and Nuclear Medicine,2007,31(2):68-69.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
31
期数:
2007年第2期
页码:
68-69
栏目:
实验核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Direct labeling of anti β-human chorionic gonadotropin monoclonal antibody with 99mTc
作者:
薛艳1;  安瑞芳1;  喻明启2;  王姝1;  谢丽1
1. 710061 西安, 西安交通大学第一附属医院妇产科;
2. 710061 西安, 西安交通大学第一附属医院核医学科
Author(s):
XUE Yan1;  AN Rui-fang1;  YU Ming-qi2;  WANG Shu1;  XIE Li1
1. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Medical School, Xi’an Jiaotong University, Xi’an 710061, China;
2. Department of Nuclear Medicine, The First Affiliated Hospital of Medical School, Xi’an Jiaotong University, Xi’an 710061, China
关键词:
锝; 绒毛膜促性腺激素; β亚单位; 人; 抗体; 抗独特性; 同位素标记
Keywords:
99mTc; Chorionic gonadotropin;  beta subunit;  human; Antibody;  anti-diotypic; Isotope Iabeling
分类号:
R817.9
摘要:
目的 用99mTc标记抗β-人绒毛膜促性腺激素(β-HCG)单克隆抗体。方法 以SnCl2为还原剂,采用直接标记法进行标记。结果 整个标记过程约需1.5h,抗体标记率为88.1%-97.5%,胶体含量为0.96%-1.27%,游离锝含量为1.10%-9.92%,且抗体的生物活性未受到影响。结论 该法简单、快速,有望用于妊娠滋养细胞疾病的放射免疫显像。
Abstract:
Objective To label the anti-β-human chorionic gonadotropin monoclonal antibody with β. Methods It was direct labeled using SnCl2 as a reduction agent. Results The labeling procedure was finished within 1.5 hours. The labeling efficiency of the labeled anti-β-human chofionic gonadotropin monoclonal antibody was 88.1%-97.5%, 99mTc colloidal was 0.96%-1.27%, 99mTcO4- was 1.1%-9.92%. And it retained its specific biological activation. Conclusion This direct method is simple, rapid and stable, and it hope to be used as radioimmunodiagnosis for gestational trophoblastic turnout.

参考文献/References:

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3 Oliva JP, Pimentel G, Borron M, et al. Pilot study with the monoclonal antibody IOR-C5 as a potential agent of radioimmunoscintigraphy in colorectal cancer. Rev Esp Med Nucl, 2001, 20(4):282-288.
4 Alauddin MM, Khawli LA, Epstein AL. An improved method of direct labeling of monoclonal antibodies with 99mTc. Int J Radiat Appl Instrum B, 1992, 19(4):445-454.
5 Rhodes BA, Zamora PO, Newell KD, et al. Direct 99mTc labeling of murine monoclonal antibody fragments. J Nucl Med, 1986, 27(5):685-693.
6 张金赫,徐海峰,邵秋菊,等.99Tcm直接法标记血管抑素.第四军医大学学报,2003,24(23):2189-2192.
7 Griffiths GL, Goldenberg DM, Jones AL, et al. Preparation of a pure 99mTc-F(ab’)2 radioimmunoconjugate by direct labeling methods.Nucl Med Biol, 1994, 21(4):649-655.

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备注/Memo

备注/Memo:
收稿日期:2006-07-16。
基金项目:陕西省科学技术研究发展计划基金资助项目(2003k10-G47)
通讯作者:安瑞芳(E-mail:anruifang@163.com)
更新日期/Last Update: 1900-01-01