[1]蔡常辉,梁锦胜.乙型肝炎e抗原、前S1抗原、前S2抗原与乙型肝炎病毒DNA关系探讨[J].国际放射医学核医学杂志,2006,30(4):232-234.
　CAI Chang-hui,LIANG Jin-sheng.Correlation between the e-antigen, Pre-S1 antigen, Pre-S2 antigen and DNA of hepatitis B virus[J].International Journal of Radiation Medicine and Nuclear Medicine,2006,30(4):232-234.
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《国际放射医学核医学杂志》[ISSN:1673-4114/CN:12-1381/R]

卷:
30
期数:
2006年第4期
页码:
232-234
栏目:
临床核医学
出版日期:
1900-01-01

文章信息/Info

Title:
Correlation between the e-antigen, Pre-S1 antigen, Pre-S2 antigen and DNA of hepatitis B virus
作者:
蔡常辉;  梁锦胜
528403 广东中山, 广东省中山市人民医院检验中心
Author(s):
CAI Chang-hui;  LIANG Jin-sheng
The laboratory Center of the People’s. Hospital of Zhongshan City, Zhongshan Guangdong 528403, China
关键词:
乙型肝炎病毒DNA; 乙型肝炎e抗原; 前S1抗原; 前S2抗原
Keywords:
Hepatitis B virus DNA; HBeAg; Pre-S1 antigen; Pre-S2 antigen
分类号:
R512.6+2
摘要:
目的 探讨乙型肝炎e抗原(HBeAg)、前S1抗原(Pre-S1)、前S2抗原(Pre-S2)与其病毒DNA(HBVDNA)的关系。方法 收集268例乙型肝炎患者血标本,以荧光定量PCR法检测HBVDNA,时间分辨荧光免疫分析法检测HBeAg,酶联免疫吸附法检测Pre-S1、Pre-S2。结果 HBVDNA阳性组HBeAg、Pre-S1、Pre-S2阳性率分别为48.2%、76.4%、100%,HBVDNA阴性组分别为1.6%、36.3%、32.3%,两组间每指标阳性率比较,差异均有显著性(均P<0.01)。结论 HBeAg、Pre-S1、Pre-S2与HBVDNA关系密切,以HBVDNA阳性为参照标准,反映病毒复制的敏感性Pre-S2最高,其次为Pre-S1,HBeAg较低;Pre-S1、Pre-S2可作为HBVDNA的补充指标。
Abstract:
Objective To study the relationship between the hepatitis B e-antigen (HBeAg), Pre-S1 antigen (Pre-S1), Pre-S2 antigen (Pre-S2) and DNA of hepatitis B virus (HBV). Methods The blood samples of 268 cases of viral B hepatitis were collected. The HBV DNA of all samples were tested by fluorescent-quantitating PCR method, and HBeAg were assayed by time-resolved fluoroim-munoassay method, and their Pre-S1 and Pre-S2 were assayed by enzyme linked immunosorbentassay method. Results The positive rates of HBeAg, Pre-S1 and Pre-S2 in HBV DNA positive group were 48.2%, 76.4% and 100% respectively, and 1.6%, 36.3% and 32.3% respectively in HBV DNA negative group. There was significantly difference between the HBeAg, Pre-S1 and Pre-S2 positive rates of the two groups (Chi-square test, P<0.01). Conclusions There was positive relationship between the HBeAg, Pre-S1, Pre-S2 and DNA which all were indicators of HBV reproduction. Comparing to HBV DNA, Pre-S2 was the most, Pre-S1 the second, and HBeAg the third sensitive indicator for evaluating HBV reproduction. Pre-S1 and Pre-S2 could be used as the supplementary indicator for the reproduction of HBV.

参考文献/References:

1 闵福援,孙桂珍,王健,等.前S1蛋白在乙型肝炎诊断及判断预后中的作用.中华检验医学杂志,2004,27(4):224-226.
2 姚集鲁,杨绍基,高志良.传染病学临床专论.第1版,广东:广东高等教育出版社,2000.1-8.
3 关秀茹,张萱,韩丽,等.乙型肝炎病毒前S1抗原在乙型肝炎病情诊断及预后判断中的应用.哈尔滨医科大学学报,2003,37(1):80-81.
4 周岳进,肖扬,王开鉴.微流芯片检测HBV前C区nt1896/BCP区nt1762基因突变的临床意义.实用肝脏病杂志,2005,8(5):276-277.

备注/Memo

备注/Memo:
收稿日期:2006-02-18。
通讯作者:蔡常辉,E-mail:caichanghui@163.com
更新日期/Last Update: 1900-01-01